酶联免疫吸附测定

HMGB1, IL-6 levels in plasma and ultrafiltrate were measured by enzyme linked immunosorbent assay.

采用酶联免疫吸附法(enzyme linked immunosorbent assay，ELISA)测定血浆和超滤液HMGB1、IL-6水平。

Serum levels of Ang-2 and VEGF in 24 de novo, 18 complete remitted, 7 unremitted, 13 relapsed patients with AL were measured by enzyme-linked immunosorbent assay, and compared with those of normal controls.

应用酶联免疫吸附试验测定24例AL初发未治，18例完全缓解，7例未缓解，13例复发患者血清中Ang-2和VEGF的含量，并与正常对照组比较。

The patient avoided eating food rich of protein for three days and fasting for 12 hours before the blood samples were obtained at 7:00-8:00 in the morning, and had laid on bed for 15 minutes at least, 2ml of the blood samples were taken from median antebrachial vein, put into the heparinized tubles, then centrifugating by 1500/minute for 10 minutes,obtained the plasma from the blood, the plasma sample were kept in -72℃ refrigerator, ELISA method was used to determine the homcysteine level.

患者在标本采集前3天避免食用高蛋白饮食，空腹12小时以上，清晨7：00－8：00采血，采血时平卧位，休息15分钟以上，采集肘正中静脉血2ml，置于肝素抗凝管，以1500转／分离心10分钟后，分离血浆，用酶联免疫吸附法测定各管的HCY含量。

There were 12 rabbits (24 articular genus) in every group. Osteoarthritis model was established by injection of caroid in articular genu intracavitary. Rabbits with osteoarthritis were interfered by Duhuojisheng decoction. The expression of IL-1 and TNF from synovial fluid in rabbit with osteoarthritis were measured by double antibody sandwich ELISA 1, 3, 5 weeks after treatment.

将24只兔随机分为2组，即对照组和治疗组，各12只，24个膝关节，采用兔膝关节腔内注入木瓜蛋白酶造成骨关节炎的病理模型，以独活寄生汤浓缩液餵食饲养，於治疗后1、3、5 周分别抽取关节液，采用双抗体夹心酶联免疫吸附法，分别测定兔膝关节液中IL-1及TNF 表达水平。

Methods 24 rabbits were randomly divided into control and treatment groups. There were 12 rabbits (24 articular genus) in every group. Osteoarthritis model was established by injection of caroid in articular genu intracavitary. Rabbits with osteoarthritis were interfered by Duhuojisheng decoction.

将24只兔随机分为2组，即对照组和治疗组，各12只，24个膝关节，采用兔膝关节腔内注入木瓜蛋白酶造成骨关节炎的病理模型，以独活寄生汤浓缩液喂食饲养，于治疗后1、3、5 周分别抽取关节液，采用双抗体夹心酶联免疫吸附法，分别测定兔膝关节液中IL-1及TNF 表达水平。

The re were 12 rabbits (24 articular genus) in every group. Osteoarthritis model was established by injection of caroid in articular genu intracavitary. Rabbits with osteoarthritis were interfered by Duhuojisheng decoction. The expression of IL-1 and TNF from synovial fluid in rabbit with osteoarthritis were measured by double antibody sandwich ELISA 1, 3, 5 weeks after treatment.

将24只兔随机分为2组，即对照组和治疗组，各12只，24个膝关节，采用兔膝关节腔内注入木瓜蛋白酶造成骨关节炎的病理模型，以独活寄生汤浓缩液喂食饲养，于治疗后1、3、5 周分别抽取关节液，采用双抗体夹心酶联免疫吸附法，分别测定兔膝关节液中IL-1及TNF 表达水平。

There were 12 rabbits (24 articular genus) in every group. Osteoarthritis model was established by injection of caroid in articular genu intracavitary. Rabbits with osteoarthritis were interfered by Duhuojisheng decoction. The expression of IL-1 and TNF from synovial fluid in rabbit with osteoarthritis were measured by double antibody sandwich ELISA 1, 3, 5 weeks after treatment.

将24只兔随机分为2组，即对照组和治疗组，各12只，24个膝关节，采用兔膝关节腔内注入木瓜蛋白酶造成骨关节炎的病理模型，以独活寄生汤浓缩液喂食饲养，于治疗后1、3、5 周分别抽取关节液，采用双抗体夹心酶联免疫吸附法，分别测定兔膝关节液中IL-1及TNF 表达水平。

Osteoarthritis model was established by injection of caroid in articular genu intracavitary. Rabbits with osteoarthritis were interfered by Duhuojisheng decoction. The expression of IL-1 and TNF from synovial fluid in rabbit with osteoarthritis were measured by double antibody sandwich ELISA 1, 3, 5 weeks after treatment.

将24只兔随机分为2组，即对照组和治疗组，各12只，24个膝关节，采用兔膝关节腔内注入木瓜蛋白酶造成骨关节炎的病理模型，以独活寄生汤浓缩液喂食饲养，于治疗后1、3、5 周分别抽取关节液，采用双抗体夹心酶联免疫吸附法，分别测定兔膝关节液中IL-1及TNF 表达水平。

In this paper, we studied seasonal variation of endogenous IAA、 GA_3、ZR and ABA content of Leymus chinensis、 Agropyron michno、 Stipa grandis、 Cleistogenes sguarrosa、 Koeleria cristata、 Carex korshinskyi、 Allium bidentatum、 Artemisia frigida, Kochia prostrate and Potentilla acaulis in different restoring succession stages, which were determined by ELISA in the growing season in 2005. At the same time we recorded the phenological development and growing regulation of each kind of plant.

本文于2005年生长季，对处于不同恢复演替阶段的羊草、米氏冰草、大针茅、糙隐子草、草、黄囊苔草、双齿葱、冷蒿、木地肤和星毛委陵菜十种植物，运用酶联免疫吸附法，测定内源生长素、赤霉素(GA_3)、细胞分裂素和脱落酸含量的季节动态，同时记录每种植物的物候期和生长规律情况。

Endogenous hormones, including gibberellin (GA4), indole acetic acid, isopentenyl adenine and abscisic acid, were analyzed during bolting in spring radish (Raphanus sativus L.).

采用酶联免疫吸附法测定了春萝卜抽薹过程中顶端生长点处嫩叶的赤霉素(GA4)、生长素、细胞分裂素和脱落酸等含量的变化情况。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。