酶的

The results indecated that 1-MCP treatment delayed the decline of fruit titratable acidity content, increased vitamin C accumulation, not affect the change of TSS. The respiration rate and climacteric peak of fruit was inhibited by 1-MCP treatment. The fruits treated with 1-MCP had higher firmness, lower pectase activity.

结果表明，1-MCP处理能够延缓果实酸度的降低，增加维生素C的含量，对可溶性固形物的变化没有影响；降低呼吸强度，抑制呼吸跃变的发生；抑制果胶酶的活性，延缓果实硬度的下降；处理前期对过氧化物酶的活性有抑制作用。

Replicas of DNA are made when the double helix unwinds as a result of helicase enzyme action and the separated strands serve as templates along which complementary nucleotides are assembled through the action of the enzyme DNA polymerase. The result of the two new molecules of DNA each containing one strand of the original molecule, and the process is termed semiconservative replication.

DNA 的复制在解旋酶解开 DNA 链时开始，解开的链作为模板 DNA 聚合酶的作用下结合相对应的核苷酸，最终形成了两条新的 DNA 的链，每一条的链含有一条链原来的 DNA 单链，因此又叫做半保留复制。

Studies also indicated that genistein exsists not only in soybeans but also in many kinds of plants such as vegetables and fruits.As a potential anticancer agent, the actional mechanisms of genistein mainly includes as follows:First, genistein can depress the activity of protein tyrosine kinase and transduction pathways for the phosphorylation of receptors and mitosis signal. So genistein can lead to cells' proliferation depressed. Second, genistein has minimal effects of phytoestrogens. It can be combined with estrogen receptor and improve the synthesis of cellular sex hormone binding glulobin, and improve the activity of UDP-glucuronyl transferase. Through these pathways, it can inhibit the cell activity of breast cancer and prostate cancer.

作为一个很有潜力的抗肿瘤物质，三羟异黄酮的作用机制主要包括：①抑制蛋白酪氨酸激酶活性，可阻抑PTK引起的受体磷酸化和有丝分裂的信号传递，导致癌细胞增殖受抑；②弱雌激素作用：可通过与体内雌激素受体结合，并可增加细胞内性激素结合球蛋白的合成，增加UDP-葡糖醛酸转移酶的活性等途径抑制乳腺癌和前列腺癌细胞活性；③拓扑酶Ⅰ和Ⅱ抑制剂，抑制细胞活性；④上调细胞周期性负性调节因子P21WAF1/CIP1的表达，使之负性调节因子作用增强；⑤可阻止胰岛素样生长因子-Ⅰ、肝细胞生长因子和神经生长因子的作用而抑制肿瘤生长；⑥其他：抗氧化作用、抑制热休克蛋白、诱导细胞凋亡、抑制新生血管生成和抑制多种耐药相关蛋白等。

In the course of establishing standard operating procedure, The temperature, time , the feasibility without decorticating and efficiency were investigated when alliinase was deactivated used a dry heating apparatus in tunnel type, but the efficiency can be achieved less than 70%, need to be improved; The reformative cribriform plate has been proved to be good in practice result in pulping enzyme-deactivated garlic without dehulling; The extraction efficiency achieved more than 90% when volumic ratio of 20% alcohol and water is 3 I 1 and 2:1 was adopted to extract alliin two times respectively; During exact filtration, the ratio of diatomite in precoating and filtration was 3% and 1% respectively, the losses of alliin adsorbed by diatomite in extraction solution were not remarkable. In the stage of alliin isolation by ion exchange resin, three simple and sensitive detected measures were successfully adopted to control the whole process; the efficiency of alliin isolation was similar to laboratory results; The alliin yield was over 50% in acidic fractions and the purity quotient of alliin achieved above 50% after drying; Furthermore, the phenomenon was found that losses of alliin were seriously affected by the temperature and time periods in concentration.

