酶联免疫吸附测定

In addition, Immune nephelometry assay was imployed to analysis the level of rats' plasmic Fg, in the mean time, Platelet aggregation meter was used in observing the ratio of rats' platelet aggregation at 3 min. and 10 min, respectively.

实验采用酶联免疫吸附法测定各组大鼠血浆中TXB2、6-Ke-PGF1α、PGE2的水平；另外，采用免疫比浊法测定两组大鼠血浆中纤维蛋白原的含量，并同时观察各组大鼠3min，10min内的血小板聚集率。

61Healthy persons were selected as healthy group. The serum concentration of TGF-β1 was measured by ELISA method; nitre oxide was measured by nitrit reductase method, plasma Ang II was measured by radioimmunoassay.

对高血压病人和61例健康对照者应用酶联免疫吸附法测定血清TGF-β_1浓度，硝酸还原酶法测定NO的浓度，放射免疫分析法测定Ang Ⅱ水平。

Methods The serum sICAM-1 was measured by ELISA in 20 patients with pollinosis and in 2 0 healthy persons as normal control group.The serum tIgE was analyzed with the P harmacia Uni-CAP System for two groups but Artemisia pollen-specific IgE was a ssayed only for patents group.

对20例花粉症患者及20名健康人采用酶联免疫吸附实验测定血清sICAM-1，用荧光免疫法测定血清tIgE和蒿草花粉特异性IgE(W6)。

Methods Among 86 patients in observation group with acute coronary syndromes,there were 47 cases with unstable angina pectoris and 39 cases with acute myocardial infarction,and 40 cases of health people in contrast group.The serum level of sICAM1 and sVCAM1 were detected by ELISA,and CRP was detected by immune turbidimetry in all cases.

选取ACS患者86例为观察组，将观察组分为不稳定型心绞痛和急性心肌梗死两组，UAP组47例，AMI组39例，另选健康成人40例为正常对照组，采用酶联免疫吸附法测定各组血中sICAM1和sVCAM1水平，采用免疫浊度法测定各组血中CRP浓度，并对其结果进行比较。

Serum alt,ast,and bile acid were observed by enzyme assay and the ratio of ast/alt was worked out. serum bilirubin was measured by peariman and lee method. serum albumin was tested by bromphenol assay and was simultaneously measured to hyaluronic acid, type ⅲ procollagen, laminin and type ⅳ collagen by ria. meanwhile, prothrombin time and platelet count were measured and so on. results: the level of serum aca was raised increasingly in different cirrhotic cases with different classifications of child-pugh, according to severity of the condition.

应用酶联免疫吸附法分别对不同分级的肝硬化患者血清中抗心磷脂抗体的含量进行了测定；用酶法测定了alt、ast与胆汁酸并计算ast/alt值；用peariman和lee改良法测定血清胆红素；用溴甲酚氯比色法测定血清白蛋白；用放射免疫分析法测定透明质酸、ⅲ型前胶原氮端肽、层粘连蛋白与ⅳ型胶原；并测定了凝血酶原时间与血小板计数等相关指标。

Change in the concentration of endogenous IAA was measured with enzyme-linked immunosorbent assay, and the distribution of IAA-binding protein in the protoplasts in the cambial region was tested with an immuno-fluorescence technique, being improved by the authors, during the cambial active period in Broussonetia papyrifera L.

构树（Broussonetia papyriferaVent。）形成层活动周期中用酶联免疫吸附法测定了形成层区域的内源IAA浓度的变化，并用自行发展的免疫荧光检测方法测定了由此处生活细胞制得的原生质体中IAA结合蛋白的分布。

Cases healthy subjects served as control. In addition, density of glomerular matrix membrane and quantity of cell in glomeruli were measured with CMIAS image analysis system by computer. Global sclerosis, cellular crescents, fiber or fibrocellular crescents were measured with Memphis scope. Tubulointerstitial lesions were examined with semi-quantitative grades (including O-III grades).Results Compared to normal control, there was a significant increase of urinary TGF-betaK IL-6 and Col-lV levels in patients with IgAN (p. 01). There were also significantly positive correlation between the levels of urinary TGF-betaK Col-IV and density of glomerular matrix membrane ., interstitial fibrosisp.

采用酶联免疫吸附法测定尿TGF-β1(Transforming growth factor-β1)、尿IL-6(interleukin-6)和尿Col-Ⅳ(type Ⅳ Collagen)；采用免疫组织化学法检测肾组织TGF-β1、Col-Ⅳ表达；应用CMIAS多功能真彩色病理图像分析系统对肾小球基质基底膜面密度和细胞个数进行半定量测定；对球性硬化、节段硬化、细胞新月体及纤维或纤维细胞新月体所占肾小球百分数和肾小球IgA沉积免疫荧光强弱的判断采用Memphis法进行评分；肾小管间质病变程度采用光镜下半定量分级法(包括0-Ⅲ级)。

Methods 45 patients with nonobstructive azoospermia, 40 patients with obstructive azoospcrmia and 40 healthy subjects were involved in this study. Serum FSH, LH and T levels were measured by chemiluminescence immunoassay, and serum INHB was measured by sandwich enzyme-linked immunosorbent assay.

采用化学发光免疫分析法测定45例非梗阻性无精子症、40例梗阻性无精子症及40名正常生育男性血清FSH、LH及T的浓度，以双抗体夹心酶联免疫吸附试验测定血清INHB水平。

The rats of model group,acupointⅠgroup, acupointⅡgroup were treated with passive smoking.The rats of acupointⅠgroup,acupointⅡgroup were treated with placing Chinese medicine at the point of feishu,pishu for 5 hours or 10 hours after depilation.

治疗结束后观察支气管组织病理特征及细胞浸润情况，并对其进行气道炎症病理评分；酶联免疫吸附法测定被动吸烟大鼠肺泡灌洗液中α_1-抗胰蛋白酶、TNF-α、IL-8含量；黄嘌呤氧化酶法测定被动吸烟大鼠肺泡灌洗液SOD的活性、TBA法测定MDA的含量。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。