酶蛋白

Animal models were worked out by injecting granule solution into the lung of mice through trachea to study pulmonary lesions in mice. All mice were intratracheally instilled every three days. At the tenth day, mice were sacrificed and. Lung organ coefficient, albumin, total protein, acid phosphatase and Alkaline phosphatase were measured.

为研究纳米二氧化钛对小鼠肺部的损伤，将小鼠采用非暴露式气管内注入法注入颗粒悬液建立动物模型，每隔2d染毒一次，在第10d处死小鼠，测定肺脏器系数及肺部组织中白蛋白、总蛋白、酸性磷酸酶和碱性磷酸酶的含量。

Schistosoma japonica antigen cDNA clones were identified by lysogenic expression,flat lyiic method and PCR amplification. All 8 positive clones immunologically screened could be expressed in E- coli in the form of fusion proteins with the molecular weight being about 140 to 150 kDa. The positive cDNA genes were digested by restriction endonuclease EcoRI,then the agarose gel electrophoresis revealed the size of them being 700 to 900 bp.

利用融源表达、平板裂解法和PCR扩增三种不同方法分别对日本血吸虫抗原cDNA基因进行鉴定和分析，8个免疫筛选阳性克隆均能在大肠杆菌中以融合蛋白的形式表达，表达蛋白分子量为140～150kDa，抗原cDNA基因经限制性内切酶EcoRI酶解后，琼脂糖凝胶电泳显示其大小为700～900bp，PCR能扩增出特异性条带。

There are many kinds of proteolytic enzymes in digestive system of Macaca mulatta tcheliensis, and proteolytic enzymes likeness exists between the organs with similar structure and similar functions.

消化系统中蛋白水解酶的种类和数量比较多，且结构相似、功能相近的器官之间，蛋白水解酶的种类和性质相似。

Synergistic down|regulation of telomerase activity and hTERT mRNA expression by combination of retinoic acid and GM|CSF in human myeloblastic leukemia ML|1 cells.

端粒酶激活过程中受到严格调控的部分是其蛋白亚单位，是否呈现端粒酶活性，主要是由蛋白亚单位的表达与否来决定的[6，7]。

Under different initial total egg white lysozyme concentrations in urea solution, the refolding egg white lysozyme intermediates could be deduced to have a tendency to form a bimolecular intermediate aggregate, and this inference was further confirmed by their nonreducing SDS-PAGE and size exclusion chromatography.

当不同浓度蛋白溶菌酶在脲溶液中复性时，蛋白溶菌酶折叠中间体有形成二分子积聚体的趋势，这个推测得到了非还原电泳和体积排阻色谱结果的证实。

Objective: To confirm the nucleolar localization of telomerase-regulation associated protein-human N-acetyltransferase-like protein and its associated functions.

目的：探讨端粒酶调节相关蛋白－乙酰转移酶样蛋白核仁定位的分子基础及相关功能。

Results:There were no abdominal pain and fever,jaundice faded away gradually postoperation.Biliary drainage was 300ml-1000ml/d more or less.Colour of bile changed into normal by degrees.Intra peritoneal drainage was open and the colour was light bloody.Amount of drainage dropped off day after day.Whole blood cell test, serum glutamic pyruvic transaminase,serum glutamic oxalacetic transarninase,total serum protein,serum albumin and serum globulin returned to normal in first week postoperation.Serum bilirubin,serum glutamyltranspetidase and serum alkaline phosphatase declined gradually in first week postoperation.T-tube was clipped at twelfth day postoperation.T-tube Cholangiography was clear at 24~ day postoperation.

结果：术后无上腹部绞痛、高热，黄疸逐渐减退，每日引流胆汁300ml-1000ml不等，由淡黄色混浊、内有胆泥过渡到金黄色清亮的正常胆汁，腹腔引流通畅，引流液为淡红色，量从最初的200ml迅速过渡到10ml，血常规、血清谷丙转氨酶、谷草转氨酶、总蛋白、白蛋白、球蛋白等1周内均恢复正常，血清总胆红素、直接胆红素、间接胆红素、谷氨酰转肽酶、碱性磷酸酶逐渐下降，术后12d夹闭T管，术后24d T管造影提示左、右肝管及胆总管下端通畅，无狭窄。

This paper summarizes the research in elucidating the structure of acid invertase genes and proteins, regulation of gene expression by organ and developmental specificity, sugar, wounding, pathogen infection, stress and hormones, and regulation of enzyme activity by proteinous inhibitors.

本文综述了植物酸性转化酶基因及其蛋白结构、基因表达的器官和发育特异性以及糖、受伤、病原、胁迫和激素对基因表达的调节和蛋白抑制因子对酶活性的影响，并讨论了当前在该研究领域存在的问题。

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

Sequence analysis and homology alignment showed that MD1 had 93% similarity with matK in maize chloroplast genome, a gene encoding maturase included in type Ⅱ intron splicing of RNA transcript; MD2 had 99% similarity with gene PP2C, encoding serine / threonine protein phosphatase type 2C in extremely drought tolerant Sporobolus stapfianus; and MD3 had 99% similarity with rice gene encoding metacaspase, belonging to aspartic acid specific cysteine caspases.

序列分析和同源性比对表明，MD1与玉米叶绿体基因组中编码参与RNA转录本Ⅱ型内含子剪切的成熟酶的matK基因有93%的相似性，MD2与极端耐旱的Sporobolus stapfianus的丝氨酸／苏氨酸2C型蛋白磷酸酶基因PP2C的相似性达99%，MD3与属天冬氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase基因有99%的相似性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。