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Twenty varieties or materials of tomato with different resistance were used for experiment. Through testing the reaction condition and pH of extract of POD and PPO, identifying the genetic relationship of twenty varieties by POD isozyme and testing the biochemical indexes of resistance to ToMV, the main results were showed as follows:1. Classification of resistance: According to the campestral resistance to ToMV and the analysis experimental data ,the resistance of twenty varieties were divided into three kinds:z(1)resistant variety, including Zhong-za No9. Zao Hong-bao.

本试验以20个番茄品种或材料为试材，研究了过氧化物酶、多酚氧化酶酶液提取及反应适宜条件；应用POD同工酶对20个番茄材料进行亲缘关系的鉴定；对20份材料苗期室内接种ToMV后进行抗病性鉴定以及接种两周后所有材料POD、PPO、苯丙氨酸解氨酶的活性变化的研究，得出以下结论： 1。

Of the 4 soil enzymes studied, alkaline phosphatase and dehydrogenase were higher than urase and arylsulfatase in activity.

在所测定的4种土壤酶中，碱性磷酸酶和脱氢酶的活性高於脉酶和芳基硫酸酯酶的活性。

Experiment 4: Affection of Ginger Extraction on ATPase of Myocardium of Rabbit. Method Coomassie brilliant blue protein test-box and ATPase test-box were used to detect activities of Na〓-K〓-ATPase、Ca〓-ATPase and Mg〓-ATPase in the myocardium of rabbits, samples from the low-dosage, middle-dosage ginger group and 4-Retro-decoction group of the above"Experiment 3". A extra control group (10 rabbits) was established in this experiment.

干姜提取物对兔心肌ATP酶活性的影响方法采用考马斯亮兰蛋白测试合和ATP酶测试合对兔心肌Na〓-K〓ATP酶、Ca〓-ATP酶和Mg〓-ATP酶的活性进行检测，标本来源于上述"实验3"的低、中剂量干姜组和四逆汤组，并另设心衰对照组（10只兔）。

The strawberry fruit were sampled from 1 to 8 weeks after full blossom. The contents of sucrose, glucose and fructose and the activities of sucrose phosphate synthase, sucrose synthase, acid invertase, neutral invertase, hexokinase and fructokinase were measured in developing strawberry fruits.

采集花后1~8周的果实，分别测定果实中葡萄糖、果糖、蔗糖的含量和蔗糖磷酸合酶、蔗糖合酶、可溶性酸性转化酶、中性转化酶、己糖激酶及果糖激酶等蔗糖和己糖代谢酶的活性。

That means Pho85 kinaseand calcineurin were involved in salt tolerance with an identical target protein or in 中科院上海生化与细胞所博士学位论文摘要 the same pathway. Inhibition of calcineurin decrease the YPH499, pho80? mutant,and pap1 (pcl7)? mutant Mn2+ tolerance but not that of pho85? mutant and thepho85? mutant was more sensitive to Mn2+ than YPH499, pho80? mutant, and pap1(pcl7)? mutant even with the addition of cyclosporin A. Therefore, the conclusioncould be drawn that PHO85 gene played a dominant role in Mn2+ homeostasisregulation in compare with calcineurin. As for Ca2+ tolerance, cyclosporin A canincrease the tolerance to Ca2+ of all the mutant mention above, that means Pho85kinase and calcineurin function antagonistically in regulation of Ca2+ homeostasis.In Bioinformatics, BRI3 gene is a novel gene without any function clue but givehigh conservation in mammalian.

我们通过钙调磷酸酶的特异抑制剂环孢菌素A研究了YPH499、+2+pho85缺失株、pho80缺失株、pap1缺失株在钙调磷酸酶失活后对Na、Mn、2+Ca金属离子敏感性的变化，结果显示Pho85蛋白激酶和钙调磷酸酶通过直接+或间接激活同一个靶蛋白或途径来增强细胞对Na的耐受；和钙调磷酸酶相比，2+PHO85的缺失对酵母细胞Mn耐受性的破坏是相对控制性的，钙调磷酸酶的失2+活不能进一步降低pho85缺失株对Mn的耐受能力；Pho85和钙调磷酸酶在对2+Ca的耐受调节中是相互拮抗的，钙调磷酸酶的失活能增加pho85缺失株和2+pho80缺失株的Ca耐受。i中科院上海生化与细胞所博士学位论文摘要对本实验室克隆的人新基因BRI3进行系统的生物信息学分析，发现它是一个在哺乳动物中保守的，但功能未知的基因。

The zymogramatic variations of esterase isoenzyme and superoxide dismutase isoenzyme of 20 sample materials, including 5 species, 2 varieties, 1 multiploid of Isatis L. and 1 outgroup species, Coronopus didymus J. E. Smith, were analyzed with technique of polyacrylamide gel electrophoresis.

采用聚丙烯酰胺凝胶电泳，比较了菘蓝属5种2变种1多倍体品种及1外类群种共计20个样本的酯酶同工酶和超氧化物歧化酶同工酶的酶谱差异，并运用数量分类学的原理和方法对酶谱数据进行了聚类分析。

The total number of colony forming units of culturable bacteria and fungi were increased significantly(p.01 in transgenic sugarcane rhizosphere soil, but The total number of CFUs of culturable was not effected. No significantly higher CFUs of bacteria, fungi and actinomycetes resistant to kanamycinwere obtained in transgenic sugarcane soil.

结果表明：转基因甘蔗显著(p.05)降低了土壤脱氢酶的活性，提高了土壤的纤维素酶、蛋白酶和脲酶活性(p.01)，而对蔗糖酶和磷酸单脂酶没有影响；转基因甘蔗显著增加了根际土壤可培养的细菌和真菌数量，但对放线菌影响很小。

The technics of producing membrance for glucose and sucrose detecting were optimized. The best condition is: adding fragmentation liquid of the yeast which is breaked up in 450 times for three times, betaking mix enzyme which is prepared with 0.0036g glucose oxidase and 0.5ml kluyveromyces lactis β-galactosidase and 1.5ml distilled water, immobilizing mix enzyme through 2% glutaric dialdehyde for 1 hour.

优化了葡萄糖和蔗糖酶膜制作的工艺条件，结果表明，在以下条件：3层破碎液次数450次的破碎液，浓度配比为0.0036g葡萄糖氧化酶和0.5ml半乳糖苷酶用1.5ml蒸馏水溶解的混合酶液，固定混合酶的戊二醛浓度为2%，时间为1小时，酶的固定效果最为理想。

Kinetics constant Km of crude laccase and laccase-isoform during the culture of wood scraps, corn stalks and straw stalks the three solid states was studied.

在木屑、玉米秆和稻草秆三种固体秸秆基质上分泌漆酶活性、漆酶动力学参数米氏常数Km和漆酶同工酶的动态变化。

The maximum MTG activity in the culture broth was 5.04u/mL and increased by 21.8% compared with the control. With the addition of CTAB, pro-MTG was activated to become MTG. CTAB could enhance the production of pro-MTG by relieving the pr...

初步研究表明，CTAB的主要作用是促使谷氨酰胺转胺酶的酶原转化为成熟酶，因此，在发酵过程中添加适当浓度的CTAB，可使酶原快速、完全地转化为成熟的MTG，解除酶原的产物抑制作用，促进了细胞产酶。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。