酶蛋白

Our results suggest that COX-2 activity is involved in ethanol-induced HCV-RNA and NS5A protein expression, because acetylsalicylic acid, a COX-1/2 inhibitor, blocked this induction and downregulated COX-2 protein expression and activity.

研究结果表明，环氧合酶-2活性，涉及酒精介导的丙型肝炎病毒核酸及ns5a区蛋白表达，因为环氧合酶-1 / 2抑制剂——乙酰水杨酸，可阻断这种诱导机制，并下调环氧合酶-2蛋白的表达和活性。

One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活，ICAM-1表达升高，与被激活的PMN表面表达的LFA-1相结合，"PMN-EC"相互作用加剧，一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍；另一方面粘附的PMN过度吞噬或激活，当白细胞吞噬的颗粒不能被封闭隔离，连同细胞内的酶被释放出来，其中的PMN-elastase能够降解细胞基质中各种成分，水解多种蛋白，加重胰腺的毛细血管内皮细胞和腺泡的损伤；释放的溶酶体酶使组织蛋白水解，产生的不饱和脂肪酸引发脂质过氧化方应，破坏细胞膜的结构和功能；释放的炎性细胞因子，激发其他的免疫细胞的功能，导致进一步的组织损伤和坏死，加重SAP的病理损伤，最终导致休克、脓毒血症及多器官功能障碍等严重后果。

Several coterminous bands could be observed by PAGE of natural Eisenin Ⅰ and several coterminous peaks of Eisenin Ⅰ were also detected with MALDI-TOF-MS when the molecular weight of three main peaks are 24, 645, 25, 052 and 25, 281, separately. The amino acid composition of Eisenin Ⅰ(pI. 8) is specilized with high content of acidic amino acids and low content of alkaline amino acids; At the same time, the content of Ser and Thr are quite high and that of Lys, Met, Phe and Trp are quite low. Eisenin Ⅰ is highly homologous with serine proteases. Then, by fibrin plate assay, Eisenin Ⅰ was identified to be a plasmin and also a plasminogen activator, and the fibrinolytic activity was inhibited by PMSF (an inhibitor of serine proteases).

赤子素Ⅰ氨基酸组成特点为高的酸性氨基酸和低的碱性氨基酸含量，且Ser、Thr含量高而Lys、Met、Phe和Trp含量很低；等电聚焦电泳测得其等电点pI.8.N端序列比较结果显示赤子素Ⅰ与丝氨酸蛋白酶类高度同源，纤维蛋白平板法测得赤子素Ⅰ同时具有纤溶酶和纤溶酶激酶活性；通过PMSF对赤子素Ⅰ纤溶酶活性的抑制实验证明，赤子素Ⅰ属于丝氨酸蛋白酶类。

It was found that there were 36 distinct differences in 184 protein spots. In-gel digestion and liquid chromatography mass spectrometry C LC-MS were made to analyze the protein SSP2801, and Mascot software was applied to identify the protein. The results showed the protein was large subunit of Rubisco, and its content in yellow mutant decreased distinctly, only about 26% of the comparison. Maybe it has relation to etiolation.

切耿其中差异最为明显的SSP2801位点蛋白进行胶内酶切、LC-MS分析，并应用Mascot软件检索鉴定，结果表明该蛋白组分为RUBP羧化酶的大亚基，其在突变体中含量明显减少，仅为对照的26％，表明突变体叶色黄化与RUBP羧化酶大亚基含量减少也有关系。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

In this study, 105 TDFs were in silico mapped in the rice high-density linkage map. Nineteen TDFs were mapped to the quantitative trait loci regions for root growth under water-limited conditions in, at least, two of three rice populations derived from three crosses with the same parent of Azucena (Bala×Azucena, IR64×Azucena and IR1552×Azucena). Four of 19 genes (T37, L16, T17 and T7) were mapped based a RIL population of IR1552×Azucena by southern blot analysis. Five genes encode putative or hypothetical protein. Other 14 genes were similar with known genes in databases including expansin (OsEXP2), late embryogenesis-abundant gene , an SR-related protein essential for spliceosome assembly (SART1), autophagocytosis protein , bHLH protein, fruit-ripening protein similar to ASR, nickel-binding protein 2A, DNA-binding protein, pyruvate dehydrogenase kinase , stomatin-like protein, SR1 induced by sucrose starvation, vacuolar protein sorting protein (VSP33a), gibberellin action negative regulator and retroelement.

