酶蛋白

CA II and AQP5 expression levels were determined by immunofluorescence and immunoblotting, while CA activity and Na, K-ATPase activity by a colorimetric assay. Anhidrotic mice treated by topiramate did not differ from controls in average secretory coil diameter, CA II expression, CA activity and Na, K-ATPase activity.

应用免疫荧光和western blot技术发现：小鼠在托吡酯处理后泌汗减少，碳酸酐酶同功酶II型(carbonic anhydraseII，CAII)蛋白表达未出现显著变化，但汗腺组织胞膜的水通道蛋白-5(aquaporin-5，AQP5)表达量下调。

The enzymolysis effect of neutral protease, papain, pepsin, bromelin, flavourzyme and compound protease was studied respectively on the sleeve-fish protein.

探讨了中性蛋白酶、木瓜蛋白酶、胃蛋白酶、风味蛋白、酶菠萝蛋白酶以及复合蛋白酶对鱿鱼蛋白的酶解效果。

The effects of different conditions (such as the ratio of enzyme amount to protein, pH, reaction temperature and reaction time) on the polymerization by MTGase were also investigated. The optimal conditions for sodium caseinate polymerized by MTGase were obtained: 10～20U/g protein, pH6. 0～8. 0, near 50℃.

还研究了酶量、pH、反应温度以及反应时间等因素对MTGase聚合酪蛋白酸钠的影响，得出较佳的催化工艺条件：酶量为10～20U/g蛋白；pH6.0～8.0；最适温度约为50℃。

It is postulated that IP induces the release of endogenous protective molecules that interact with cognate receptors to activate PLC through mediation of the chincough virus sensitive proteins. PLC hydrolyzes membrane phospholipid and produces diacylglyccerol,that,by working together with calcium hydronium,translocates PKC from cytoplasm to cell membrane and activates PKC.

推测缺血预处理细胞保护作用机制是：缺血预处理引起内源性保护物质释放，作用于细胞膜上的相应受体，通过百日咳病毒敏感性G蛋白的介导而激活磷脂酶，磷脂酶水解膜磷脂，产生二酰基甘油(diacylglyccerol，DAG)，DAG和Ca2+协同作用于PKC，使其从胞浆转位到细胞膜并且被活化，PKC在膜上使底物蛋白磷酸化，引发一系列相关信号转导通路的激活，调控保护性基因的表达，从而提高细胞对缺血再灌注损伤的耐受性。

In this article, we studied some components in giant panda seminal plasma and their relationship with semen characteristics.The protein bands were separated by SDS-PAGE, platelet-activating factor acetylhydrolase activity and glutamic-aspartic aminotransferase activity were detected by ELASE and chymic colorimetry respectively. The results are as followed:Twelve protein bands in giant panda seminal plasma were identified by SDS-PAGE, the molecular weights were 74.9、59.6、 56.2、 54.7、 47.3、 38.2、 30.8、 23.8、 18.4、 16.8、14.0 and 13.4kDa respectively, six of them (74.9, 59.6, 56.2, 47.3, 30.8 and 13.4kDa) werepresent in all samples. However, there was no relationship was found between the abnormalprotein bands appeared in several samples and semen characteristics in our experiment.

本研究采用SDS-PAGE对大熊猫精浆蛋白电泳谱带特性进行分析，使用酶联免疫方法检测精浆血小板激活因子乙酰水解酶活性、化学比色法检测精浆谷草转氨酶活性，并初步探讨了这些物质与大熊猫精液质量的关系，结果显示：(1)大熊猫精浆SDS-PAGE电泳共分离得到12条蛋白谱带，其分子量分别为74.9kDa、59.6kDa、56.2kDa、54.7kDa、47.3kDa、38.2kDa、30.8kDa、23.8kDa、18.4kDa、16.8kDa、14.0kDa和13.4kDa，其中74.9kDa、59.6kDa、56.2kDa、47.3kDa、30.8kDa、13.4kDa谱带为所有个体大熊猫精浆样品所共有。

The pancreatic proterses trypsin,chymotrypsin and elastase are all derived from zymogen precursors by proteolytic activation.

胰腺的蛋白水解酶—胰蛋白酶、胰凝乳蛋白酶和弹性蛋白酶都从酶原前体衍生而来，即由蛋白水解而活化的。

Litopenaeus vannamei were continuously fed by basic diets containing three levels (1%, 0.5%, 0.1%) Ig-Guard respectively for 20 days, and the basic diets as blank control. Phenoloxidase, acid phosphatase, alkaline phosphatase, lysozyme activity of the serum and superoxide dismutase, catalase activity of the muscle, and protein concentration of the serum and the muscle were detected in the 5, 10, 15, 20 day.

采用连续投喂的方法，饲喂凡纳滨对虾 20 d，分别测定了第5，10，15，20 d血淋巴的酚氧化酶、溶菌酶、酸性磷酸酶、碱性磷酸酶及肌肉匀浆液的超氧化物歧化酶、过氧化氢酶等非特异性免疫因子活性，并对血清及肌肉匀浆液中蛋白进行定量。

METHODS: The subcutaneous adipose tissue was obtained from adult New Zealand rabbits under aseptic condition, and cultured in vitro with collagenase digestion. All cells were divided into 3 groups: in the BMP-2 group, cells were cultured with medium containing 0.1 g/L vitamin C, 10 mmol/Lβ-sodium glycerophosphate and 10μg/L BMP-2 for 10 minutes, followed by 4-14 days inoculation with density of 18×104 cells per pore. In the BMP-7 group, cells were cultured with BMP-7 with the same methods as BMP-2 group. The cells were cultured with simple culture medium in the control group.

无菌切取成年新西兰大白兔皮下脂肪，胶原酶消化法体外分离培养脂肪干细胞，分为3组：骨形成蛋白2组加入含0.1 g/L维生素C、10 mmol/L β-甘油磷酸钠、10 μg/L骨形成蛋白2的诱导培养基培育15 min，然后按18×104个细胞/孔接种，再培育4~14 d；骨形成蛋白7组培养方法基本相同，仅将骨形成蛋白2更换为骨形成蛋白7；对照组同法加入单纯培养基进行培育。

The total activity of EPs increased at first and then decreased during both senescences. The changes of endopeptidase isozymes in wheat leaf senescence were studied by using natural gradient-polyacrylamide gel electrophoresis co-polymerized with hemoglobin or gelatin in the gel.

主要研究内容如下： 1、小麦叶片衰老过程中内肽酶的变化蛋白质的降解需要蛋白水解酶，包括内肽酶和外肽酶的参与，叶衰老过程中发挥主要作用的是内肽酶。

Six senescence-associated endopeptidase isoenzymes were identified by using natural gradient-polyacrylamide gel electrophoresis co-polymerized with gelatin in the gel, five of which (EP1、EP2, EP4、EP5 and EP6) were only detected in senescing leaves.

另外，采用不同的内肽酶同工酶电泳方法研究中均发现自然衰老和暗诱导衰老过程中的内肽酶同工酶谱变化基本相似，因此暗诱导衰老可以作为一种研究叶片自然衰老过程中出现的蛋白水解酶的特性的模式。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。