酶联免疫吸附测定

Methods SYBR Green I real-time polymerase chain reaction was used to detect the expression of VEGF mRNA in articular cartilage from 20 OA patients and 10 controls with traumatism. VEGF protein was detected by enzyme-linked immunosorbent assay in articular cartilage.

应用SYBR Green Ⅰ实时定量聚合酶链反应检测20例OA和10例外伤患者关节软骨中VEGF mRNA表达，并用酶联免疫吸附试验测定关节软骨组织VEGF含量。

Objective To explore the clinical value of the titer of serum HP antibody in patients with gastric diseases.Methods Serous HP antibody was detected with ELISA and HP was cultured,tested with urase,and stained with Gram method on smears which was observed microscopically and compared with proteus protein-enveloped test.Results ELISA showed high sensitivity and specificity.Conclusion Serous ELISA detection of HP antibody can be used widely in clinical diagnosis and epidemical screening without endoscopy and biopsy.

目的 探索胃部疾病患者血清中的幽门螺旋菌HP抗体滴度的临床价值方法应用酶联免疫吸附试验ELISA法检测HP抗体与HP培养，尿素酶试验和涂片革兰氏染色作镜下形态检查相比较，并同时与变形杆菌蛋白包被测定法作比较结果显示该法具有敏感特异性强的特点结论该法无需常规内镜检查，活检查HP而单纯用血清学检查，可广泛应用于临床诊断和流行病学人群普查

Methods: Both IFN-γ and ADA of 80 specimens of pleural effusions (40 cases of tuberculosis pleural effusions and 40 cases of malignant pleural effusions) were analyzed by enzyme linked immunosorbet assay and ammoniacal reagent method.

用酶联免疫吸附分析法和氨试剂法检测80例胸腔积液（40例结核性积液和40例癌性积液）中IFN-γ水平和腺苷脱氨酶活性，并将IFN-γ检测结果与ADA测定结果进行对比。

Serum IgG titers against P.gingivalis antigens were measured by enzyme-linked immunosorbent assay. IgG avidity was measured by diethylamine dissociation ELISA.

血清IgG抗体滴度采用酶联免疫吸附试验测定；其亲和力通过二乙醇胺分离，ELISA测定。

Methods:The experimental group was subdivided into the bleeding and the nonbleeding groups which consisted of 64 women with IUCD.There were 60 healthy women in the control group.

采用酶联免疫吸附试验测定 64例置器妇女(试验组，试验组又分为置器出血组和无出血组)，以及60例健康妇女血清中 IL- 6水平，同时测定两组血清中 C-反应蛋白水平。

Methods The activity of plasma t-PA and PAI were detected by enzyme linked immunosorbent assay respectively in 40 cases with hypertensive disorder complicating pregnancy, 20 nonfertile women and 20 pregnant women.

采用发色底物法和酶联免疫吸附法测定40例妊娠期高血压疾病患者、20例正常非孕妇女及20例正常晚孕妇女血浆组织纤溶酶原激活物及其抑制物活性，并进行比较。

Methods: 63 patients with ACVD and 26 age matched healthy volunteers were enroued. Plasmatic levels of TF and TFPI were examined with ELISA technique.

选择脑血管病急性期患者63例和健康体检者26例；采用双夹心酶联免疫吸附抗原测定法测定患者血浆中TF和TFPI的含量，与正常对照组及组间进行比较。

All the cases were phlebotomized venously 8ml under fasting condition in the morning.Serum No and IL-6 were determined by colorimetry and radioimmunoassay method respectively.

血清NO采用硝酸还原酶法检测，血清IL-6 采用放免法检测，血浆D-二聚体和GMP-140采用酶联免疫吸附双抗体夹心法原理定量测定。

All the cases were phlebotomized venously 8ml under fasting condition in the moming.Serum No and IL-6 were determined by colorimetry and radioimmunoassay method respectively.

血清NO采用硝酸还原酶法检测，血清IL-6采用放免法检测，血浆D-二聚体和GMP-140采用酶联免疫吸附双抗体夹心法原理定量测定。

METHODS: Fasting serum levels of IL6, IL8, TNFα were measured in 30 cases of T2DM with insulin resistance and 30 cases of T2DM without insulin resistance and 26 normal control subjects by enzymelinked immunosorbent assay.

采用酶联免疫吸附法测定T2DM伴有IR患者30例和不伴有IR患者30例及正常对照26例的空腹血清白细胞介素6（IL6），白细胞介素8（IL8）及肿瘤坏死因子的浓度，并同时测定空腹血糖、糖化血红蛋白（GHbA1c）及空腹胰岛素水平，分析其与IL6，IL8，TNFα的相关性。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。