酶蛋白

The diets of test group 1, 2 and control group included in 0.5% enzymatic casein (small peptides above 80% and average length of peptides is 2.98), 0.5% acidify hydrolyzed casein(free amino acids above 80%) and 0.5% casein.

试验期56天。结果表明：添加O.5％酶解酪蛋白组草鱼其相对生长率、净蛋白沉积率、饲料系数和血浆中镁含量与小肽总量均显著高于添加0.5％酪蛋白组和0.5％酸解酪蛋白组(p＜0.05)。

398 Neutral protease,pepsin,papain and bromelin were utilized to hydrolyze the head of Penaeus monodon .

利用AS1.398中性蛋白酶、胃蛋白酶、菠萝蛋白酶及木瓜蛋白酶对斑节对虾头蛋白进行酶水解试验，从中选出了优选试验用酶，并在此基础上对优选酶的最适酶解工艺条件进行了探讨。

Results as bellows: AtSIRT1 was located in Mitochondrial as hSIRT4 of human, and maybe take part in respiration and electron transformation chain, AtSIRT2 was located in nucleolus as hSIRT6 of human, maybe play important role in extend lifespan;mutation in AtSIRT1 leaded to cotyledon of plant turn to yellow and caused short life span. Mutation in AtSIRT2 could make the color of leaf turn to purple and accumulate a lot of anthocyanin;Sirtinol, a inhibitor of SIRT which did not cause the same model of the mutation of AtSIRT1 and AtSIRT2 indicated that the mechanism of Sirtinol was different from other organism;the structure of AtSIRT1 and AtSIRT2 were similar to other known Sir2, which indicated that they maybe have the same function;AtSIRT2 was overexpressed and its activity was detected.

结果表明，1，拟南芥AtSIRT1与人的同源蛋白hSIRT4相同，定位于线粒体，可能参与呼吸作用和电子传递，SIRT2与人的同源蛋白hSIRT6相同，定位于细胞核，可能同它的功能类似，在延缓衰老及调节细胞寿命方面起作用。2，AtSIRT1突变，可引起幼苗和植株的子叶变黄和早衰；AtSIRT2突变，可引起叶片发紫，沉积大量花青素。3，SIRT蛋白的抑制剂Sirtinol不能表型模写AtSIRT1和AtSIRT2突变体，说明Sirtinol在拟南芥中的作用机制不同于其他生物。4，AtSIRT1和AtSIRT2蛋白质结构预测表明与已知的Sir2蛋白相似，揭示其功能的相似性。5，在大肠杆菌中过量表达了其中一个基因(AtSIRT2)，可体外检测其酶学活性，进一步证明其功能。

In order to optimize enzymolysis technology for production of antioxidant peptides from chickpea protein, effect of ratio of enzyme to substrate, enzymolysis temperature, and hydrolysis time on the technology in which reducibility and superoxide anion radical capturing rate were taken as response values was analyzed with response surface methodology.

为优化Alcalase蛋白酶酶解鹰嘴豆蛋白制备抗氧化肽的工艺条件，采用响应面分析法，以还原能力、超氧阴离子捕获率为响应值，研究了酶与底物的比值、酶解温度和酶解时间对制备抗氧化肽工艺的影响。

The reason why only when wool is pretreated with hydrogen peroxide can protease SZ confer wool excellent antifelting property is that the pretreatment can remove some lipid on epicuticle, thus the protease SZ has the possibility to digest the protein of epicuticle for no lipid's obstruction.

SZ酶之所以需在双氧水氧化预处理的条件下才能使羊毛织物获得优良的防毡缩性能，是因为双氧水预处理在打开羊毛蛋白部分交联的同时，也可去除羊毛鳞片表面部分类脂，为SZ酶进攻羊毛鳞片表层蛋白提供了可能。

The uptake of 〓I-OxLDL in adipocytes was determined by gamma ray counter and normalized to protein concentration of each sample, was expressed as nanograms of 〓I-OxLDL uptake per milligram of cell protein. Reverse transcription polymerase chain reaction was used to evaluate PPARγ and CD36 mRNA expressions.

采用酶联免疫吸附法检测血浆IL-6以及脂肪细胞分泌IL-6的水平；采用gamma射线计数仪来检测脂肪细胞对〓I-OxLDL的特异性摄取情况，采用改良Lowry法进行细胞蛋白浓度测定，细胞对〓I-OxLDL的特异性摄取量以ng／mg细胞蛋白表示；采用逆转录聚合酶链式反应来检测PPAR γ和CD36 mRNA在脂肪细胞的表达情况。

The biomass production of grafted seedlings, activities of antioxidant enzymes and contents of proline and soluble protein in leaves of grafted seedlings were significantly higher than those of own-root seedlings, while O2(superscript -) production rate, contents of H2O2 and MDA in leaves of grafted seedlings were significantly lower than those of own-root seedlings under Ca(NO3)2 stress.

结果表明，Ca(NO3)2胁迫明显抑制植株生长，显著提高植株抗氧化酶活性，显著增加植株O2生成速率以及H2O2、MDA、脯氨酸和可溶性蛋白含量，但胁迫后嫁接苗的生物量显著高于自根苗，抗氧化酶活性、脯氨酸和可溶性蛋白含量均显著高于自根苗，而O2生成速率、H2O2和MDA含量则显著低于自根苗。

The cytochrome P450 is a superfamily of hemoproteins that are the terminal oxidases of the mixed function oxidase system.

细胞色素P450是一血红素蛋白的超家族，这些血红素蛋白组成混合功能氧化酶系统中的末位氧化酶。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明：(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞；正常成年男性睾丸组织中同样存在COX-2表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞；(2)服用特异性COX-2酶抑制剂rofecoxib 2周后，实验组大鼠睾丸组织内睾酮的含量减少，为正常对照组的50％；持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平；COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩，生精紊乱，持续用药4周时影响最明显；(3)注射特异性Leydig细胞杀灭剂EDS 2周后，实验组大鼠睾丸组织内睾酮浓度降至极低水平时，COX-2的蛋白和基因表达水平也显著低于正常空白对照组（P.005）；使用EDS后第4周，睾丸组织中的睾酮浓度逐渐回升，同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平；使用EDS后4周开始给予外源性睾酮，6周和8周时COX-2表达水平的绝对值虽有提高，但与正常大鼠空白对照组比较并无显著性差异，说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

Thus, the "yellow enzyme" is isolated as a conjugated protein in which the non-amino acid moiety is riboflavin phosphate, while catalase is an iron-porphyrin-containing protein

比如说，分离出来的结合蛋白-&黄酶&便是一例，它的非氨基酸部分是磷酸核黄素。而过氧化氢酶则是一种含卟啉铁的蛋白。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。