酶联免疫吸附测定

Methods The serum level of MMP-9 in 40 patients with IPF and 30 healthy subjects was measured by ELISA.

采用酶联免疫吸附法对40例特发性肺纤维化患者和30例健康人血清基质金属蛋白酶-9 进行测定。

Methods Serum levels of MMP-9,endostatin and VEGF were detected by ELISA method in 144 patients with lung cancer.

方法应用酶联免疫吸附法测定3种因子在144例肺癌患者血清中的表达。

Methods: Through methods of hydroxylamine and method of ELISA, We detected'the activity of SOD and quantities of PGF1a in blood plasma of thirty Sudden Hearing loss patients diagnosed by hepatopyrexia...

采用"羟胺法"和"酶联免疫吸附技术"分别测定30名突发性聋患者治疗前静脉血浆中SOD和PGF_(1α)的含量。

METHODS: EO contents of plasma and tissues in 1k1c HR were detected by ELISA. The relationships between plasma and tissues ouabain and blood pressure were analyzed.

采用酶联免疫吸附试验测定"1k1c"高血压肿瘤大鼠血清及多种组织内EO含量的改变，并对体内EO含量与大鼠血压进行相关性分析。

Methods The subjects were divided into two groups: PIH group (n= 15) and normotensive control group (n=15). Peripheral lymphocytes of the subjucts were separated by Ficoll-Hypaque density centrifugation. The lymphocytes were cultured in RPMI-1640 medium with or without pokeweed mitogen for ten days.Then the supernatant was collected and the IgG and IgM were measured by enzyme linked immunosorbent assay.

采用密度梯度离心法分离妊高征组(15例)和正常妊娠组(15例)的外周血B淋巴细胞，并在有或无美洲商陆有丝分裂原存在的情况下培养10天后，收集上清液，采用酶联免疫吸附试验测定每1×106个B淋巴细胞所分泌的IgG和IgM的量。

Methods Twenty five healthy volunteers were subjected to ETUS on the precordial region, and the parameter of ETUS was 0.8MHz, 1.2W/cm 2, the subjects were treated for 15 minutes daily for 7 days.

采用酶联免疫吸附法及发色底物法测定正常人经ETUS作用前、后，其血浆GMP 14 0、TXB2 、t PA及PAI 1含量的变化，并将经ETUS作用前、后的相应数据进行比较。

After spraying 3 kinds of growth regulators PBO,PP333 and GA_3 after full bloom,the concentration of which was 50 mg/L,the content of soluble sugar,titratable acid,soluble solid substance,vitamin C,and flesh hardness,fruit shape index,stone cell content were analyzed,and the content of IAA,GA_3,ZR and ABA in young fruit was determined by means of ELISA method to probe the dynamic change of the endogenous hormones during the fruit development.

在此基础上，于盛花期喷施浓度均为50 mg/L的生长调节剂PBO、PP333、GA_3，对各处理果实可溶性糖、可滴定酸、可溶性固形物含量及果肉硬度、果形指数和果实石细胞含量进行分析；采用酶联免疫吸附法测定了幼果生长发育过程中IAA、GA_3、ZR和ABA含量的动态变化。

Methods The serum SIL-2R level in syphilitic patients of different stages and after therapy were detected using the ELISA technique.

采用酶联免疫吸附法测定 70例梅毒患者及其治疗后SIL - 2R水平，并与 2 0名健康体检者进行比较。

Methods Monoclonal antibody turbidimetry and ELISA methods were used to detect the plasma Lp and CEA in 56 patients with large intestinal cancer and 87 healthy persons.

采用单克隆抗体浊度法和酶联免疫吸附试验法测定了 5 6例大肠癌患者和 87例健康者的血浆脂蛋白和 CEA的含量。

The uptake of 〓I-OxLDL in adipocytes was determined by gamma ray counter and normalized to protein concentration of each sample, was expressed as nanograms of 〓I-OxLDL uptake per milligram of cell protein. Reverse transcription polymerase chain reaction was used to evaluate PPARγ and CD36 mRNA expressions.

采用酶联免疫吸附法检测血浆IL-6以及脂肪细胞分泌IL-6的水平；采用gamma射线计数仪来检测脂肪细胞对〓I-OxLDL的特异性摄取情况，采用改良Lowry法进行细胞蛋白浓度测定，细胞对〓I-OxLDL的特异性摄取量以ng／mg细胞蛋白表示；采用逆转录聚合酶链式反应来检测PPAR γ和CD36 mRNA在脂肪细胞的表达情况。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。