酶蛋白

DESCRIPTION】the ingredients of the product comes from abyssopelagic fishes of special pure and clean sea areas, adopting Limited Enzymic Degraded Technology, a self-owned intellectual property, that is using compound bioenzyme preparations at a low temperature to extract active ingredients through a special low-temperature extraction process and specific sheared protein to ensure the bioactivity of Collagen.

本品原料来自纯净、无污染的特定海域中的深海鱼类，采用具有自主知识产权的限制性酶切制备多肽技术（Limited Enzymic Degraded Technology），在低温下使用复合生物酶制剂，通过特殊低温萃取工艺提取有效成分，通过特异性的剪切蛋白质，保证了胶原蛋白原有的生物活性，并将原分子量为30万左右的胶原蛋白分子降解成3500左右的胶原蛋白多肽（约含30个左右的氨基酸残基），其分子长度降至30—50nm，集中度达到99.5%以上，呈白色或淡黄色，无异味，无杂物，不添加任何激素和化学物质，没有任何毒副作用。

Studies also indicated that genistein exsists not only in soybeans but also in many kinds of plants such as vegetables and fruits.As a potential anticancer agent, the actional mechanisms of genistein mainly includes as follows:First, genistein can depress the activity of protein tyrosine kinase and transduction pathways for the phosphorylation of receptors and mitosis signal. So genistein can lead to cells' proliferation depressed. Second, genistein has minimal effects of phytoestrogens. It can be combined with estrogen receptor and improve the synthesis of cellular sex hormone binding glulobin, and improve the activity of UDP-glucuronyl transferase. Through these pathways, it can inhibit the cell activity of breast cancer and prostate cancer.

作为一个很有潜力的抗肿瘤物质，三羟异黄酮的作用机制主要包括：①抑制蛋白酪氨酸激酶活性，可阻抑PTK引起的受体磷酸化和有丝分裂的信号传递，导致癌细胞增殖受抑；②弱雌激素作用：可通过与体内雌激素受体结合，并可增加细胞内性激素结合球蛋白的合成，增加UDP-葡糖醛酸转移酶的活性等途径抑制乳腺癌和前列腺癌细胞活性；③拓扑酶Ⅰ和Ⅱ抑制剂，抑制细胞活性；④上调细胞周期性负性调节因子P21WAF1/CIP1的表达，使之负性调节因子作用增强；⑤可阻止胰岛素样生长因子-Ⅰ、肝细胞生长因子和神经生长因子的作用而抑制肿瘤生长；⑥其他：抗氧化作用、抑制热休克蛋白、诱导细胞凋亡、抑制新生血管生成和抑制多种耐药相关蛋白等。

reitman method to measure ALT and AST; bilirubin oxidase method to measure total bilirubin and conjugated bilirubin; biuret method to measure serum general protein; bromocresol purple method to measure serum albumin; enzymic colorimetric method to measure serum bile acids; benzyl aldehide azo naphthol method to measure monoamine oxide.

赖氏法。测定 ALT 和 AST；胆红素氧化酶法测定总胆红素和结合胆红素；双缩脲法测血清总蛋白；溴甲酚紫法测定血清清蛋白；酶比色法测定血清胆汁酸；苄醛偶氮萘酚法测定单氨氧化酶

In our study, we also observed the different influence between TTB-USA and RFCA on blood coagulable states (by examining the change ofα-granule membrane protein、Tissue type plasminogen activatior、Plasmingen activator inhibitor-1)、 endothelium function (by examining the change of endothelin、nitric oxide) and myocardial injury (by examining the change of creatine kinase、MB isoenzy of creatine kinase and cardiac troponin T).