根据核苷酸序列将105个基因电子定位到水稻的高密度连锁图谱上，其中19个差异表达基因定位在Bala×Azucena、IR64×Azucena和IR1552×Azucena中至少两个群体共同的与根生长相关的QTLs区间，并用Southern杂交将其中的4个定位到IR1552×Azucena群体遗传连锁图谱相应的位点上。19个基因中的5个编码推断的未知功能蛋白质，其余14个编码已知功能蛋白，分别为膨胀素（Os-EXP2）、胚胎后期丰富蛋白、剪接体安装必需的SR相关蛋白（SART1）、自吞噬蛋白、碱性螺旋-环-螺旋转录因子、与ASR相似的果实成熟蛋白、镍结合蛋白、DNA结合蛋白、丙酮酸脱氢酶激酶、stomatins类蛋白、蔗糖调节蛋白SR1、液泡蛋白分类蛋白（VSP33a）、GA负调节因子、逆转座元件。

MMPs are usually secreted as latent, inactive proenzymes, which need to be activated (Fig 1 ), and/or are bound to their native inhibitors, the tissue inhibitors of metalloproteinases.8 Activation may involve proteolysis and/or oxidation of the latent enzyme.8 Proteolytic activation of the proenzymes cleaves the propeptide sequence, which masks the active site of the latent enzyme.

金属蛋白酶常以无活性的酶原方式分泌，而后者要么需要被激活要么与附近的或组织中金属蛋白酶抑制物结合。激活作用包括了具有潜在酶的蛋白水解作用或氧化作用。酶原的蛋白水解激活作用能将掩盖活性部位的潜在酶的前肽序列破坏。

The mechanism, properties of this immobilization method and response characteristics, application probables for potentiometric and amperometric enzyme-based sensors have been investigated and discussed.

以脲酶和葡萄糖氧化酶为研究对象，首次利用活蚕液状丝素蛋白的构象变化即蛋白质的变性现象，将酶固定于蚕丝素蛋白中，提出了这种固定化方法的机理、性能以及用作电位法和电流法酶传感器响应特性的研究和实际应用的可能性。

Northern blotting analysis indicated that 5 genes, nitrate transporter gene, nitrite reductase gene, ammonium transporter gene, ATP-binding cassette transporter gene, and purine permease gene, were significantly up-regulated under nitrogen starvation.

Northern blotting验证其中5个基因，包括硝酸盐转运蛋白基因、铁氧化还原蛋白亚硝酸还原酶基因、铵盐转运蛋白基因、结合ATP盒的转运蛋白基因和嘌呤透过酶基因，在缺氮诱导条件下转录水平有明显上调。

The seed purity of more than one hundred and thirty samples of six maizevarieties was assayed by using the three testing methods: storage proteins electrophoresis,isozyme isoelectric focusing in acrylamide and examination at field plots. Some importantpoints can be concluded from analyses as spearman order rank correlation on the seedpurity data as follows:First, there were deviations of the real performances to those purities obtained from out or in laboratory testing, even from the examination at field plot that is a routine way. Second, the seed globulin and isozyme electrophoresis, both with characteristics of rapid, cost-effective, devoid of environmental effect and speed, showed consistent purity; while the field-testing gave a higher degree of purity on the high side of cost than that in-lab approaches. Third, a good consistent purity was observed in the field plot and seed globulin testing, whereas inconsistency between field-testing and isozyme process. The last point is that seed globulin electrophoresis be a practical method apt to seed purity testing for maize.

并对130多份样品用三种检验方法(盐溶蛋白电泳、同功酶等电聚焦电泳和田间小区)检测的种子纯度数据作排序相关等统计分析，得到如下结果：室内外检测方法都会与种子真实状况有一定差异，田间种植鉴定与真实情况符合性并不一定最好；盐溶蛋白与同功酶等电聚焦这两种方法检测种子纯度所需时间短，成本低，不受外界环境限制，出结果快，二者的结果没有太大差异；田间种植鉴定成本较高，检测的结果普遍偏高；等电聚焦电泳与田间鉴定的一致性年度间存在差异，而盐溶蛋白凝胶电泳法与田间鉴定的一致性较高；种子盐溶蛋白凝胶电泳法较适于鉴定玉米种子纯度。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。