酶蛋白

In vitro SUMO modification of the BRCA1/BARD1 heterodimer greatly increases its ligase activity, identifying it as a SUMO-regulated ubiquitin ligase. Further, PIAS SUMO ligases are required for complete accumulation of double-stranded DNA damage-repair proteins subsequent to RNF8 accrual, and for proficient double-strand break repair.

Galenty等人发现，两个连接酶（PIAS1 和 PIAS4）在DNA双链断裂处将各种不同的SUMO蛋白添加到DNA修复蛋白上，而SUMO蛋白则是泛素随后修饰这些蛋白中的其中一些所必需的。

IGF-1 concentration in serum was measured with RIA. Protein concentration in urine was measured with ELASE. Urinary protein excreted in one day was measured with ponceau S. Total protein and albumin level in serum were measured with automatic biochemistry appliance.

以放射免疫法测血清IGF-1水平，用酶联免疫吸附试验测尿微量白蛋白浓度，S-丽春红测24h尿蛋白定量，全自动生化仪测量血清总蛋白和白蛋白。

In order to establish a sensitive immuno-PCR assay for detecting serai anti-P53antibodies, investigate the relation of anti-P53 antibodies in patients with breast carcinoma to P53 protein expressions in tissue and clinical significances and afford a practical tool in diagnosis of breast carcinoma clinically. SaHIgG was coupled to PNCa DNA by chemical cross-linker PEI and SaHIgG-DNA probe was constructed.

本研究建立了血清抗P53蛋白抗体免疫聚合酶链反应(Immuno-polymerase chain reaction，免疫PCR)检测方法，探讨了乳腺癌患者血清中抗P53蛋白抗体与组织中P53蛋白表达之间的关系及临床意义，旨在为临床乳腺癌P53蛋白检测提供实用的替代工具。

Cytokeratin, vimentin, alkaline phosphatase,α-actin,Ⅷ Ag, fibronectin and desmine were determined using the third generation cells. The ultrastructure were examinated by the transmitted and scanning electronic microscope.

取第三代细胞检测细胞角蛋白、波形蛋白、碱性磷酸酶、结蛋白、Ⅷ因子、α-肌动蛋白的表达，应用扫描电镜和透射电镜观察细胞的超微结构。

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具，分别以人丁酰胆碱酯酶(1p0i：A)和果蝇乙酰胆碱酯酶(1d×4：A)的蛋白晶体结构为模板，对嗜虫书虱2个AChE的三维结构进行同源建模，并在三维结构中发现了AChE的酶解活性位点，证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少，在GenBank中没有可用作内参基因的序列，因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号：FJ041117)，该片段长度为822 bp，编码273个氨基酸残基，同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

The influence of three kinds of heavy metal on the activities of peroxidase, superoxide dismutase,catalase and the contents of malondialdehyde, soluble protein and chlorophyll of Lemna minor was investigated.

对水体中主要的3种重金属对青萍抗氧化酶系统的几种酶，包括过氧化物酶、超氧化物歧化酶、过氧化氢酶的活性，丙二醛的含量，可溶性蛋白含量及叶绿体色素含量等指标的影响进行了研究。

The paper discussed how docosahexaenoic acid regulated transcription expression of lipogenic and lipolytic genes, which included PPARγ(peroxisome proliferators activated receptor γ), SREBP-1c (sterol regulatory element binding protein-1c), FAS, HSL (hormone-sensitive lipase) and TGH.

探讨二十二碳六烯酸对小鼠脂肪组织和肝脏生脂相关基因过氧化物酶增殖物激活受体γ、固醇调节元件结合蛋白－1c基因(SREBP-1c)、脂肪酸合成酶基因，及脂解相关基因激素敏感脂酶基因和甘油三酯水解酶时序表达的影响。

In lung cancer tissues, benign phymatoid lesions, and adjacent non cancer lung tissues, telomerase activity was determined by telomeric repeat amplification protocol assay, and the expression of human telomerase RNA component, human telomerase reverse transcriptase, and human telomerase associated protein(hTEP1) mRNA were detected with reverse transcriptase PCR. The results were compared with the histological types, degree of differentiation, and TNM stage of lung cancer.

用 TRAP法检测端粒酶活性，用逆转录 PCR法检测肺癌组织、肺部良性瘤样病变和病灶旁非癌肺组织的 RNA成分（ human telomerase RNA component，hTR）、端粒酶逆转录酶（ human telomerase reverse transcriptase， hTERT）、端粒酶相关蛋白 1（ human telomerase associated protein， hTEP1）各亚基的 mRNA表达，其结果与肺癌的组织类型、分化程度及 TNM分期进行了比较。

The shorter variant mHemk2 is 1696 bp in length encoding a putative mouse protein of 14.7 kDa, consisting of 138 amino acid residues.In protein data bank annotations, Hemk has previously been classed among methyltransferases methylating N6-adenine or N4-cytidine in DNA because of the presence of S-adenosyl-L-methionine binding motif and of an NPPY motif in the protein.

在蛋白数据库的注释中，由于在Hemk蛋白中存在S-腺苷-L-甲硫氨酸结合基序和一个NPPY基序，因此，在蛋白库的说明中已经将Hemk蛋白归类于能甲基化DNA中N6-腺嘌呤或N4-胞嘧啶的甲基转移酶。

The method has thyroid transcribe factor to specimen of 12 SHL tissue - antigen of 1(TTF-1), epithelial film , CK7, CK20, dash forward the albumen that touch bubble , undee albumen , flowing flesh actin , placental alkalescent phosphoric acid is enzymatic , be addicted to bead albumen, among them observation of 10 lens that make report.

方法对12例SHL组织标本进行甲状腺转录因子-1(TTF-1)、上皮膜抗原、CK7、CK20、突触泡蛋白、波形蛋白、平滑肌肌动蛋白、胎盘碱性磷酸酶、嗜铬粒蛋白、CD34的SP免疫组织化学法标记，其中10例做电镜观察。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。