酶蛋白

To study the expression of hTERT, p53 and their relations in gastric cancer and precancerous lesions.

目的探讨端粒酶逆转录酶基因、p53蛋白在胃癌及癌前病变中的表达及相关性。

Methods HBV DNA was extracted from the serum of patient with hepatitis B. The X gene was amplified by PCR using the primers with EcoRI and HindⅢ digestion sites, and then cloned into pronucleus expression vector pMAL-C2X, which was detected by EcoRI and HindⅢ digestion and sequence.

从乙肝病人血清中提取HBV DNA；以带有EcoRI和HindⅢ酶切位点的引物，用PCR方法扩增X基因；而后定向克隆到原核表达载体pMAL-C2X上，经酶切鉴定和序列分析；以IPTG诱导X融合蛋白的表达。

The result showed that Ref1 was a psychrophile, and the best growing conditions were pH 6, 20℃ and yeast extract as nitrogen source while the best xylanase producing conditions were pH 3－7, 15℃ and yeast extract as nitrogen source.

结果表明：该菌为低温型菌株，其最佳生长条件为pH6、20℃和酵母膏作为氮源；最佳产酶条件为pH3－7、15℃及以酵母膏氮源；条件优化后产酶可达118.7U/mL，可溶蛋白含量可达到60？

The results of gold immunolocalization showed the Rubisco distributed in the pyrenoid (90-95%) and starch sheath region (4-8%), only few Rubisco located in the stroma of chloroplasts.

莱茵藻和条浒苔Rubisco活化酶分子定位结果也表明Rubisco活化酶主要分布于蛋白核表面和淀粉鞘及淀粉粒区中。

In the feedback regulation system of choles-terol metabolism, SCAP together with membrane-bound transcription factors - sterol regulatory element-binding proteins control transcription of the genes encoding enzymes of choles-terol and fatty acid biosynthesis.

在胆固醇代谢的反馈调节系统中，SCAP与膜结合转录因子胆固醇调节元件结合蛋白(sterol regulatory element binding proteins；SREBPs)等调节因子，共同控制一系列酶编码基因的转录过程，包括胆固醇和脂肪酸生物合成过程中所需的酶。

YIQIHUOXUEFANG can relieve the myelin sheathe swollen of the nerves and the disease of the sciatics, and promote local vessel density around the myelin sheathConclusionsYIQIHUOXUEFANG can regulate blood glucose effectively, improve the mico-circulation around the sciatics of the diabetes rats, restrain the metabolism of the multi-alcohol, increase the expressions of the NGFand MBP, i.e.

益气活血方能有效地调节血糖，改善DM大鼠坐骨神经周围微循环，抑制多元醇代谢，有醛糖还原酶抑制剂样作用，升高Na~+-K~+-ATP酶活性，改善和提高神经传导功能，促进坐骨神经神经生长因子和碱性髓鞘蛋白的表达，可明显缓解DM大鼠周围神经的早期病变，有一定的神经修复作用，综合疗效优于弥可保。

Then the recombinant plasmid transformed E.coli.JM109. One positive clone was obtained from the libraries with casein flat and ELISA sieve method.

用酪蛋白平板法和酶联免疫吸附的活性筛选方法从基因文库中筛选到一株产海洋低温碱性酶的阳性克隆（命名为pHH1）。

To sodium nitroprusside as nitric oxide donor, was studied for different concentrations of NO Toyonoka strawberry fruit post-harvest ethylene production, respiration rate, vitamin C, soluble protein, water loss and relative conductance decay rate, as well as superoxide dismutase, catalase and peroxidase activity.

摘 要：以硝普钠为一氧化氮供体，研究了不同浓度NO对丰香草莓采后果实乙烯产生、呼吸强度、维生素C、可溶性蛋白、失水腐烂和相对电导率，以及超氧物歧化酶、过氧化氢酶和过氧化物酶活性的影响。

METHODS: Fresh bovine tendo calcaneus collagen was dispersed in 0.5 mol/L acetic acid, co-precipitated with chitosan and lyophilized. Dry membranes were cross-linked in 0.05% glutaraldehyde for 24 hours. In vitro its degrading rate was measured by use of collagenase degrading test. The chitosan-collagen membrane was implanted to subcutaneous dorsal sites of SD rats. After implantation, histocompatibility, vascularity and degradation were observed in vivo.

以酸溶法制备胶原溶液，添加一定比例的脱乙酰化几丁质，冻干成膜，0 。0 5 %戊二醛溶液浸泡交联，行体外酶降解实验，检测其耐受酶降解能力；将制备的几丁质-胶原蛋白膜包埋于 36只 SD大鼠皮下，术后 3、5天，1、2、3、4、6、8和 12周取材，检测其组织相容性、血管化能力及材料在体内降解情况。

They also had a more adverse atherothrombotic profile, with higher tissue-type plasminogen activator and plasminogen activator inhibitor-1 (PAI-1) antigen.

他们也具有更不利的动脉粥样化血栓形成的曲线：更高的组织纤溶酶原激活物和纤溶酶原激活物蛋白抑制剂1 (PAI-1)抗体。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。