为观察超声消融与射频消融对血凝状态、内皮功能及心肌损伤的影响，进一步对超声消融的安全性进行评价，我们在插管前、插管后、消融后即刻、消融后30min及48小时5次抽静脉血，分别测定血小板α-颗粒糖蛋白(GMP-140)阳性表达活化血小板百分率、内皮素、一氧化氮、组织型纤溶酶原激活剂及纤溶酶原激活剂抑制物-1(PAI-1)的变化；于消融前、消融后即刻、2hr、8hr、24hr、48hr、72hr抽取静脉血，检测肌酸激酶、肌酸激酶MB同工酶及心肌肌钙蛋白T的变化。

Deamidation of the milk protein produced the spicy or ammonia-like flavor.Reduction and heat treatment had no obvious effect on the enzyme reaction and the flavor of the product.The order of different enzymes could influence the flavor effectively.In addition,the taste of the hydrolysate can not be improved by adding condiment.

结果表明，脱酰胺反应会使产品具有氨味和辛辣味；还原处理和加热处理，对脱脂乳粉和乳清浓缩蛋白的酶解速度和水解液风味都无明显影响；不同的酶解顺序对乳蛋白水解液的风味影响显著；用风味剂不能有效地掩盖酶解液的不良风味。

According to the condition of the dispase and alkaline proteinase, we combine the two enzymes so as to get the most optimization method to improve the yield of dissolvable peptide.

通过对单酶水解条件的摸索，将选择的两种酶以适当的比例混合，并且通过控制反应条件，提高蛋清蛋白的水解度和可溶性肽的得率，从而研制出一种适合蛋清白蛋白水解的复合酶。

Gene sequence was highly homologic with a gene encoding aβ- subunit of ATP synthetase and an EST originating from a cDNA in relation to water deficit stress, which both were closely associated with the protein functioning in stress resistance and ion transportation. The results suggested that No. 1 gene sequence was involved in stress resistance of apple rootstock. No. 2 gene sequence had high homology with a gene encoding a serine/ threonine kinase protein, indicating perhaps it was a new gene encoding the serine/ threonine kinase protein in apple rootstock. The gene sequences of A1 and A2 were highly homologic with a gene encoding aβsubunit of ATP synthetase.

对得到的差异片断进行回收、测序，通过BLAST和其它植物比对，发现Ⅰ号序列与一条编码ATP合酶β亚基基因和一条水分亏缺胁迫cDNA克隆基因EST的同源性非常高，该序列编码涉及到了抗性表达、离子转运基因，可能参与了植物抗性表达，说明获得的基因序列应是与苹果砧木抗性相关的基因；Ⅱ序列与一条serine/threonine kinase蛋白激酶的蛋白序列同源性比较高，核酸比对较低，可能是具有丝氨酸/苏氨酸蛋白激酶功能的新基因。A1、A2序列与一条编码ATP合酶β亚基基因的同源性较高。

The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb，用Ang Ⅱ诱导促进其增殖，采用MTT法检测细胞增殖；羟脯氨酸试剂盒检测胶原含量；ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量；流式细胞仪检测细胞周期；硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

Methods Male New Zealand white rabbits were exposed to pentavalent arsenate and trivalent arsenite 5 mg/L for 18 weeks, respectively. Levels of thioredoxin, protein and non-protein sulfhydryl and the activity of thioredoxin reductase and glutathione reductase in liver, and the levels of inorganic arsenic, monomethylarsonic acid and dimethylarsinic acid in blood, urine, and hair were measured after that.

家兔以自由饮水方式慢性暴露于无机3价砷及5价砷，18周后，测定肝脏组织中蛋白巯基、非蛋白巯基、硫氧还蛋白水平，以及硫氧还蛋白还原酶、谷胱甘肽还原酶活性，同时检测血、尿及毛发中无机砷及其代谢产物甲基砷和二甲基砷。

Nevertheless, no homologues of typical molecular chaperones have so far been found in periplasm, and the recently reported chaperone activities of periplasmic protein disulfide isomerase and peptidyl pro-lyl isomerase seem to be too weak to satisfy such assumed needs.

虽然膜间质中的蛋白二硫键异构酶和脯氨酸顺反异构酶最近被报导具有分子伴侣活性，但是这种活性的检测使用的不是天然的膜间质蛋白，而且没有捕获到复合物的形成，因此看起来仍不能够满足前面分析的对膜间质蛋白保护的需求。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。