酶蛋白

The adenovirus plasmid was identified by PacI digestion and transfected into 293A cells to package a recombinant adenovirus which expressed the Fhit protein. Furthermore, the adenovirus rAd-Fhit was infected into colon cancer cells,and the expression of the ectogenic protein was detected by Western blotting. Finally, the proliferation of colon cancer cells was observed in adenovirus-infected cells by the MTT assay.

经PacI酶切鉴定正确后，将重组腺病毒质粒转染293A细胞获得表达Fhit蛋白的重组腺病毒rAd-Fhit，将获得的重组腺病毒感染结肠癌细胞，采用蛋白印迹法检测外源Fhit蛋白的表达，并进一步观察其对细胞增殖能力的影响。

Methods To determine the composition and activation of cerebroproptein hydrolysate by aminoacid analyzer,HPLC,SDS-PAG and succinate denydrogenase,to determine the therapeutic effectiveness of cerebrprotein hydrolysate by ordinary pressure oxytolerant experiment and paramnesia experiment.

用氨基酸分析仪、高效液相色谱仪、SDS凝胶电泳和琥珀酸脱氢酶法测定脑蛋白水解物的组成与活性。用常压耐缺氧实验和记忆获得障碍法测定脑蛋白水解物的疗效。结果：脑蛋白水解物的氨基酸与小分子肽的比例分别为 80 %与 2 0 %左右。

SW480 and SW620 cells were also used to study the gene regulation with high carbohydrate intake, where it was shown that during glycolysis eight genes, HK1 (nuclear gene encoding mitochondrial protein, transcript variant 1), GPI, GAPD (glyceraldehyde-3-phosphate dehydrogenase), PGK1 (phosphoglycerate kinase 1), PGK2 (phosphoglycerate kinase 2), ENO2 (enolase 2), PKM2 (pyruvate kinase, muscle, transcript variant 2) and GLUT1 (facilitated glucose transporter, member 1) are over-expressed. In our study, we also found that during hypoxia of cancer tissues the resulting up-regulation of HIF-2α(hypoxia-inducible factor 2, alpha subunit) would in turn up-regulate the GLU1 gene, further activating glycolysis.

另一方面，在高碳水化合物的调控研究上，吾人也利用SW480和SW620细胞株分析证实醣解水解活化过程中，其六碳醣激酶-1(HK1)、葡萄糖磷酸异构酶、3-磷酸甘油醛脱氢酶、磷酸甘油酸激酶-1(PGK1)、磷酸甘油酸激酶-2(PGK2)、烯醇酶-2(ENO2)、丙酮酸激酶-2(PKM2)以及葡萄糖输送蛋白-1(GLUT1)等共有8个基因是连续上升的过度表现；此外，吾人也发现当癌细胞在缺氧的组织中，其缺氧转录子-2α基因(HIF-2α)表现上升后，会活化GLU1基因上升，进一步活化醣解代谢的进行。

The activities of enzymes including Polyphenol Oxidase, Superoxide Dismutase, Catalase, Peroxidase and the contents of soluble protein, phenolics contents and varieties in the pericarp, sarcocarp and core of the pear fruits were measured.

以黄金梨、大香水、新梨七号为试材，研究了贮藏后梨褐变不同部位酚类物质含量和种类、多酚氧化酶、超氧化物歧化酶、过氧化氢酶、过氧化物酶的活性及可溶性蛋白的关系。

With western blot analysis and DNA polymerase chain reaction-single strand conformation polymorphism assay,protein product of Rb gene and mutation in exon 5～8 of P_(53) gene were examined in 17 cases of human testicular seminoma.

采用Western印迹和DNA聚合酶链反应－单股构造多态性分析法对17例人睾丸精原细胞瘤组织标本的抗癌基因Rb表达的蛋白产物和P_（53）基因外显子5～8的突变进行检测，发现有3例标本的Rb基因表达的蛋白产物缺失，有4例标本的P_（53）基因有点突变，对照的正常人睾丸组织和视网膜组织均呈Rb基因蛋白产物表达阳性，对照的正常人睾丸组织中未发现P_（53）基因突变。

The apoB gene polymorphism on Xba I site of 53 6 unrelated Chinese Han people,aged 25～64 years,was analyzed using PCR and Re striction Fragment Length Polymorphismmethod;serum apoA I,apoB,total chole s terol,triglyceride,HDL cholesterolwere measured,and LDL choleste roland non-HDL cholesterolwere calculated.

采用聚 合酶链反应结合限制性片段长度多态性分析的方法，分析536名25～64岁的无血缘关系的汉族人的apoB基因Xba I位点多态性，并测定其血清apoA I、apoB、总胆固醇、甘油三酯、高密度脂蛋白胆固醇，并计算低密度脂蛋白胆固醇LDL-C）及非高密度脂蛋白胆固醇。

Serum level of TNF-α was measured by unsaturation assay; Serum level of IL-2、IL-6、 IL-10 were measured by radioimmunoassay.

采用非饱和法测定肿瘤坏死因子α的水平；采用放射免疫法测定白介素-2(IL-2)、白介素-6(IL-6)和白介素-10(IL-10)的水平；采用酶联免疫吸附法测定肌钙蛋白Ⅰ的水平；采用速率法测定丙氨酸转换酶的水平；采用肌氨酸氧化酶法测定肌酐的水平。

The hydrolysates of five proteins materials,namely fish meal,meat and bone meal,soybean meal,rapeseed meal and wheal gluten meal,were prepared with alcalase and their root-inhibiting activity to Indian dendranthema, Dendranthema indicum Des Moul was measured in Petri dish at different concentrations,i.e.0,0.5,1,2,5 mg/mL.

以鱼粉、肉骨粉、豆粕、菜籽粕及芡粉等几种天然蛋白原料为底物，以碱性蛋白酶为水解酶制备出天然蛋白原料的水解物，用培养皿生物分析法检测不同蛋白原料水解物在不同浓度(0.5、1、2、5 mg/mL)条件下对杂草野菊种子的生根抑制活性。

A new type of Ca~(2+) sensors unique to higher plants have been studied recently, they are the so-called CBLs for their similarity to B-subunit of yeast and animal calcineurin. Plant CBLs interact with and regulate the activity of a group of Ser/Thr protein kinases, referred to as CIPKs .

在高等植物体内存在一类类似于酵母和动物体内钙调磷酸酶B的蛋白，简称CBL，它的靶蛋白是一类具有丝氨酸/苏氨酸激酶活性的蛋白，被称为CIPK(CBL-interacting protein kinase)。

We detected fasting plasma glucose by glucose oxidase, fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology, tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index, that is, ISI=1/FPG×Fins.

血糖测定采用葡萄糖氧化酶法；胰岛素测定采用化学发光法；血脂（甘油三酯TG、高密度脂蛋白HDL-C）测定采用酶法；肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法；胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数，即ISI=1/FPG ×FIns。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。