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The effects of FPL andits three ingredients on growth of ovary,on micro-and ultra-structure andAlkaline phosphatase activity of endometrium,on structure andkeratinization rate of exfoliated cells in vaginal epithelium in mice,onmicrocirculation in rat and on uterine kinesis in rabbit were respectivelyobserved by the techniques of light and electron microscopes,histochemistry,microcirculation and organ cinetography.The results have confirmed themechanism of FPL for the sterility caused by ovary standstill and persistentcorpus luteum to be as follows:(1) a weak estrogen-like role which canprompt development of ovary,proliferation and secretion of endometrium andvaginal epithelium and cornification of exfoliated cells in vaginal epithelium;(2)enhancing AKP's activity of endometrium;(3)improving microcirculation;(4) exciting uterus.

分别采用光学显微镜和电子显微镜技术、组织化学技术、微循环技术和器官运动描记等现代医学实验技术,观察了促孕液对小白鼠的卵巢发育、子宫内膜的显微和超微结构和碱性磷酸酶活性、阴道上皮结构和脱落细胞的角化率、对大白鼠的微循环和对家兔的子宫运动的影响,结果证实了促孕液治疗卵巢静止、持久黄体的机理:(1)微弱的雌激素样作用,能刺激卵泡发育,促进子宫内膜和阴道上皮增生、分泌和阴道上皮脱落细胞角化;(2)提高子宫内膜碱性磷酸酶的活性;(3)改善微循环;(4)促进子宫运动。

The protein and phospholipid constituent in membrane of neurocyte in brain of chicken with encephalomalacia was separated and studied with the method of SDS-PAGE electrophoresis and the method of high performance-thin layer chromatography respectively The fluidity of neurocyte membrane of chicken with encephalomalacia was determined for the first time The content of Ca and trace elements were determined in chicken encephalomalacia systematically and the status of Fe ion in cerebellum was observed. The activity of enzymes that were relative to metabolism of energy and neuro-medium in brain were checked systematically with the method of enzyme-histochemistry. Pathological examination was carried out by microscopic and ultramicroscopic observation. The apoptosis of neurocyte in brain was checked.

在进行临床观察、定期剖杀并检测体内V〓含量、体重和脑重的基础上,重点研究了与神经损伤有关的以下几个方面的内容:系统地检测了体内自由基代谢和抗氧化系统功能的变化;首次采用SDS-PAGE电泳法分离并检测了雏鸡脑软化症神经细胞膜蛋白组分,并以高效薄层层析法检测神经细胞膜磷脂组分;测定了脑软化症雏鸡神经细胞膜的膜流动性;首次系统检测了脑软化症雏鸡体内钙及微量元素含量,并进行了小脑组织总铁离子状态观察;采用酶组织化学方法系统地观察了脑组织中与脑能量代谢和神经递质有关的酶的活性;对相关组织进行了病理组织学和超微结构观察;并对脑组织中神经细胞的凋亡进行了检测。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer, In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed.

加入诱导剂RU486后,可以诱导表达荧光素酶,并在一定范围内两者呈正比,最高可以实现荧光素酶的40余倍的表达,而没有RU486时,几乎没有报告基因的表达,表明RU486诱导调控载体构建成功,可实现对目的基因的表达时间和表达水平的精确调控,为进一步的基因调控研究和基因治疗提供了良好的工具。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer. In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed. There was a positive correlation between the luciferase activation and RU486 concentration in a definite range. The results showed that the RU486-inducible regulatory vector was successfully constructed, which can be used to regulate the expression level of genes of interest in appropriate time by the inducer RU486. This inducible expression vector provides a platform for the research of gene regulation and gene therapy.

