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The expressions of HO-1 and HO-2 mRNA were detected using reverse transcription polymerase chain and the relative amount of CO released into the media was quantitated as carboxyhemoglobin by enzyme linked immunosorbent assay.

通过逆转录聚合酶链式反应和免疫细胞化学法检测HO-1、HO-2 mRNA和蛋白的表达,酶联免疫法检测培养上清液中碳氧血红蛋白含量,四唑氮比色法检测细胞增殖。

This key reaction is catalyzed by Ribulose 1,5-bisphosphate carboxylase/oxygenase, which is the most abundant protein on earth.

这步关键反应被1,5-二磷酸核酮糖羧化/加氧酶( Ribulose 1,5-bisphosphate carboxylase/oxygenase ,Rubisco)所催化,该酶是世界上最丰富的蛋白。

Ribusco-1,5-bisphosphate carboxylase/oxygenase located in the chloroplast is the most abundant protein in the leaves of light-grown plants.

1,5-二磷酸核酮糖羧化酶/加氧酶位于植物叶绿体中,是绿色植物中含量最丰富的蛋白,它催化CO2固定和光呼吸作用的第一步。

The method controls the catalytic speed of the microencapsulation glucose oxidase through adjusting the molecular weight and the degree of deacetylation of microcapsule exine chitose, thereby the method is beneficial for oxidation-linking of gluten protein and for increasing the quality of the flour and flour products, simultaneously, the glucose oxidase after microencapsulation avoids the contact between enzyme and the flour and improves storage and the stability, and the microencapsulation glucose oxidase is excellent flour modifying agent and accords with practical production and application.

该方法通过调节微胶囊外壁壳聚糖的分子量和脱乙酰度,控制微胶囊内葡萄糖的催化速度,从而有利于面筋蛋白的氧化交联,提高面粉及其制品的品质;同时葡萄糖氧化酶经微胶囊化以后避免了酶与面粉的接触改善了贮藏和稳定性,是优良的面粉改良剂,符合实际生产应用。

Using ultrasonic to measure the thickness of the cholecyst cliff in these patients. At the same time, we measure their breadth of the portal vein and the level of the ascites, and examine the level of ALB、TBil、AST、ALT in blood serum.

采用超声观察178例肝硬化患者的胆囊壁厚度和腹水检查、门静脉宽度、血清白蛋白水平、总胆红素水平以及门冬氨酸氨基转移酶、丙氨酸氨基转移酶水平。

The results demonstrated that the measured molecular weight of phycocyanin was 37.715Kd, and consisted of two peptides which were similar with their molecular weight and amino acid sequences.4 The comformation of phycocyaninPhycocyanin high structure was investigated, as the result of circular dichroism spectrum and Fourier transformation infared spectrum shown, the secondary structure of NSPC was mainlyα-helix structure and the content ofβ-shee or coil was very small.

根据氨基酸自动分析仪测定的氨基酸组成和精确分子质量计算出NSPC分子总共有331个氨基酸残基,分别是由40个Asp、22个Thr、18个Ser、22个Glu、1个Gly、31个Ala、27个Cys、17个Val、13个Ile、22个Leu、5个Tyr、7个Phe、7个Lys、24个His、51个Arg、14个Pro、1个Met和9个Trp组成。胰蛋白水解酶分别酶解NSPC-1和NSPC-2后,分别采用MALDI-TOF-MS分析,其肽指纹谱图显示胰蛋白酶可将NSPC-1和NSPC-2水解成大小不等的多条肽段,质量数在800以上的NSPC-2有11段,即水解的肽质量分别为899.54、1454.77、1544.9、1683.87、1803.93、1811.91、1987.92、2003.90、2536.26、2915.42和3492.72。

MATERIALS AND METHODS: Formaldehyde, an oxidative deamination product of methylamine, was analyzed by HPLC.

材料与方法:利用弱阴离子交换柱层析分离兔血浆蛋白,测定各收集组分的SSAO酶活性;以甲胺为底物测定SSAO酶动力学参数。

In this project, in order to develop a designable and controllable mixed microbial culture with intelligent signals, we chose the alkalophilic mixed culture as model system for production of multienzymes consisting of alkaline cellulase and alkaline amylase, and the basic characteristics of the mixed culture was examined.

本项目选择了能够生产碱性淀粉酶和碱性纤维素酶的嗜碱微生物复合体系来生产复合酶,重点研究嗜碱菌复合培养过程的特点及基于荧光蛋白基因快速定量解析复合培养体系的组成菌种及其相互作用的方法论基础。

Enzymatic activity assay of oxalate oxidase in transgenic tobacco plants indicated that the germ in gene from monocotyledon, could be expressed in dicotyledon tobacco plants that resu;ted in the formation of homopentameric protein with enzymatic activity correctly.

酶活性检测表明,源于单子叶植物小麦的germin基因能够在双子叶植物烟草中表达并正常装配成具有酶活性的同源五聚体蛋白。

The enzymes enzyme screening and solution egg albumin of the appropriate conditions

还有这句:并筛选出该酶酶解鸡蛋清蛋白的适宜条件不要google自动生成的哈。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。