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At the same time, adding thymoquinone to pancreatic cancer cells reduced the production and activity of enzymes called histone deacetylases, which remove the acetyl groups from the histone proteins, halting the gene transcription process.

同时,添加百里香醌到胰腺癌细胞能够减少细胞产物并且激活组蛋白脱乙酰基酶,这种酶将从组蛋白中移除乙酰基基团,从而阻止了基因的转录过程。

To elucidate the possible mechanism of differentiation and /or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor, the level of acetylated histone H3 was detected by Western blot, p21~ WAF1expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin,TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制,利用Westernblot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21WAF1表达量的改变。

To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor , the level of acetylated histone H3 was detected by Western blot, p21(superscript WAF1) expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin, TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制,利用Western blot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21(上标 WAF1)表达量的改变。

The two NS5B proteins showed the same RdRp activity changes to these three templates: they had the highest activity using 3'terminuses from HCV genotype 2a negative RNA strand as template and the lowest activity using 3'terminuses of HCV genotype 1b negative RNA strand as template.

两种NS5B蛋白对HCV 1a、1b和2a型负链3'末端RNA模板表现出相同的活性差异,两种蛋白均对2a型负链3'末端RNA模板表现出最高的聚合酶活性,对1b型负链3'末端RNA模板表现出最低的酶活性。

Using tamato seedlings as experimental material, we examined the adaptive changes of the proton translocating pumps and protein components in vacuolar membranes of plant during phosphate starvation.

本项目以番茄为材料,对磷饥饿时植物液泡膜质子ATP酶和焦磷酸酶活性以及液泡膜蛋白组分的适应性变化进行研究,以探明磷饥饿时植物液泡膜质子泵活性的变化与Pi跨液泡膜运转的关系,并了解磷饥饿时植物液泡膜是否产生有特异性诱导蛋白及其可能的功能。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型,取健康大鼠每日灌服不同剂量的鲜姜有效部位(200,400,800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1),取含药血清作为受试药物,用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力;逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平;酶联免疫吸附法测定MCP-1蛋白表达水平。

These enzymes are incapable of breaking down or digesting wool,hair or silk fibres which are to them indigestible protein.

这些酶不会降解毛类,毛发及丝类纤维。这些蛋白对于这类酶来说是不可降解的蛋白。

As the only phosphatase regulated by Calmodulin, calcineurin plays an important role in psycological function.

钙调蛋白磷酸酶是唯一一个受钙调素调节的蛋白磷酸酶,有着重要的生理功能作用,然而它的CaM结合区及自抑制区的结构信息却长期处于未知状态。

The IWFs were purified by (NH4)2SO4 precipitation fractionation, and then applied to Sephadex G-75 column. The column eluates were collected separately according to A280 nm and injected into the health wheat leaves to examine the eliciting activities by PAL, PO assays.

IWF经硫酸铵粗沉淀后,将蛋白沉淀用重蒸水重新溶解后进行凝胶过滤柱层析,以280nm紫外吸收检测、收集不同的蛋白峰,分别注射到健康的Thatcher叶片中,以苯丙氨酸解氨酶(phenylalanine ammonialyase,PAL)、过氧化物酶(peroxidase,PO)防卫反应作为评价诱导活性的指标,检测活性成分在各部分中的分布和诱导活性的强弱。

Combined with the test of All〓wden reaction and observation with SEM, the data have proved that protease SZ can digest the protein of epicuticle and then hydrolyzed the cuticle of wool, while the other proteases have no this distinctive effects, although they can confer wool bigger weight loss too. As protease SZ can digest the epicuticle and cuticle, the wool can be confered excellent antifelting property with low tenacity and elongation loss.

本文结合羊毛的阿尔瓦登反应和电镜观察,证明了绝大部分酶制剂由于不具有对羊毛鳞片表层蛋白的水解特性,因而处理后的羊毛不具有良好的防毡缩性能,而SZ酶却可对羊毛鳞片表层蛋白进行水解,进而可以对羊毛的鳞片进行有效的减量改性,使羊毛获得优良的防毡缩性能。

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