酶蛋白

The complex of flavourzyme and neutral protease was used to hydrolyze camellia pollen to obtain bioactive peptides.

茶花花粉蛋白经过风味酶和中性蛋白酶组成的复合酶水解得到活性肽。

The complex of flavourzyme and neutral protease was used to hydrolyze protein from camellia pollen to obtain bioactive peptides.

茶花花粉蛋白经过风味酶和中性蛋白酶组成的复合酶水解得到活性肽，并进一步研究活性肽的抗氧化特性。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

A double-antibody sandwich ELISAemploying monoclonal antibody 5G5 was developed for detection of P27 antigen of avian leucosis virus.The limit of viral antigens detected was 5?

利用原核表达的禽白血病病毒P27蛋白作为抗原制备的单抗作为包被抗体，以制的酶标兔抗P27作为酶标抗体，在国内首次建立了检测ALV抗原的双抗体夹心ELISA方法。

The robustness of this model is discussed by using the S-system model. And the influences of cell growth rate on the biosynthesis of tryptophan, stability and dynamic behavior of the trp operon are investigated. A steady-state optimization model is established. It maximizes the accumulative term accounting for both consumption and secretion. The nonlinear optimization problem is approximately transformed to a linear optimization problem.

本文对表达阻遏蛋白的基因和表达合成色氨酸关键酶的基因之间相互作用进行了研究，通过模型考察了比生长速率对色氨酸生物合成和系统稳定性的影响，对色氨酸合成过程所涉及到的mRNA、酶等的动态行为进行了理论预测和分析，通过S-系统方程将非线性系统转化为线性系统，研究了系统的鲁棒性，并对色氨酸生产进行了优化。

Objective To determine the possible role of hepatic lipase and lipoprotein lipase in the pathogenesis of fatty liver.

目的 探讨肝脂酶和脂蛋白脂肪酶在脂肪肝发病中的作用。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

A substrate concentration of 0.277 kg/L, enzyme/ substrate level of 1408 U/g, temperature of 39.2 ℃, and a hydrolysis time of 4.86 hour were found to be the optimum conditions for high antibacterial activity. Under this condition, the bacteria Escherichia coli, Micrococcus luteus and Bacillus subtilis in the hydrolysates had the inhibitory rate of 71.40%, 56.88%, and 64.54%, respectively.

经Design Expert 7.1.2软件优化，得出胃蛋白酶酶解鲶鱼骨蛋白的最优条件为：底物浓度0.277kg/L，加酶量1408 U/g，水解温度39.2℃，水解时间4.86 h，在此条件下获得的水解物，对大肠杆菌、藤黄微球菌及枯草芽孢杆菌的抑菌率分别为71.40%、56.88%和64.54%。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。