酶蛋白

Meanwhile, Tibetan sheep (breeded in Sanjiaocheng of Qinghai, Huangcheng and Gannan of Gansu ), Mincounty Blank Fur sheep and Texel and Poll Dorset were controlled. The results indicated that polymorphism was tested at the loci of Hb, Tf and Es, that erythrocyte protein 1 and 2 were monomorphism. There were a couple of allele and three genotypes at the loci of Hb and Es. There were 10 genotypes at the locus of Tf, which was determined by 5 alleles.

结果表明，青海细毛羊和甘肃高山细毛羊的血红蛋白、转铁蛋白和酯酶基因座上存在丰富的多态性，其中Hb和Es位点受一对等位基因控制，共构成3种基因型；Tf位点受5个等位基因控制，共构成10种基因型；而红细胞蛋白质-1（EP-1）和红细胞蛋白质-2（EP-2）基因座均呈显单态；青海细毛羊的AMY基因座具有AMY1、AMY2、AMY3三种同工酶，其中AMY2呈多态，有AMY2A和AMY2O两种表现型，而甘肃高山细毛羊的AMY呈显单态。

It shows us that the sufu can also treated as functional food. The biochemical process keeps unknown during sufu making. My study focuses on preparation, purification and hydrolysis properties of protease of Mucor piriformis Fischer which is a common used strain for process sufu. The main results are followed.

因此本论文选用用于腐乳生产的菌种梨形毛霉作为蛋白酶的生产菌种，从蛋白酶酶制剂的制备方法、性质的测定以及蛋白酶的分离纯化到该酶对大豆分离蛋白的降解作用逐一进行了研究，获得了以下主要结果。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

Objective To clone human ornithine decarboxylase antizyme mutation gene and express its recombinant protein.

目的 构建人鸟氨酸脱羧酶抗酶突变基因，重组至原核表达载体中并表达出重组蛋白。

The specific activity of pectate lyase was 15 U/mg crude protein.

细胞经超声破碎后，测得果胶酶的比酶活为15U/mg粗蛋白。

Three proteins in the tumor cells transport enzymes needed to perforate this barrier, and another protein puts these enzymes in the right place.

在肿瘤细胞转运酶中有三种蛋白质是刺穿这种屏障所必需的，另一种蛋白能把这些酶运送到合适的位置。

Results hPDLP wasisolated from human periodontium and most of the cells retained their fibroblastic spindle shape. hPDLP can be in-duced into osteoblast-like cells and adipocyte-like cells, and calcium deposition and lipid droplets were detected perspectively.

结果 第1代hPDLP的CD146和STRO-1阳性率分别是27.20%±3.98%和4.23%±4.08%；经矿化诱导可以形成矿化结节，有钙盐沉积；经成脂诱导可见特异性脂滴形成，特异性转录因子过氧化物酶体激活物增生受体2（PPARγ2）和脂蛋白脂酶表达上调。

So, is the cAMP protein kinase and phosphorylase a key enzyme system .

所以蛋白激酶是cAMP与磷酸化酶系统中一个关键的酶。

The seeds are stratified under lab condition. The average of high temperature is 19.36℃(2～4 months), the embryo completes the differentiation of the seed leaf, the radicle and plumule in this period.

在层积过程中，定期测定了种子的呼吸速率，可溶性蛋白、还原糖、粗脂肪和有机酸的含量，同时还测定了过氧化物酶和过氧化氢酶的活性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。