酶蛋白

Some biochemical properties of cathepsin B, D, E, Hand L, calpain I and calpain II, dipeptidyl peptidase I, II, III and IV, and alanyl-, arginyl-, leucyl, and pyroglutamyl aminopep-tidases were introduced and their roles in meat texture were discussed.

分述了组织蛋白酶B， D， E， H 和L、肌钙蛋白I、II、二肽基肽酶 I， II， III ，IV、丙氨酰、精氨酰、亮氨酰及焦谷氨酰氨基肽酶以及它们在肉的质构中的作用。

Methods: MTT and enzyme dynamics methods were used to observe the effects of CAP on cell proliferation and alkaline phosphatase activity of HDFC.

分别采用MTT比色法和酶动力学方法观察牛牙骨质附着蛋白对体外培养人牙囊细胞增殖及其碱性磷酸酶活性影响。

The Effects of ED -71 on proliferation and differentiation were detected by MTT,.cell cycle, alkaline phosphatase activity and level of osteocalcin.

采用细胞形态学，碱性磷酸酶染色，培养细胞匀浆上清测定单位蛋白下的碱性磷酸酶活性，培养基中骨钙素含量分析及原位杂交方法检测ED-71对OS-732人成骨样细胞分化的影响。

Results Agarose gel electrophoresis proved that the length of PCR product was about 7 000 bp. SDS-PAGE showed that the target protein was highly expressed. Compared with those of control, the anthranilate synthase and tryptophan synthase activities of expressed product increased by 2.7 and 3.2 folds respectively.

结果 凝胶电泳可见代PCR扩增产物大小约为7000bp，SDS-PAGE鉴定目的蛋白分别得到了表达，邻氨基苯甲酸合成酶和色氨酸合成酶的活性分别比对照提高了2.7和3.2倍。

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

It was also demonstrated the inhibitors could inhibit the activities of enzymes to a great degree, the bacitracin and casein showed the prominent effect.

同时也证明了加入酶抑制剂能够对rHV2提供很大程度的保护，其中杆菌肽和酪蛋白的酶抑制作用比较突出。

Methods: According to their urinary albumin excretion rate, Diabetes mellitus patients with micrangium disease were further divided into three subgroups: normo-albuminuria、micro-albuminuria and clinical proteinuria; The expression of platelet membrane α-glucoprotein (CD62p) and cytolysosome protein (CD63)、platelet aggregation function and mean platelet volume was determined with flow cytometer、blood aggregation analyzer、automated blood cell counter (MEK-6318K) respectively, and compared with 30 normal controls.

对糖尿病微血管病变患者按尿清蛋白排泄率分为正常清蛋白尿组、微量清蛋白尿组、临床清蛋白尿组三组，分别用流式细胞术、血液聚集仪、全自动血细胞分析仪测定血小板内α-颗粒膜蛋白(CD62p)、溶酶体蛋白(CD63)的表达及血小板聚集功能和平均体积，并与30例正常对照组比较。

Methods Lewis rats were immunized with the protein extracted from electric organ of Narcine timilei, the clinical manifestations were observed every day, and repetitive nerve stimulation,single fiber electromyography and the levels of acetylcholine receptor antibody were detected on the 49th days post immunization. The content of the protein extracted from electric organ of Narcine timile was 35.4 mg/mL, and the protein could respond with AChRAb from the serum of seropositive myasthenia gravis patient.

参照徐氏法从丁氏双鳍电鳐的电器官提取AChR蛋白，并采用Folin-酚试剂法及酶联免疫吸附法检测提取蛋白的含量及活性；以提取的蛋白主动免疫Lewis大鼠，每天观察其临床表现，并于免疫后7周进行低频重复电刺激、单纤维肌电图及血清中AChRAb水平的检测。

Methods Lewis rats were immunized with the protein extracted from electric organ of Narcine timilei, the clinical manifestations were observed every day, and repetitive nerve stimulation, ingle fiber electromyography and the levels of acetylcholine receptor antibody were detected on the 49th days post immunization. The content of the protein extracted from electric organ of Narcine timile was 35.4 mg/mL, and the protein could respond with AChRAb from the serum of seropositive myasthenia gravis patient.

参照徐氏法从丁氏双鳍电鳐的电器官提取AChR蛋白，并采用Folin-酚试剂法及酶联免疫吸附法检测提取蛋白的含量及活性；以提取的蛋白主动免疫Lewis大鼠，每天观察其临床表现，并于免疫后7周进行低频重复电刺激、单纤维肌电图及血清中AChRAb水平的检测。

As estimated by SOS-PAGE the molecular weight of PG was 31500, lower than that of PG in other fruits. The most pH stability was at a range of pH 4.5-8.0. The most optimum ion strength was at 0.O4mol/L-0.O6mol/L. The U.V. spectrum of PG was about 267 nm.

经SDS-PAGE测得枣PG蛋白的分子量是31500，比其它果实中的PG蛋白的分子量小；PG蛋白的pH稳定范围是4.5-8.0；最适离子强度范围是0.04 mol/L-0.06mol/L；PG酶的最大紫外吸收光谱是267nm。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。