酶联免疫吸附测定

Seroepidemiologic Studies ;; Measles ;; Rubella ;; Exanthema ;; Antibodies, Viral ;; Fever ;; Disease ;; Immunoglobulin M ;; Enzyme-Linked Immunosorbent Assay

血清流行病学研究；；麻疹；；风疹；；疹；；抗体，病毒；；发热；；疾病；；免疫球蛋白M ；；酶联免疫吸附测定

Methods level of telomerase activation and PCNA expression were detected in 80 cases of breast cancer,36 cases of side-cancer tissues and 10 cases of lacteal gland benign lesion using TRAP-PCR-ELISA and S-P immunohistochemistry technique.

采用端粒重复序列扩增聚合酶链反应-酶联免疫吸附测定法(TRAP-PCR-ELISA)和免疫组织化学链霉素生物蛋白过氧化物酶法对80例乳腺癌组织、36例乳腺癌相应癌旁组织和10例乳腺良性病变的端粒酶活性及增殖细胞核抗原进行检测。

Methods The level of telomerase activation and PCNA expression were detected in 80 cases of breast cancer,36 cases of side-cancer tissues and 10 cases of lacteal gland benign lesion using TRAP-PCR-ELISA and S-P immunohistochemistry technique.

采用端粒重复序列扩增聚合酶链反应-酶联免疫吸附测定法(TRAP-PCR-ELISA)和免疫组织化学链霉素生物蛋白过氧化物酶法对80例乳腺癌组织、36例乳腺癌相应癌旁组织和10例乳腺良性病变的端粒酶活性及增殖细胞核抗原进行检测。

Methods The level of telomerase activation and PCN A expression were detected in 80 cases of breast cancer,36 cases of side-cancer tissues and 10 cases of lacteal gland benign lesion using TRAP-PCR-ELISA and S-P immunohistochemistry technique.

采用端粒重复序列扩增聚合酶链反应-酶联免疫吸附测定法(TRAP-PCR-ELISA)和免疫组织化学链霉素生物蛋白过氧化物酶法对80例乳腺癌组织、36例乳腺癌相应癌旁组织和10例乳腺良性病变的端粒酶活性及增殖细胞核抗原进行检测。

Methods Twenty-two cases of nasal polyp and 10 cases of normal inferior turbinate were studied with immunohistochemical SABC method and ELISA method.

应用免疫组化和酶联免疫吸附测定法，对22例鼻息肉和10例对照组下鼻甲组织内IL-8 的分布及含量进行测定。

In present study, zooblooting, ELISA and whole-mount immunocytochemistry methods were used to identify the FXPRLamide family neuropeptides from the Japanese oak silkworm, Antheraea yamamai.

利用Zooblotting、酶联免疫吸附测定和免疫组织化学等方法，对天蚕的DH、PBAN等FXPRLamide家族神经肽进行鉴定。

The morphological and cytopathological characters of the viruses were observed by electron microscopy The types of virus were identified with I-ELISA, DAS-ELISA, TAS-ELISA, RT-PCR and IC-PCR. The CP genes of the main viruses identified in this research were amplified by PCR, cloned into pGEM-T and sequenced. The pumpkin germplasm from Heilongjiang and Yunnan provinces was screened for resistance to the main viruses using friction and roots immersed inoculation.

应用电子显微镜负染法和超薄切片法观察病原粒体形态及细胞病理学特征；利用酶联免疫吸附测定法中的双抗体夹心ELISA、间接ELISA、三抗体夹心ELISA以及反转录聚合酶链式反应、免疫捕捉PCR方法鉴定了采集样品中的病毒种类；对被确定为南瓜主要病毒病原的外壳蛋白基因进行了PCR扩增，克隆到pGEM-T载体并进行测序；应用摩擦接种法和浸根接种法对云南、黑龙江省部分南瓜品系、品种进行了抗性筛选。

This study paid more attention to the techniques of detecting Soybean mosaic virus in dry soybean seed and Bean common mosaic virus in dry bean seed by reverse transcription-polymerase chain reaction, combining with biological test and enzyme-linked immosorbent assay. The goal of the research is to establish a molecular detection procedure of SMV and BCMV from dry seed. The main results are following:⑴The effective techniques were made to extract total RNA of SMV from dry soybean seed and BCMV from dry bean seed.

本研究以大豆和菜豆种子中主要种传病毒大豆花叶病毒、菜豆普通花叶病毒作为检测对象，在生物学测定和酶联免疫吸附测定的基础上，研究干种子中两种病毒的逆转录-聚合酶链式反应检测方法，以建立一套快速、灵敏、专化性强的直接以干种子作为检测对象的分子检测技术体系，主要结果如下：⑴建立了从大豆和菜豆干种子中提取SMV、BCMV总RNA的有效方法。

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清，将纯化的抗磷酸酪氨酸抗体作为第一抗体，羊抗兔Ig酶结合物作为第二抗体，建立双抗体夹心酶联免疫吸附测定法。

Crohn's disease was induced by trinitrobenzene sulfonic acid in the model group. The mice were sacrificed 5 days afterwards and LPMC were isolated and cultured. The expression of CCR2 and CCR5 on LPMC were determined by flow cytometry. The secretion of MIP-1α in the supernatant was determined by enzyme-linked immunosorbent assay, and the expression of MIP-1α mRNA was determined by reverse transcriptase polymerase chain reaction.

模型组小鼠以三硝基苯磺酸灌肠造模。5d后处死，提取和培养LPMC，采用流式细胞仪测定小鼠LPMC中趋化因子受体CCR2、CCR5的表达；采用酶联免疫吸附测定检测培养上清液中MIP-1α的分泌；采用逆转录聚合酶链反应检测MIP-1α mRNA的表达。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。