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酶联免疫吸附测定

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Results TNFα-induced ICAM-1 expression in HK-2 cells was increased in both protein and mNRA levels( P .01). NFkB inhibitor N-tosyl phenylalanine chlormethyl ketonecould inhibit these effects of TNFα.

方法用 HK-2 细胞作靶细胞,用细胞酶联免疫吸附法和 Northem 杂交观察 ICAM-1 的蛋白和基因的表达,以电泳迁移率变动法测定转录因子核因子 kB和激活蛋白 1(AP-1)的活性。

Eventually, the highly specific anti-melamine polyclonal antibodies were acquired from antiserums through the purification of affinity chromatography.

采用酶联免疫吸附法测定各血清的效价,最终以亲和层析法纯化血清获得高特异性的抗体。

Methods The serum and synovial fluid of the pathologic knee joints were obtained from patients with RA,and samples from leg traumatic amputees were used as the control group.

方法用酶联免疫吸附法测定27例类风湿关节炎患者血清及病变膝关节滑液中RANTES、MCP-1水平,并与外

Methods The Biotin-Strept -Avidin enzyme linked immunosorbent assay, spectrophoto-metric assay were used to measure the plasma levels of CT-1 and sICAM-1 in 56 patients with hypertension before and after treatment of Perinopril or Benidipine and 30 normal controls.

应用生物素-链霉亲和素酶联免疫吸附法对56例高血压患者应用培哚普利和贝尼地平治疗前后及30名正常健康人,测定血浆CT-1水平和血浆sICAM-1水平。

The roots and leaves of the four reed ecotypes were determined for thelevels of abscisic acid and cytokinins using an enzyme linked immunosorbent assay throughout the whole of growing season.

采用酶联免疫吸附法,测定了整个生长季节四种芦苇生态型根和叶组织中脱落酸和细胞分裂素含量的变化。

The serum and CSF levels of IL1β, IL6, TNFα and IL1ra in the patients with different kinds of dementias and the controls were measured using ELISA.

用夹心式酶联免疫吸附法测定各组老年人血清和脑脊液中白介素1β(IL1β),白介素6(IL6),肿瘤坏死因子α和白介素1受体拮抗剂(IL1ra)的水平。

Methods: ELISA was used to determine the serum of TPA and sVCAM-1 in pre-eclamptic and normotensive pregnant women, and the correlation of pre-eclampsia and neonatal outcomes was analyzed by rank correlation.

采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)测定子痫前期患者和正常晚孕妇女外周血中TPA、sVCAM-1的浓度,并与子痫前期患者病情及新生儿体重进行相关分析。

The method of enzyme-linked immunosorbant assay was employed to determine the contents of indoleacetic acid, abscisic acid, gibberellin (GA3) and Zeatin under pot culture.

在盆栽基质培养条件下,进行了打顶和打顶+生长调节剂处理,采用酶联吸附免疫测定技术分析了烤烟根和叶中内源IAA、ABA、GA3和ZR的变化。

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