酶蛋白

The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明：猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同，均属不稳定类酶蛋白；保守结构域氨基酸序列具有高度的同源性，均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序；均有信号肽，属分泌型蛋白质；属于跨膜的疏水性蛋白，均有5个跨膜结构域；无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件；三维结构预测都呈"V"字形；猪和牛HMGR基因的进化关系最近。

From the mean values of our multiple experiments it seems that the τc of control F1-F0 is 5.5×10-5s, whereas the τc of the cholate treated F1-F0 is 1×10-4s.

选用参数L/L'计算其相关时间τ_c，得出对照的F_1-F_0酶蛋白的τ_e为5.5×10~(-5)秒而经胆酸处理的F_1-F_0酶蛋白的τ_e为1×10~(-4)秒。

By NCBI BLASTX server, tomato P56 amino-acid sequence was compared with other pectate and pectin lyases belonging to polysaccharide lyase family Ⅰ 28 proteins ,with higher identity of amino acid sequence compared to P56 amino-acid sequence, were obtained.

利用NCBI BLASTX服务器，搜索与番茄P56蛋白氨基酸序列相似且都属于多糖裂解酶家族Ⅰ的果胶裂解酶蛋白序列共28条，用DNAMAN软件分析其保守区，用CLUSTALX8.0软件进行序列比对和Bi-oEdit软件进行文件转换，进一步用PUAP4.0软件构建系统进化树。

Plant there exists large amount of cell wall egg white in cell wall middle, if structure egg white , zymoprotein etc., structure egg white having mainly expanding egg white , being rich in the glycine proteide and so on studying at present, brings the important effect into play in plant cell wall growth and structural adjustment.

植物细胞壁中存在大量的细胞壁蛋白，如结构蛋白、酶蛋白等，目前研究的结构蛋白主要有伸展蛋白、富含甘氨酸的蛋白质等，在植物细胞壁的生长和结构调整中发挥重要作用。

The Chitinase of Saccharomyces cerevisiae(CTS1-2) is an Endochitinase which is endogenous in Saccharomyces cerevisiae. Its recognition and slipt site lies in the β-1,4 glucosidic linkage of homopolymer. Included in the amino acid sequence from N-terminal to C-terminal of the Saccharomyces cerevisiae chitinase are signal peptide sequence, catalytic domain, binding domain and a C-terminal shorten region.

酿酒酵母几丁质酶(Chitinase，CTS1-2)是一种来自酿酒酵母的内切几丁质酶，它的酶切位点位于其同聚物内的β-1，4连接的糖苷键，酶蛋白分子结构从N端到C端依次为信号肽、几丁质催化区、几丁质结合区以及一个短的C末端区。

In this paper, turf peat, straw, wheat bran, Marc, bagasse as raw materials for bio-solid fermentation, culture feed yeast and mycoprotein, while humic acid associated biochemical and biological activity of enzymes is an ideal feed attitive of multi-enzyme protein feed, that provides a new resources of bio-protein feed for our country's livestock breeding and production of green food.

本文以草炭、秸秆、麸皮、果渣、蔗渣和酒糟等为原料的生物固体发酵，培养饲料酵母及菌体蛋白，同时伴生的生化腐植酸和生物活性酶是一种较理想的畜用多酶蛋白饲料添加剂，为我国畜牧生态养殖和绿色食品生产提供了新的生物蛋白饲料资源。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

HTERT is expressed in cancer cell lines despite promoter DNA methylation by preservation of unmethylated DNA and active chromatin around the transcription start site.

人端粒酶逆转录酶是一种核糖核蛋白酶，由端粒RNA和端粒酶蛋白组成的一种特殊逆转录酶，在激活端粒酶的过程中起限速作用，能以自身RNA为模板合成端粒序列以稳定端粒长度使细胞获得永生，也是人类端粒酶的活性中心，其表达是端粒酶活化的必须前题［2～4］。

Homology analysis demonstrated that, McChi5 protein, which contained a conserved domain of family 18 glycosyl hydrolyse, had the sequence similar to tabacco class V chitinases, several putative chitinases and chitinase-like proteins of Arabidopsis thiliania , and some chitinases from mammals, insects and bacteria.

同源性分析表明，McChi5蛋白与烟草V类几丁酶、拟南芥的推测几丁酶和类几丁酶蛋白、以及哺乳动物和细菌的一些几丁酶有序列相似性，并具有第18家族糖基水解酶的保守域。

Laccase belongs to the family of multicopper oxidases.

漆酶是一种结合多个铜离子的蛋白，是铜蓝氧化酶蛋白家族的一员。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。