酶的

In thes subject, I have analyzed the developing conditions of esculen packing inland andoverseas, according to ourselves level, adopting cheap bean dregs as material, usingbiological zymolysis technology, biological protease and lipase as catalyzer andthrough the catalysis of enzyme tO make soybean fibre of bean dregs and then form newesculent packing.

本课题通过分析国内外可食性包装的发展状况，结合我国的实际情况，采用价格低廉的豆渣为原料，运用生物发酵技术，以生物蛋白酶、脂肪酶为催化剂，经酶的生物催化作用提取豆渣中的膳食纤维，制成新型可食性包装。

To study the molecular structure and function of the PyNPase, and to construct a system for preparation of the enzyme for practical synthesis of nucleoside analogs, molecular cloning of the encoding the PyNPase from E. aerogenes strain EAM-Z〓 have now been completed.

我们通过以该酶的N端氨基酸序列设计上游引物，以不同来源PyNPase的C末端同源区氨基酸序列设计下游引物，采用PCR技术，扩增出该酶基因的编码区（约1206bp），并完成了其重组质粒的构建和核苷酸序列的测定，目前正在进行PyNPase表达质粒的构建。

"Leukemic cells that resist asparaginase and survive in this protective niche of the bone marrow might be the reason that leukemia recurs in some children who have been treated with this drug," said Dario Campana, MD, PhD, Oncology and Pathology.

Dario Campana，肿瘤学和病理学的医学博士和理学博士，说：&白血病细胞抵抗天冬酰胺酶的效果，从而在这种保护性的小生境中存活下来可能是一些接受了天冬酰胺酶治疗的儿童白血病复发的原因。&

Esculetin had the highest affinity toward the binding site of XO, and this was due to hydrogen bonding interactions. The hydroxyl group at C-6 formed hydrogen bonds with the carboxyl group Glu802 that played critical role on XO binding.

在8种香豆素衍生物中发现esculetin与黄嘌呤氧化酶的亲和力最高，因为在esculetin的结构上，C-6的OH基团可与麸胺酸802的COOH基团形成氢键，此键结对於抑制黄嘌伶氧化酶活性是很重要的，因而增加了两者结合的稳定性。

The digestion process includes the following steps: adding 0.15 % concentration collagenase II solution compounded with DMEM into blood vessel smooth muscle tissue block and acting in water bath for 1.5 hr until forming floccular tissue; cleaning with 0.02 % EDTA solution, adding 0.125 % trypsase solution and water bath vibrating to digest at 37 deg.c to obtain dispersed smooth muscle cells; terminating enzyme action with 10 % new born ox serum culture liquid; regulating cell concentration with 20 % new born ox serum culture liquid to 50,000-500,000/L, inoculation, and culturing in culture tank with 5 % CO2 and first 37 deg.c to cell fusion of 70-80 %; and subculturing conventionally.

向血管平滑肌组织块中，加入用DMEM配制的0.15％胶原酶II溶液，水浴箱中作用1.5h，至组织呈絮状，用0.02％的EDTA清洗，然后，加入0.125％胰蛋白酶溶液，于37℃水浴振荡消化，即可得到分散的平滑肌细胞，用10％新生牛血清的培养液终止酶的作用。加入含20％新生牛血清的培养液，调节细胞密度为5×104～5×105个/L，接种，放入5％CO2、37℃培养箱中培养。当细胞达到70％～80％融合时即可按常规方法传代。

The enzyme amplified lanthanide luminescent spectrometry of horseradish peroxidase was developed and applied to the analyse of HRP and tuberculosis. The deconvolution curve method was used for synchronous fluorimetric determination of two component systems of highly overlapped spectra, such as phenol and p-dihydroxybenzene, phenol and o-dihydroxybenzene, and tyrosine and tryptophan. A novel mathematical method, combining Gorry's deconvolution method, was presented to remedy data loosing of SavitzkyGolay's deconvolution method and applied to dealing with fluorescent signal.

建立了辣根过氧化物酶的酶联放大镧系发光法，并应用于结核抗体的测定；成功地应用褶合曲线分析法并进行了改进，结合同步荧光光谱法用于苯酚/对苯二酚、苯酚/邻苯二酚和酪氨酸/色氨酸等光谱重叠较严重的双组份体系的同时测定；结合Gorry提出的卷积法，提出一种解决Savitzky-Golay卷积法中两端信息丢失问题的快捷方法。

Consequently, the enzymatic hydrolyzable P may contribute to the internal loading of the eutrophic lake, and stratification of kinetics and compositions of phosphatase in shallow lake is theoretically noticeable.

因此，富营养湖泊沉积物中磷的&内负荷&应有可酶解有机磷的含义，浅水湖泊中磷酸酶的活性组成和动力学参数的分层现象当为湖沼学研究中值得深入探讨的新理论问题。

Mutant libraries created by saturation mutagenesis of each single amino acid site D168, A225, K434 and E435 were screened to further improve the capability of cytochrome P450 BM-3(A74G/F87V/L188Q) mutant from Bacillus Megaterium which can hydroxylate indole into indigo. Results showed that D168, K434 and E435 are located at the functional domain of P450 BM-3 protein while A225 is sited at the unfunctional domain.

为了进一步获得具有更高活力的细胞色素P450 BM-3(F87V/A74G/L188Q)突变酶，利用饱和突变技术对该突变酶的4个氨基酸位点D168、A225、K434、E435分别进行单一的随机突变，通过对其羟基化吲哚生成靛蓝的催化性能表征，发现P450 BM-3氨基酸残基的168、434和435位均位于蛋白功能区域，而225位则位于非功能区域。

During our strategy, two linear amplification approaches are employed, ligase detection reaction and an isothermal amplification (cooperation by DNA polymerase and nicking enzyme).

连接产物由于在DNA聚合酶和限制性内切酶的共同作用下，发生等温扩增反应，扩增出大量的血红素的适配子，适配子与血红素结合，形成的复合物催化鲁米诺和双氧水的化学反应。

The activty of SOD in vesicle solution is then determined. But still no apparent superactivty is found due to the following reasons: 1. The lowered dielectric constant reduces not only the self-oxidation rate of pyrogallic, but also the activity of SOD; 2. There are no efficient enough interaction between vesicles and SOD.

在上述体系中进行的SOD的活性研究中，没有发现表观超活性，估计是两方面的原因：一是囊泡周围低的介电常数同样也降低了SOD酶的活性，二是由于表面活性剂浓度较小，仅为0.5％左右，使得囊泡体积分数较小，不能与SOD酶发生充分有效的结合。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。