酶蛋白

Ratio of enzyme to substrate, temperature, and hydrolysis time for the production of antioxidant peptides were analyzed with Response surface methodology in which reducibility and superoxide anion radical capturing rate was response value to build a predictive model for the enzymolysis of chickpea protein and the optimization of the enzymolysis technology parameters.

为优化Alcalase蛋白酶酶解鹰嘴豆蛋白制备抗氧化肽的工艺条件，采用响应面分析法（response surface methodology，简称RSM），以还原能力、超氧阴离子捕获率为响应值，研究了酶与底物的比值、酶解温度和酶解时间对制备抗氧化肽工艺的影响。

Methods: We divide the rats into 5 groups in random, with Morris method, detect the change of some active oxygen radicals such as super-oxide demitasse, Nitric Oxide Synthase, malondialdehyde etc. Also, we detect the change of cholinesterase, Monoamine Oxidize; using HE,PAS staining, immuno-histochemical technique, detect hippocampus CA1 cerebral pathology slices optical density value of NOS, CHE and lipofuscin of neural cell; detecting apoptosis rate and the expression of P53 , which are neuronal apoptosis related genes.

大鼠随机分为5组，采用"Morris水迷宫"法观察脑缺血性模型大鼠学习记忆功能，检测超氧化物歧化酶、一氧化氮合酶、丙二醛等自由基代谢异常的改变，检测胆碱酯酶、单胺氧化酶等老化相关酶的变化、脑组织神经细胞免疫组织化学技术、检查海马CA1区脑组织的病理切片，检测NOS、CHE光密度值；流式细胞技术：检测细胞凋亡率，及促细胞凋亡基因、P_(53)蛋白表达；病理切片采用HE染色、PAS染色及免疫组化法检查，检测对大鼠海马神经元细胞一般病理变化及脑细胞内脂褐含量。

To better understand the principles of enzymatic unhairing, changes of contents of protein, hydro-proline and glucose in depilatory liquors were determined in Lowery method, DMBA method and Sulfuric Acid-Anthrone method, respectively, during bath dehairing procedure of 2709 proteinase and A.

为更好地理解酶脱毛的机理，本文用Lowery法、对二甲胺基苯甲醛法和硫酸－蒽酮法分别测定了2709酶和A.S1398酶有温有浴酶脱毛过程溶液中的总蛋白、羟脯氨酸及糖含量的变化。

By releasing the cytosol proteins with Digitonin and centrifugation technology, we divided the J6-2 cell proteins into the cytosol proteins and the particulate-conjugated proteins, and then using SDS-PAGE, Western blotting and Enzyme-linked immunosorbent stain combined with computer quantiscan technology, we investigated the effect of GM3 on PLC1 translocation.

本文采用洋地黄皂甙增加细胞膜渗透性，释放胞液蛋白，通过离心将细胞蛋白分为胞液蛋白和颗粒结合蛋白两部分，然后采用SDS-PAGE，Western blot及酶联免疫显色技术，并结合计算机扫描半定量技术，检测了GM3处理前后PLC1在细胞内的转位情况。

Methods level of telomerase activation and PCNA expression were detected in 80 cases of breast cancer,36 cases of side-cancer tissues and 10 cases of lacteal gland benign lesion using TRAP-PCR-ELISA and S-P immunohistochemistry technique.

采用端粒重复序列扩增聚合酶链反应-酶联免疫吸附测定法(TRAP-PCR-ELISA)和免疫组织化学链霉素生物蛋白过氧化物酶法对80例乳腺癌组织、36例乳腺癌相应癌旁组织和10例乳腺良性病变的端粒酶活性及增殖细胞核抗原进行检测。

Methods The level of telomerase activation and PCNA expression were detected in 80 cases of breast cancer,36 cases of side-cancer tissues and 10 cases of lacteal gland benign lesion using TRAP-PCR-ELISA and S-P immunohistochemistry technique.

采用端粒重复序列扩增聚合酶链反应-酶联免疫吸附测定法(TRAP-PCR-ELISA)和免疫组织化学链霉素生物蛋白过氧化物酶法对80例乳腺癌组织、36例乳腺癌相应癌旁组织和10例乳腺良性病变的端粒酶活性及增殖细胞核抗原进行检测。

Methods The level of telomerase activation and PCN A expression were detected in 80 cases of breast cancer,36 cases of side-cancer tissues and 10 cases of lacteal gland benign lesion using TRAP-PCR-ELISA and S-P immunohistochemistry technique.

采用端粒重复序列扩增聚合酶链反应-酶联免疫吸附测定法(TRAP-PCR-ELISA)和免疫组织化学链霉素生物蛋白过氧化物酶法对80例乳腺癌组织、36例乳腺癌相应癌旁组织和10例乳腺良性病变的端粒酶活性及增殖细胞核抗原进行检测。

Ordor perception of insects is a large and complex system, involving many kinds of proteins such as odorant binding proteins, odorant receptor and odorantdegrading esterase etc.

昆虫对外界气味的感受作用是一个庞大而复杂的体系，多种蛋白参与了这一过程。其中包括气味结合蛋白，气味结合蛋白受体，气味降解酶等多种蛋白。

RESULTS: In vitro expanded UCMSCs constitutively expressed markers of hepatic lineage, including albumin, alpha-fetoprotein, cytokeratin-19, connexin-32, and dipeptidyl peptidase IV.

结果：体外扩增的UCMSCs 组成性表达肝细胞系的标记物，包括白蛋白，甲胎蛋白，细胞角蛋白19片段，连接蛋白-32和二肽基肽酶IV。

PIAS proteins play roles mainly through two mechanisms: acting as SUMO E3 ligases to promote modifications of some transcriptional factors and cofactors, especially sumoylation, and to regulate their transcriptional activation; acting as structural proteins to offer platforms for protein-protein interactions and to promote the abstraction and recruitment of other regulators in the cellular signal pathway complexes or gene transcriptional complexes, companied by the subnuclear location of target proteins.

PIAS蛋白的调控机制主要有两种：一种是通过其自身所具有的SUMO(small ubiquitin-related modifiers)E3连接酶活性，促进对一些转录因子、转录辅因子的化学修饰，尤其是SUMO化修饰，从而调控它们的转录活性；另一种是作为构架蛋白，为蛋白质之间的相互作用提供平台，促进细胞信号通路复合物或基因转录复合物中其它调节蛋白的去除和募集，并涉及到靶蛋白的亚核定位。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。