酶蛋白

The following data were obtained and compared between resistance and responder: white blood cell,neutrophil,hemoglobulin,C-reactive protein,erythrocyte sedimentation rate,albumin,and lactate dehydrogenase.Male patients with decreased albumin may be related with resistance,but they could not be good predictors of resistance.

川崎病患儿发生IVIG耐药的可能危险因素有外周血白细胞、中性粒细胞比例、血红蛋白、C反应蛋白、血沉、血浆白蛋白、乳酸脱氢酶等，分析结果提示血浆白蛋白降低及男性可能与IVIG不敏感川崎病有关，但仅此两项指标尚不能预测IVIG不敏感川崎病的发生。

The Pinenut slurry was hydrol yzed with Neutrase(AS1398)under the selected conditions(50℃,pH8 or 9,1h)before being homogenized.The desired DH could be achieved by this procedure without sp ecific enzyme inactivation process.

实验采用AS1398中性蛋白酶水解松子浆料，水解进程曲线表明，当加酶量为 0.5%～1.5%，水解温度50℃、pH8或9时，水解1h后酶基本完全失活，可实现在无专门灭酶操作情况下控制蛋白水解度。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

METHED We selected 40 patients underwent coronary angiography. Blood samples for measurement of CRP, Cardiac Troponin I, Creatine Kinase, MB isoenzyme of CK and lipoidemia were taken in the morning just before angiography in all patients. Each subject was sought details of sex, age. Body Mass Index, history of smoking, hypertension, diabetes mellitus.

方法选取住院并接受冠状动脉造影(coronary angiography， CAG)的患者40名进入本研究，均于CAG前采集空腹血，检验C反应蛋白、肌钙蛋白Ⅰ(Troponin Ⅰ，TnⅠ)、甘油三酯、总胆固醇、高密度脂蛋白胆固醇、肌酸激酶、肌酸激酶同工酶MB，并收集所有患者年龄、年龄、高血压史、糖尿病史、吸烟史、体重指数的资料。

Then pMD-P80 is digested by the enzymes Xho I and Apa I, separated and linked to the linear plasmid pEGPF-C1 after digested by Xho I and Apa I. So the recombined expression plasmid pGFP-P80 is achieved. Then it is sequenced, PCR and digested by the restriction enzymes Xho I and Apa I. The results give that pGFP-P80 is achieved successfully and the insert sites, direction and reading frame are all right. It builds the basis for expressing, purifying, gaining p80 protein from mammiferous cells.

将pMD-P80 分别经Xho I 和Apa I 双酶切和回收，然后与经过Xho I 和Apa I 酶解的真核表达载体pEGPF-C1 连接、转化，获得重组质粒，经PCR，Xho I 和Apa I 限制性酶切和序列测定，鉴定为真核表达质粒pEGFP-P80，并且目的基因的插入位置、方向和读码框完全正确，为在哺乳动物细胞中表达并分离纯化p80 蛋白奠定了基础。

The free radical scavenging effects of Meretrix meretrix hydrolysates prepared with papain,neutral protease and acid protease were investigated.

利用正交实验研究了文蛤蛋白分别经木瓜蛋白酶、中性蛋白酶、酸性蛋白酶作用后酶解物对羟自由基和超氧自由基的清除作用，优化了水解条件，并对最佳清除率下的三种酶解物进行SephadexG50凝胶柱分离，测定酶解物中抗氧化活性肽的分子量分布。

It's founded that Pistia stratiotes' soluble protein content,proline,peroxidase and catalase increased first and decreased afterwards with the augmenting of Cd2+ and Cr6+ concentration in the solution;With Cd2+ and Cr6+ concentration augmenting,SOD activity of Pistia Stratiotes increased first and decreased afterwards;But with Cd2+ Concentration augmenting, SOD Activity of Myriophyllum spicatum increased first and decreased afterwards; with Cr6+ concentration augmenting,SOD activity Myriophyllum spicatum droped at all times.

结果表明：随着水体Cd2+和Cr6+浓度的增加，大薸和穗状狐尾藻的可溶性蛋白含量、脯氨酸、叶绿素、丙二醛含量、硝酸还原酶活性、过氧化物酶活性、过氧化氢酶活性都是先升高后降低；随着Cd2+和Cr6+浓度的增加大薸超氧化物歧化酶活性先升后降；而穗状狐尾藻随着Cd2+浓度的增加SOD活性先升后降，随着Cr6+浓度的增加，SOD活性直线下降。

Results According to the treatment group, The total protein、albumin、globulin、the former albumin、serum glucose are higher than that of the control group; the Immunoglobulin (IgG、IgA、 IgM) are significantly higher than that of the control group, and the urea is significantly lower than that of the control group. Other indicators: total bilirubin、glutamic pyruvic transaminase、glutamic oxalacetic transaminase、alkaline phosphatase、creatinine、hemoglobin and the red blood cell count have no significant difference between the two groups.

结果 治疗组总蛋白、白蛋白、球蛋白、前白蛋白、血糖比对照组高；尿素比对照组明显减低；免疫球蛋白（IgG、IgA、IgM）比对照组明显升高；总胆红素、谷丙转氨酶、谷草转氨酶、碱性磷酸酶、肌酐、血红蛋白、红细胞计数等项，两组无明显改变。

Comparison from the Germany isolate, phocine distemper virus type Ⅱ(PDV-2) and vaccine strain, the major difference is the long signal peptide domain while the mature protein exhibit high identity, and all of the 13 serine residues, four potential asparagine glycosylation sites and two hydrophobic regions supposed to affect the fusion function are completely identical. These data supports the view that F protein is more conservative than H protein in CDV.

三、犬瘟热病毒中国分离株囊膜糖蛋白基因免疫小鼠诱发的抗体应答经一系列步骤将CDV-YZ0101株的两囊膜糖蛋白基因定向导入真核表达载体pcDNA3.1中，DNA测序和酶切分析筛选阳性重组质粒克隆pcDNA-H和pcDNA-F，以重组质粒DNA和脂质体共转染COS-7细胞，用间接免疫荧光试验证实转染的COS-7细胞的胞浆中分别表达了CDV的H和F蛋白。

The results show that with Cd2 + Stress Pistia stratiote large increase of the concentration of leaf tissue of the chlorophyll content decreased, while the MDA, soluble protein content, proline content, lipid membrane permeability, SOD, POD, CAT, nitrate reduction The first enzyme content was increased at different times and reached a peak after the downward trend.

结果显示，随着镉胁迫浓度的增加大薸叶组织内的叶绿素含量呈下降的趋势，而丙二醛、可溶性蛋白含量、脯氨酸含量、脂膜相对透性、超氧化物歧化酶、过氧化物酶、过氧化氢酶、硝酸还原酶的含量呈先升高并在不同的浓度达到高峰后再下降的趋势。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。