酶的

Methods The system employs the 5' 3' nuclease activity of Taq DNA polymerase to digest a salmonella specific internal fluorogenic probe which is labeled with two fluorescent dyes and hybridizes to the target DNA during PCR.

利用TaqDNA聚合酶的 5' 3'核酸外切酶活性降解特异性杂交于模板上的荧光标记探针，导致荧光强度的改变，通过检测荧光强度分析PCR扩增产物。

Three donor wheat all show good character and had little genotypical difference in the aspect of inducement、differentiation、regeneration etc. They could all nice genotypical cultivars for wheat transformation. 3160 embryos have been used to transformation (960 embryos belongs to Atlas66, 1000 embryos belongs to EM12, 1200 embryos belongs to Scout66 respectively), At last, we got twenty-six of which were positive cultivars through PCR. The preliminary whole transformation frequency was 0.82 percent. Also the citrate synthase activities of the leaves of T_0 progeny show 5～9 higher than the control lines.

转化过程中分别使用了960个Atlas66、1000个EM12和1200个Scout 66幼胚(总记3160个)，经抗性筛选、CS与标记基因的PCR检测初步表明有26株为阳性植株，总转化率为0.82%；同时进一步对T_0代植株叶片柠檬酸合酶以及T_1代植株经铝胁迫后对其根尖分泌物进行检测，结果在上述转化植株中检测到了柠檬酸合酶的活性，且转化材料比对照材料平均高出了5～9倍；同时铝胁迫后，T_1植株根尖检测到了苹果酸及柠檬酸的分泌。

ABSTRACT Objective: In order to study the mechanism of guttatim epidural injection , the experiment was designed to research into the digestion of collagens of nucleus after injection and the relationship between the rate of injection and the dose level of collagenase.

上海第二医科大学附属新华医院伤科，上海 200092 ； 2 上海市伤骨科研究所，现：上海沐阳医院）摘要目的：观察盘外置管法注射胶原酶是否有利于髓核的溶解以及胶原酶的注射剂量与滴速对髓核的溶解是否有影响。

The article summarizes the biological function and enzymology of protoporphyrinogen oxidase, site of action and mode of action of inhibitors of this class, their herbicidal effects, relationship of structure and activity, and weed resistance of PPO inhibitor

概述了原卟啉原氧化酶的生理功能和酶学特征，该类抑制剂的作用位点和作用方式，除草效果，构效关系研究及该类抑制剂的杂草抗性等问

A study on HPLC analysis of benzopyrene in grain;2. Induction of EROD activity was investigated by an in vitro assay based on benzopyrene and hexa chlorobenzene .

用7-乙氧基异叻唑酮-脱乙基酶检测的方法，研究了苯并芘和六氯苯对日本青鳉肝脏EROD酶的比活力的影响。

Compare to K type no.1B/1R male sterile line KTSP3314A,homotypic maintainer line TSP3314B and 1B/1R male sterile line K3314A,homotypic maintainer line 3314B at different developmental stages of superoxide dismutase,catalase,peroxidase activity and variety of malondialdehyde content.

利用K型非1B/1R类型小麦雄性不育系KTSP3314A，其同型保持系TSP3314B，以K型1B/1R类型小麦雄性不育系K3314A、其同型保持系3314B为对照，分别对其叶片和穗子在不同发育时期的超氧化物歧化酶、过氧化氢酶、过氧化物酶的活性和丙二醛含量的变化进行测定。

Hydroxamic acid β-glucoside is confirmed as a major natural substrate of maize β-glucosidase.β-glucosidase plays a significant role in defensing some pathogens and pests of maize seedling by releasing hydroximic acids .

并确认了氧肟酸葡萄糖苷即是玉米葡萄糖苷酶的主要底物，以及氧肟酸在玉米葡萄糖苷酶的催化下释放氧肟酸是玉米幼苗时期抵御病虫害的一种重要方式。

Chinensis and Kuruma prawn M. japonicus increased at 12 and 48 hours post-inoculum, but decreased sharply after 36 and 72 hours post-inoculum respectively. LPS inducible NOS activity also decreased significantly with the time elapsed in WSSV infected shrimps. Results of WSSV detection with the Dig-DNA Probe dot blot hybridization test showed that WSSV of the two penaeid species couldn't be detected until iNOS started decreasing.

在此基础上，我们建立了对虾血细胞中诱导型一氧化氮合成酶的鉴定方法—硝基四氮唑蓝还原法和血细胞形态法以及酶活力的测定方法—L-瓜氨酸分析法和亚硝酸盐分析法并优化了鉴定和测量条件，结果显示：对虾血细胞在4℃下与L-精氨酸和LPS孵育8h以及当L-精氨酸浓度为2.5mmol l〓LPS浓度为100μg ml〓，Ca〓浓度为2.5mmol l〓时，iNOS活力的测定结果最佳。

The immobilization of laccase is important for the fiber optic biosensor based on enzyme catalysis.

漆酶的固定化研究对基于漆酶催化的光纤生物传感器具有十分重要的意义。

The immobilization of laccase is important for the fiber optic biosensor based on enzyme cataly-sis.

漆酶的固定化研究对基于漆酶催化的光纤生物传感器具有十分重要的意义。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。