酶蛋白

This review deals with the active site structure, reactivities and chemical mimics of the binuclear copper enzymes, particularly hemocyanin and tyrosinase .

本文综述了双核铜酶，特别是酪氨酸酶、血蓝蛋白的结构，键合氧、活化氧的性能及有关仿酶模型的研究进展。

She and her colleagues used the detergent SDS, which is usually used as denaturant to degrade protein, to activate hemocyanin. At certain high concentrations, instead of destroyomg the complex, it turns hemocyanin into an enzymatically active phenoloxidase.

她和她的同事用一种经常被用作蛋白质变性剂的十二烷基磺酸钠来活化血蓝蛋白，它在一定的高浓度下，增强血蓝蛋白酚氧化酶的活性而不会破坏血蓝蛋白复合物。

Use biochemical method to observe the effect of electro-acupuncture on AchE、SOD、MAO、NO、NOS of senile dementia AD rat's brain tissue; use immunohistochemistry technique to observe protein kinase expression of hippocampal; use optical and electron microscope to observe morphosis change of rat's brain tissue; use in situ hybridism technique to observe aspartate receptor mRNA expression of hippocampal; use water and electric maze experiment to observe rat escape latent period and mistake times, to understand it's learning and memory ability.

分别用生化方法观察电针对AD大鼠脑组织胆碱酯酶、超氧化物歧化酶、单胺氧化酶、一氧化氮、一氧化氮合酶的影响；用免疫组化方法观察海马组织蛋白激酶的表达；用光、电镜方法观察大鼠脑组织形态结构的变化；用原位杂交方法观察海马组织天门冬氨酸受体rnRNA的表达；用水迷宫和电迷宫实验观察大鼠逃避潜伏期和错误次数，以了解其学习记忆能力。

The result showed that twocDNA fragments which expressed at high level both in shoot and radicle representedthe gene encoding beta-D-glucosidase; one cDNA fragment expressed specifically inshoot represented the gene encoding mitochondria HSP60; most clones of MF12 andMF17 fragments respectively represented the chloroplast genes encoding prp22 andprp19 proteins which are two components of ribosomal small subunit; while thededuced amino acid sequence from each exceptional one clone was respectivelyhomologous to CDC5 proteins and vesicle-associated membrane proteins; otherthree cDNA fragments expressed preferentially in shoot had no homologue inGenBank.

结果发现2个在胚芽和胚根中表达量都很高的cDNA片段代表的是编码玉米β-D-葡糖苷酶的基因；一个在胚芽中表达而在胚根中不表达的片段代表的是编码线粒体分子伴侣HSP60的基因；片段MFl2的大部分克隆测序结果是与叶绿体基因组中编码核糖体小亚基蛋白prp22的基因同源，但其中有一个克隆测定的cDNA片段序列推测的氨基酸序列与CDC5家族成员有较高的同源性；片段MF17的大部分克隆测序结果与叶绿体基因组中编码核糖体蛋白prp19的基因同源，而有一克隆测定的cDNA片段序列推测的氨基酸序列与参与信号转导的膜结合蛋白VAP27和VAMP有较高的同源性；另有3个优先在胚芽中表达的cDNA片段未查询到同源序列。

The clathrin triskelions assenble into a basket-like convex framework that causes the membrane to invaginate clathrin-coated vesicles migrate into the cell where the clathrin coats are lost before delivering their contents to the lysosomes.

网格蛋白三脚复合体装配成篮状凸起懂得框架，使得膜在该处内陷，最终出芽而形成小泡。而在胞吞作用中，这些网格蛋白包被的小泡转移到细胞中，此处网格蛋白外被在小泡的内含物转移到溶酶体之前即行消失。

In our test, the mutation in S1 gene seemed notthe major reasons responsible for IBV tropism switch. 3: Relationship between HA activity and cross-species infection of IBV After treated with lecithinase C, the strains M41, H52, H120 and Gray, but notHolte, Connecticut and M52-19, could haemagglutinate 0.5% chicken erythrocytes.

唾液酸糖蛋白在IBV跨种感染过程中的作用某些冠状病毒可以利用细胞表面的唾液酸糖蛋白作为进入细胞的受体，而神经氨酸酶可以降解细胞表面的唾液酸糖蛋白，这样就会降低病毒感染的几率。

BACKGROUND AND AIM: To purify and investigate the enzyme kinetic properties of rabbit plasma semicarbazide_sensitive amine oxidases, using methylamine as substrate.

背景与目的：血浆中含有氨基脲敏感型胺氧化酶。本文研究以甲胺为底物的兔血浆中具有SSAO酶活性的蛋白组分的酶动力学参数。

Methods HPV16 DNA in fection rate was tested in cervical carcinoma and normal cervical tissues by polymerase chain reactiontechnique.

采用聚合酶链反应检测宫颈癌及正常宫颈组织中HPV16感染率，用蛋白印迹技术对HPV16DNA阳性的宫颈癌组织中是否存在HPV16 E7蛋白和R6蛋白 E2F 1形成的复合物进行检测。

Methods The expression of CD147 and MMP-2 proteins were detected by immunohistochemical method in 46 cases of gliomas, and compared with those in 14 cases of normal brain tissues. The correlation between them was analyzed.

应用免疫组织化学链霉素抗生物素蛋白－过氧化酶连接法，检测46例胶质瘤瘤组织中CD147蛋白及MMP-2蛋白的表达，分别与正常脑组织14例进行对照。

Give birth to content and food engineering according to university of industry of project technology chief, Dalian courtyard dean Zhu Bei osmund professor introduction, this project developed microtherm vacuum to permeate first flavor to style of technology, stage warms up and cool antiseptic processing technique, in the treatment that uses at eating abalone product namely, solved original feed the difficult problem of technology of not easy pilot such as abalone product appearance, mouthfeel, aroma, color namely, built the manufacturing technology system that feeds abalone namely, to develop other ocean shellfish fed a product to lay good foundation namely; Use first from dissolve enzymatic with outside polysaccharide of abalone of extraction of method of source enzymatic compound, raised the recovery rate of polysaccharide greatly; Use enzymatic law and the method that Sevag law photograph combines, solved effectively take off the difficult problem of miscellaneous albumen in dividing abalone polysaccharide, raised polysaccharide purity, maintained polysaccharide active, the technical system of the preparation that built abalone polysaccharide, detached, rarefied, analysis, draw lessons from goodly to developed polysaccharide of other and marine animal to offer.

据项目技术负责人、大连工业大学生物与食品工程学院院长朱蓓薇教授介绍，该项目首次开发了低温真空渗透调味技术、阶段式升暖和冷却杀菌加工技术，并应用于即食鲍鱼产品的加工中，解决了原有即食鲍鱼产品外形、口感、香气、颜色等不易控制的技术难题，建立了即食鲍鱼的生产技术体系，为开发其他海洋贝类即食产品奠定了良好的基础；首次采用自溶酶与外源酶复合的方法提取鲍鱼多糖，大大提高了多糖的提取率；采用酶法与Sevag法相结合的方法，有效地解决了脱除鲍鱼多糖中杂蛋白的难题，提高了多糖纯度，并保持了多糖活性，建立起了鲍鱼多糖的制备、分离、纯化、分析的技术体系，为开发其他海洋动物多糖提供了良好的借鉴。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。