在制定中试生产标准操作规程中，考察了隧道式干热烘干机杀酶的温度、时间和效率，干热灭酶效率只能达到70%，待改进；改良的筛板使带皮打浆效果良好，证明非脱皮熟蒜打浆的可行性，减少了工序，降低了成本；提取过程中，20%酒精分别1∶3和1∶2提取2次，效率达90%以上；硅藻土板框过滤时，探索了精滤过程中提取液中预涂和过滤时硅藻土的比例分别为3%和1%，并考察硅藻土对蒜氨酸的吸附损失，滤液澄明度良好，蒜氨酸损失很少；在树脂吸附分离阶段，采用三种简便灵敏检测手段控制生产过程，分离蒜氨酸的效果与实验室一致，酸性流分中蒜氨酸收率可达50%以上，干燥后蒜氨酸纯度达50%以上；试验发现浓缩蒜氨酸洗脱液时温度和浓缩时间对蒜氨酸损失的具有很大影响。

Although the biological significance of DNA methylation has been recognized to some extent, the mechanisms of DNA methylation regulation and of the establishment of tissue-specific DNA methylation pattern in somatic cells are still unclear, since the knowledge of the enzymes responsible for de novo DNA methylation and demethylation of DNA is very limited and up to now only two functional DNA methyltransferases in mammals have been cloned.

目前，虽然对DNA甲基化的作用已有一定认识，但DNA甲基化的调节以及发育过程中组织特异的DNA甲基化谱的建立机制还不清楚。问题主要在于对催化重新甲基化和去甲基化反应的酶的了解很少。至今仅克隆出两种有功能的哺乳动物DNA甲基转移酶。

The Schiff base transitional metal complexes of various structures and their metallomicelles as peroxidase models for the phenol and phenolic derivant oxidation have been investigated. Some results are obtained from the studies:(1) the Schiff base transitional metal complexes have high catalytic activity for the phenol and phenolic derivant oxidation, and the structure of the complexes has a great effect on its catalytic activity;(2)a novel kinetic mathematical model for the complexes and their metallomicelles catalyzing the phenolic derivant oxidation has been established;(3) the feasibility and mechanism for the phenolic derivant oxidation catalyzed by the metallomicelle(as the mimetic model of peroxidase)have been discussed.

研究了冠醚Schiff碱金属配合物作为过氧化物酶的模拟模型对酚类物质氧化反应的催化作用，取得如下结果：(1)几种冠醚Schiff碱金属配合物对催化酚类物质氧化具有良好的催化活性，其中配体结构对其催化活性有较大的影响；(2)建立了金属配合物与表面活性剂形成的金属胶束催化酚类物质氧化反应的动力学数学模型；(3)探讨了金属胶束模拟过氧化物酶催化酚类氧化反应的可行性及其机理。

The result shows that the chitosan can increase the the amount of effective tiller, then the yield of both of cultivars of Zizania caduciflora is enhanced markedly, and the concentration of 45mg/L of the chitosan has the best effect. The activity of POD in leaves and the content of sucrose in product can be increased after using the chitosan, and the change of the chlorophyl content could be abated and the effect has a long duration, and the furthersome effect of chitosan on the activities of the CAT and the APX lasts in the prophase and the midphase of growth.

结果显示壳聚糖的使用对单季茭和双季茭的有效分蘖数都有增加作用，从而明显提高茭白产量，并且均以45mg/L的浓度效果最好，其使用能显著提高两种类型茭白生长期叶片中POD酶的活性以及茭体中的蔗糖含量；壳聚糖对茭白叶绿素有缓和变化波动的作用且持效久，对茭白CAT、APX酶活性的促进作用只在处理前中期有效；而对株高和A.A。

The relation between accessibility of amino acids and the property of enzyme was discussed, and the relation between accessibility of amino acids and the isoelectronic point of enzyme was studied.

酶总是在一个特定的pH范围内发挥功能。酸碱性质是它的一个重要的理化性质，等电点是它的一个重要的参数，研究它有利于更好的认识酶的功能。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7％),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3％ and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌，其中革兰阴性杆菌225株（78.4％），革兰阳性球菌41株（14.3%），念珠菌21株（7.3%），检出菌来自呼吸道标本占63.4%，其他标本各占5%左右；细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%；耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%；大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%，铜绿假单胞菌对亚胺培南的耐药率为40.2%；白色念珠菌对氟康唑的耐药率为81.3％，对两性霉素的耐药率为3.2%。

However, with the development and the application of new techniques, the reliability of the methods used in the research of the transcription site of RNA polymerase I was doubted; and also, some indirect results indicated that the transcription of RNA polymerase Ⅲ might occur in the nucleoli.

但随着新的研究技术的发展和应用，人们对RNA聚合酶Ⅰ转录位点的早期的研究方法的可靠性提出质疑；同时，根据一系列间接的实验推论RIgA聚合酶Ⅲ的转录可能发生在核仁中。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。