加入诱导剂RU486后,可以诱导表达荧光素酶,并在一定范围内两者呈正比,最高可以实现荧光素酶的40余倍的表达,而没有RU486时,几乎没有报告基因的表达,表明RU486诱导调控载体构建成功,可实现对目的基因的表达时间和表达水平的精确调控,为进一步的基因调控研究和和基因治疗提供了良好的工具。

A number of lines evidence indicate that calcium treatments maintain the structure and function of the cell walls, increase the fruit firmness, decrease the activity of the cell wall degration enzymes and ethylene production, maintain the integrity and stability of the membrane, activate some oxidases, induce phytolexin and regulate cell function as a signal mobecular.

钙的防腐机制在于对果蔬采后腐烂的控制,保持细胞壁的结构与功能,增强果实硬度,降低细胞壁降解酶的活性,抑制呼吸,降低乙烯生成量,保持细胞膜的完整性,激活体内的某些氧化酶,诱导合成植保素,作为信号分子,调节细胞功能。

The amount ofethylene release by shoot apex and root showed no difference between iron-deficient treatment and control plants above before the appearance ofrelatively high reductase activity,which indicated that ethylene may not playa direct role in regulation of root ferric reductase activity,and the inhibitionof which by〓and〓may be caused by other reasons.

缺铁处理植株茎尖和根系乙烯释放量的测定结果显示,三种植物中,缺铁与对照植株的茎尖及根系所释放的乙烯可能不直接参与缺铁根系还原酶的调控,〓和〓对缺铁根系〓还原酶活性的抑制可能是其它原因所致。

Although the study results show that extraction of the enzyme can only remove the clothes some, but not all of the bloodstains, but the researchers are still trying to use a mixture of enzymes, or try a different snake saliva to achieve a better cleaning effect.

尽管这项研究结果表明,酶的提取只能删除一些衣服,但不是所有的血迹,但研究人员仍试图使用一种酶的混合物,或尝试不同的蛇唾液获得更好的清洗效应。

Meanwhile, five proteins that were also induced, were proved to be the enzymes involved in the process for glycolysis or gluconeogenesis.

在对表达差异显著的15个点进行了鉴定后,确定了其中5个属于糖酵解过程的关键酶,说明底物不仅诱导了直接作用于底物的氧化酶的表达而且导致了代谢的整体迁移。

This research utilized the method of organic synthesis, researching different amounts and positions of hydroxy-substituted benzoic acid and serotonin into amide reaction, so as to produce a series of polyhydroxy substituted N-benzoylserotonin derivatives (compound 1-10). Accordingly, this study conducts the following four tests. I. The activity of antioxidant and the experiment of ability to scavenge DPPH free radical, II. The ability of overall antioxidant—the activity of TEAC, III. The activity test of inhibit Tyrosinase, and IV. The study of absorbing UV / VIS Spectrophotometers. This study use trolox, arbutin, and octyl salicylate as the control groups, and to test the ability of anti-oxidation, the activity of inhibit Tyrosinase, and the ability of absorbing UV. This research ultimately intends to investigate the applied value of those compounds into cosmetics.

本研究主要利用有机合成的方法,将不同数量及不同位置的羟基取代苯甲酸化合物与色洛冬宁进行醯胺化反应后,得到一系列的多羟基取代之N-色洛冬宁苯甲醯胺衍生物(化合物1 ~ 10),进行四种不同活性测试方法:一、清除DPPH自由基能力抗氧化活性测试;二、总抗氧化能力活性测试;三、抑制酪胺酸酶活性美白测试;四、紫外线/可见光光谱仪吸收测试,并以水溶性维生素E、熊果素、水杨酸辛酯当作对照组,探讨其抗氧化能力、抑制酪胺酸酶的活性能力与吸收紫外线能力,再进一步探讨其应用於化妆品上的价值。

Furthermore, strategies combining exogenous BNP with an inhibitor of the BNP-degrading enzyme neutral endopeptidase could contribute to maximising the actions of BNP and reduce the amount of exogenous BNP needed.

而且,BNP降解酶中性内肽酶的抑制剂和外源性BNP的合用将会最大的提高BNP的作用,减少外源性BNP的用量。

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