酶蛋白

It was demonstrated that the cloned plcB gene appeared to be 834 bp in length, entirely identical with that published in GenBank The fusion protein GST-tagged broad- range phospholipase C showed bioactivity and immunogenicity in lecithinase test and Western blotting assay respectively.

结果 克隆的plcB基因长834bp，与GenBank上公布的序列完全相同；表达的与GST标签蛋白融合的广谱磷脂酶C蛋白具有磷脂酶生物活性和免疫原性。

Nighty rats were randomly divided into the groups of control, myocardial infarction and doxycycline, which were given orally 48 hours before and 48 hours after MI with 30mg/Kg per day. Protein and mRNA extraction was done on left ventricular samples containing scar and myocardium together. Samples were assayed from 1 day to 4 weeks post-Mi. The activity of MMPs was measured by zymography and collagen amount by the method of chloramine T, the ratio of II III collagen was assessed by immunohistochemical stain. Protein and mRNA of MMP-2, 9, TIMP-1 were determined by Western-Blot and RT-PCR.

分为对照组、心梗组和治疗组(D组，术前两天至术后两天，口服Doxycycline，30mg／kg／天)，分别于术后第一天、术后一周、术后两周、术后四周取心肌组织，采用免疫组化测定胶原含量和Ⅰ／Ⅲ胶原比例，酶谱法测定心梗后MMP-2，9活性蛋白的表达规律，Western Blotting进一步确定酶谱法中所消化条带蛋白的属性，逆转录-聚合链反应法测定心梗后MMP-2，9和TIMP-1的mRNA的变化规律，运用Acuson Sequoia 512超声心动图机分别测定心梗组、治疗组在两周和四周时左室前壁、后壁厚度以及左心室舒张末内径和左室射血分数。

A zinc-finger protein specific to a gene is loaded with an enzyme called a nuclease, which will bind to the gene and turn it on or off.

锌指蛋白以一种叫做a核酸酶的酶为载体结合在特定的基因上并将该基因沉默或者激活（不同的锌指蛋白结合的基因不同）。

In this study, the optimum conditions of the pancreas extracted pancreatin from porcine blood and utilization of the pancreatin to hydrolyze the porcine blood protein were studied.

研究了猪新鲜胰脏提取胰酶以及胰酶粗提液降解猪血蛋白获取蛋白水解液的条件。

First of all, preparing lactoferrin antimicrobial peptide from the enzymatic hydrolysis of lactoferrin was studied. Through single factor experiment and response surface regression analysis of the hydrolysis process, the optimal parameters were: enzyme of pepsin, lactoferrin concentrations of 30 mg/mL, the ratio of pepsin and lactoferrin 1.5%, temperature 44℃, pH 2.4, hydrolysis time 2 h.

通过单因素试验，响应面回归分析得到酶解的最佳工艺为：采用胃蛋白酶为酶源，乳铁蛋白浓度为30 mg/mL，胃蛋白酶与乳铁蛋白的质量比为1.5%，温度44℃，pH 2.4，水解时间2 h。

Methods Fifty-three patients diagnosed as chronic hapatitis of C were treated by IFN and Ribavirin for at least 24 weeks. Serum HCV-RNA was detected by fluorescent quantitative PCR. Genotype was determined by Simmonds. Results There was no difference of HCV-RNA levels in different genotypes, as that of ApoB.

临床确诊为慢性丙型肝炎的53例患者，采用干扰素联合利巴韦林抗病毒治疗至少24周，Simmonds酶切分型方法进行HCV基因分型，荧光定量聚合酶链反应法定量检测HCV-RNA，全自动生化分析仪检测血清载脂蛋白B，对不同基因型患者血清HCV-RNA水平和载脂蛋白B的关系进行研究分析。

Aim To understand the features of lipoprotein lipase and its role in the distinctive lipid metabolism in tree shrew, which is considered to be an animal protected from atherosclerosis.

目的 了解不易感动脉粥样硬化动物树鼩的脂蛋白脂肪酶的结构和活性特点，并与其它种属比较，探讨脂蛋白脂肪酶在树鼩独特脂代谢中的作用。

LPL was expressed in many tissues in tree shrew, highly in cardiac muscle and adipose tissue. In despite of no expression in skeletal muscle, there was less-medium expression in liver, distinctly different from human LPL which was expressed in the contrary way in these two tissues.

树鼩脂蛋白脂肪酶的组织表达谱比较广泛，在心肌和脂肪组织中大量表达，但在骨骼肌中未检测到，却在肝脏中有中低水平表达，与人脂蛋白脂肪酶在这两种组织中的表达相反。

Alb, Che and PA were examined by end-point method, enzyme kinetics and immunologic projective turbidimetry method respectively.

血浆清蛋白为终点法、胆碱酯酶为酶动力学法、及前白蛋白免疫投射比浊法。

Histone acetyltransferases and deacetylases function antagonistically to control histone acetylation.

组蛋白的乙酰化在调节染色体结构和功能中起着重要的作用，而这种调节受组蛋白乙酰化酶和去乙酰化酶的共同调节。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。