酶蛋白

Methods:The whole mature protein coding sequence of three truncated dystrophin gene was amplified by RT-PCR method applied to human muscle cDNA. The fragment was inserted into prokaryotic expression vector pET28a plasmid.

以人肌肉组织cDNA为模板，采用RT-PCR扩增三个截短的肌营养不良蛋白cDNA，分别克隆入原核表达载体pET28a中，经限制性内切酶双酶切及DNA序列分析鉴定目的基因。

Laboratory diagnostic markers of acute pancreatitis in children include amylase and Isozyme,lipase,c-reactive protein,urinary trypsinogen2,trypsinogen activation peptide,procalcitonin and so on.

小儿急性胰腺炎的实验室诊断标志物包括淀粉酶及同工酶、脂肪酶、C反应蛋白、尿胰蛋白酶原2、胰蛋白酶原激活肽、降钙素原等。

Objective To explore the clinical value of the titer of serum HP antibody in patients with gastric diseases.Methods Serous HP antibody was detected with ELISA and HP was cultured,tested with urase,and stained with Gram method on smears which was observed microscopically and compared with proteus protein-enveloped test.Results ELISA showed high sensitivity and specificity.Conclusion Serous ELISA detection of HP antibody can be used widely in clinical diagnosis and epidemical screening without endoscopy and biopsy.

目的 探索胃部疾病患者血清中的幽门螺旋菌HP抗体滴度的临床价值方法应用酶联免疫吸附试验ELISA法检测HP抗体与HP培养，尿素酶试验和涂片革兰氏染色作镜下形态检查相比较，并同时与变形杆菌蛋白包被测定法作比较结果显示该法具有敏感特异性强的特点结论该法无需常规内镜检查，活检查HP而单纯用血清学检查，可广泛应用于临床诊断和流行病学人群普查

Further analysis indicated that only in the first 12 hours after the hormonal stimulation, the addition of vanadate to the cells blocked the cellular differentiation process.

蛋白酪氨酸磷酸酯酶HA2（PTPase，HA2），一个PTPase 1B的同源酶，在3T3-L1前脂肪细胞诱导分化成脂肪细胞的调控过程中起着重要作用。

It was found the levels of both large and small subunits were low at the albescent stage and became normal after leaves recovered to green, which corresponded with the enzyme activity change.

发现安吉白茶在返白与复绿过程中叶片可溶性蛋白的主要变化是RuBP羧化酶大、小亚基含量上的差异，这种差异与RuBP羧化酶活性的变化是相一致的。

The variation of large and small subunits of RuBPcase from temperature-sensitive mutant of Anjibaicha during its stage albescent process was investigated as well as the changes of the enzyme activities.

利用SDS-PAGE电泳分析了返白和复绿过程中各阶段叶片可溶性蛋白组分，尤其是RuBP羧化酶大、小亚基的变化，并利用同位素法测定了此过程中RuBP羧化酶活性的变化。

It participates to make up antioxidase and many other selenium proteins that exert the effects in eliminating free radical and antioxidation in human body.

硒是人体必需微量元素，它参与抗氧化酶谷胱甘肽过氧化物酶和多种硒蛋白的组成，在体内发挥消除自由基、抗氧化物等作用。

Methods The level of serum and urinary tissue-type plasminogen activator and plasminogen activator inhibitor-1 (PAT-1) were measured in 38 patients with chronic glomerulonephritis, 28 patients with nephropathy syndrome, 36 patients with chronic renal failure and 20 healthy subjects by means of enzyme-linked immunosorbent assary. The relationship among urinary t-PA, PAI-l and serum t-PA, PAT-1, urinary protein, serum creatinine were also observed. Results Levels of serum t-PA, PAI-1 in chronic kidney diseases increased remarkably compared with control group.

选择38例慢性肾小球肾炎，28例肾病综合征，36例非透析治疗的慢性肾功能不全和20例正常对照作为研究对象，应用ELISA法检测血清和尿液中组织型纤溶酶原激活剂和纤溶酶原激活物抑制剂－1(PAI-1)的浓度，同时分析尿中t-PA和PAI-1的水平与血t-PA、PAI-1、血肌酐和24h尿蛋白总量之间相关性。

After cultivating in liquid medium for 5 days, an extra cellularβ-mannanase from Athelia rolfsii strain CBS191.62 was purified 15.1-fold toelectrophoretic homogeneity by ammonium sulfate precipitation, DEAESepharose Fast Flow chromatography, Hydroxylapatite chromatography andrefrigeration crystal.

产β-甘露聚糖酶的Athelia rolfsii菌株CBS 191.62，发酵培养5d，发酵液离心去菌体，上清经硫酸铵沉淀，琼脂糖凝胶层析、羟基磷灰石层析和冷冻干燥结晶等步骤，β-甘露聚糖酶的比活提高了15.1倍，获得凝胶电泳均一的蛋白样品。

The paper proposed a possible binding mode of MS-275, a benzamide histone deacetylase inhibitor, to HDAC by intensive docking study.

通过计算机模拟的对接过程研究，发现了MS-275--一种苯甲酰胺类的组蛋白去乙酰酶抑制剂与酶的可能的全新结合方式。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。