酶蛋白

Experiment 4: Affection of Ginger Extraction on ATPase of Myocardium of Rabbit. Method Coomassie brilliant blue protein test-box and ATPase test-box were used to detect activities of Na〓-K〓-ATPase、Ca〓-ATPase and Mg〓-ATPase in the myocardium of rabbits, samples from the low-dosage, middle-dosage ginger group and 4-Retro-decoction group of the above"Experiment 3". A extra control group (10 rabbits) was established in this experiment.

干姜提取物对兔心肌ATP酶活性的影响方法采用考马斯亮兰蛋白测试合和ATP酶测试合对兔心肌Na〓-K〓ATP酶、Ca〓-ATP酶和Mg〓-ATP酶的活性进行检测，标本来源于上述"实验3"的低、中剂量干姜组和四逆汤组，并另设心衰对照组（10只兔）。

Potassium, natrium determines it is good that use ion chooses electrode indirect method it is better also with indirect method to determine at calcium of direct way;, it is next occasionally phosphor of; of law of azotic arsenic Ⅲ determines use phosphor molybdic acid is ultraviolet law outcome is better it is better with result of law of personal candy kinase that; blood sugar determines; urea determines use Niao enzymatic ultraviolet anhydride of better; flesh uses rate law result enzymatic law outcome is better; albumin determines, it is vanadium acid next alkalescent phosphoric acid of oxidation law; is enzymatic determine outcome of fluid of use AMP amortize is better; amylase determines law of thing of enzymatic standard background and iodic colorimetric law coefficient of variation all bigger, former a bit small, latter of percent of pass is tallish, each lab can try to choose according to his condition.

结论钾、钠测定使用离子选择电极间接法好于直接法；钙测定用间接法也较好，其次是偶氮砷Ⅲ法；磷测定使用磷钼酸紫外法结果较好；血糖测定用己糖激酶法结果较好；尿素测定用脲酶紫外速率法结果较好；肌酐用酶法结果较好；白蛋白测定用溴甲酚绿法较好；胆红素测定用酶法结果好，其次是钒酸氧化法；碱性磷酸酶测定使用AMP缓冲液结果较好；淀粉酶测定酶法底物法和碘比色法变异系数均较大，前者稍小，合格率后者稍高，各实验室可根据自己的条件加以选用。

The histochemistry staining of cytokeratin, vimentin, alkaline phosphatase were positive and that of α-actin,Ⅷ Ag, fibronectin and desmine were negative.

细胞角蛋白、波形蛋白和碱性磷酸酶染色阳性，α-肌动蛋白、Ⅷ因子相关抗原、纤维连接蛋白和结蛋白染色阴性。

PON has been shown to be closely associated with lipometabolism by protecting LDL from oxidative modification,decreasing level of ox-LDL and destroying lysophospholipid of ox-LDL .

对氧磷酶由355个氨基酸组成，它与高密度脂蛋白中的载脂蛋白结合，能保护低密度脂蛋白免受氧化修饰，降低体内氧化型低密度脂蛋白水平，并且能破坏氧化型低密度脂蛋白中的溶血磷脂，与脂代谢有密切关系。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Studied the stachydrine dealing with the Oncomelania hupensis's esterase、the glycogen and the total protein. The result showed when dealt with the Oncomelania hupensis for 1~2 days, the figures of enzyme are higher than the compared group; when dealt for 3~4 days, the figures of enzyme reached the highest level; when dealt for 5 days, the figures of enzyme remarkably reduced.

用益母草中水苏碱处理钉螺导致其酯酶同工酶、糖原及总蛋白变化的实验结果表明：处理1~2d后钉螺的酶活高于清水对照组，处理3~4d后钉螺的酶活最强，而处理5d后钉螺样品的酶活则大大减弱。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

Characterization of senescence-associated endopeptidase iso -zymes in wheat leaves The characterization of senescence-associated endopeptidase isozymes in wheat leaves during dark-induced senescence was performed.

而采用将明胶为底物的SDS-PAGE方法只能检测到一种新内肽酶同工酶的出现，因此衰老期间表达的内肽酶同工酶大都是对SDS敏感的或多亚基的蛋白水解酶。

Selenium-dependent (glutathione peroxidases and thioredoxin reductases ) and selenium-independent (superoxide dismutases and catalase ) enzyme systems regulate cellular ROS steady state leels.

硒依赖性酶（谷胱苷肽过氧化物酶和硫氧还蛋白还原酶及非硒依赖性酶（超氧化物歧化酶和过氧化氢酶）系统共同调节使细胞内ROS处于稳态。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。