酶蛋白

The protein and phospholipid constituent in membrane of neurocyte in brain of chicken with encephalomalacia was separated and studied with the method of SDS-PAGE electrophoresis and the method of high performance-thin layer chromatography respectively The fluidity of neurocyte membrane of chicken with encephalomalacia was determined for the first time The content of Ca and trace elements were determined in chicken encephalomalacia systematically and the status of Fe ion in cerebellum was observed. The activity of enzymes that were relative to metabolism of energy and neuro-medium in brain were checked systematically with the method of enzyme-histochemistry. Pathological examination was carried out by microscopic and ultramicroscopic observation. The apoptosis of neurocyte in brain was checked.

在进行临床观察、定期剖杀并检测体内V〓含量、体重和脑重的基础上，重点研究了与神经损伤有关的以下几个方面的内容：系统地检测了体内自由基代谢和抗氧化系统功能的变化；首次采用SDS-PAGE电泳法分离并检测了雏鸡脑软化症神经细胞膜蛋白组分，并以高效薄层层析法检测神经细胞膜磷脂组分；测定了脑软化症雏鸡神经细胞膜的膜流动性；首次系统检测了脑软化症雏鸡体内钙及微量元素含量，并进行了小脑组织总铁离子状态观察；采用酶组织化学方法系统地观察了脑组织中与脑能量代谢和神经递质有关的酶的活性；对相关组织进行了病理组织学和超微结构观察；并对脑组织中神经细胞的凋亡进行了检测。

Activity of Diamine oxidase: DOA is the highly active endoenzyme in the microvilli of small intestine of human and other mammal, which activity has crucial correlation with nucleic acids and protein synthesize within mucous cells and also reflects the completion or trauma level of small intestinal mucosa.

二胺氧化酶活性：DAO是人类和哺乳动物小肠粘膜上层绒毛中具有高度活性的细胞内酶，其活性与粘膜细胞的核酸和蛋白合成密切相关，可反映小肠粘膜的完整性和损伤程度。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

Then soybean milk was hydrolyzed by Enzyme B at the optimum enzymolysis conditions.

本实验研究了在不同条件下用三种酶酶解豆浆中蛋白的程度。

Results There were significant differences between MDS and CAA in Hb, red cell distribution width-coefficient variation, immature reticulocyte fraction, BPC, the ratio of G1 (the sum percentage of myeloblast and premyelocyte) to G2 (the sum percentage of neutrophilic myelocyte and metamy-elocyte), the ratio of E1 (the sum percentage of proerythroblast and early erythroblast) to E2 (the sum percentage of intermediate erythroblast and late erythroblast), megakaryocyte count,erythroblast PAS, neutrophil alkaline phosphatase, and serous levels of indirect bilirubin,lactose dehydrogenase, folic acid, VitBl2 and ferritin.

结果 MDS患者血红蛋白，红细胞体积分布宽度。变异系数、未成熟网织细胞比率、血小板计数、骨髓原始细胞及早幼粒细胞之和与中性中幼粒细胞及中性晚幼粒细胞之和的比值、原始红细胞及早幼红细胞之和与中幼红细胞及晚幼红细胞之和的比值、巨核细胞计数、有核红细胞糖原染色阳性率和阳性指数、中性粒细胞碱性磷酸酶染色阳性率和阳性指数、血清间接胆红素、乳酸脱氢酶、尿酸、叶酸、维生素B12(VitB12)、铁蛋白水平等常规实验室指标与CAA患者比较差异有统计学意义。

We obtained a 1.5Kb DNA fragment of the succinate dehydrogenase flavoprotein subunit by PCR amplification and carried out the homolog analysis with those of other organisms.The result indicated a high homologh in this fragment among the five organisms.

通过PCR方法得到了琥珀酸脱氢酶黄素蛋白基因的一个1.5KB大小的DNA片段，并与其它生物的同种酶的基因序列进行了同源性分析，发现在所比较的五种不同生物之间这个区段具有很高的同源性。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

Ribose/galactose/methyl galactoside importing ATP-binding protein 1 was participated in photosynthates transportation from leaf to grain in early and mid-stages.

分析结果表明，核糖／半乳糖／甲基半乳糖苷运输ATP结合蛋白l在灌浆前中期参与物质向籽粒的运输；生长素响应蛋内IAA27通过调节ATPase活性影响叶片物质运输；N-乙酰谷氨酸半醛脱氢酶在灌浆末期参与叶片的多胺代谢，延缓叶片衰老；谷胱甘肽S-转移酶和Cu/Zn-超氧化物歧化酶在籽粒灌浆后期的植物解毒和防御活性氧伤害中起着重要作用。

Objective to research the changes in expression of heme oxygenase-1 mRNA and protein during atherosclerosis development.

目的 研究在动脉粥样硬化发病过程中内源性一氧化碳的合成酶血红素氧化酶-1mRNA和蛋白表达的变化规律。

Galactosyltransferase 1 is the first cloned glycosyltransferase. It had been regarded as a house-keeping gene for a long time. In our previous investigation, it was found that GaT I was an intraction protein of SGT, and EIAF, one of the Ets family members could regulat its expression.

1，4半乳糖基转移酶是一个最早克隆的糖基转移酶，长期以来被认为是一个管家基因，本实验室以前的工作表明GalT Ⅰ是SGT的一个相互作用蛋白，Ets转录因子家族的成员EIAF可调控其基因的转录。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。