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Mehtod: The animals were given intraperitoneally normal saline(10ml/kg.d), phenobarbital (40mg/kg.d) and puerarin (100, 200mg/kg.d) respectively for seven days. Then the levels of hepatic microsomal CYP and cytochrome b5(Cytb5), and the activities of aniline hydroxylase and aminopyrine N-demethylase were determined by spectrophotography.

实验动物分别腹腔注射生理盐水、苯巴比妥(40mg/kg.d)、葛根素(100和200 mg/kg.d),给药7d后,用分光光度法测定肝微粒体CYP和细胞色素b5(Cytb5)的含量,以及苯胺羟化酶和氨基比林N-脱甲基酶的活性。

Recombinant plasmid pFf-22-Trp operon was successfully constructed, and both the anthranilate synthase and tryptophan synthase activities of expressed product increased. It laid a foundation of construction of recombinant E. coli strain for production of tryptophan in a large scale.

已成功构建了重组表达质粒pET-22b-Trp operon,邻氨基苯甲酸合成酶和色氨酸合成酶的活性在大肠杆菌中得到了提高,为高产色氨酸基因工程菌的构建奠定了基础。

The activity of enzyme production of bacteria from the soil samples that from oil refinery was higher, and the best carbon source for bacteria fermenting and producing lipase was sucrose, and the best nitrogen source was beef extract.

结论]炼油厂土样中细菌的产酶活性较高,细菌发酵产脂肪酶的最佳碳源为蔗糖,最佳氮源为牛肉膏。

The quantity of product ADP before and after reaction which was determined by RP-PHLC was dependent on the PFK activity.

通过RP-HPLC测定酶反应前后产物二磷酸腺苷量的变化来分析酶的活性。

After sunrise, lift gradually along with air temperature, enzymatic vivid capriole comes, those who promoted flower is open, rise to certain level when air temperature, enzymatic active loses decreasingly gradually again, flower died of old age naturally also.

日出后,随气温逐渐升高,酶活跃起来,促进了花朵的开放,当气温上升到一定程度,酶的活性又渐渐减弱,花朵也就自然凋谢了。

Flow-injection spectrophotometry was established to measure carbonic anhydrase activity and the effect of magnetic field on CA activity was studied.

建立了流动注射分析方法,评价了碳酸酐酶的活性,并用此方法研究了磁场对酶催化活性的影响。

The kinetic analysis indicated that one tryptophan and one carboxyl group in one molecular of XynA were essential for the XynA activity.

XynA的化学修饰动力学分析表明,每个酶分子中有 1个色氨酸残基和 1个谷氨酸残基对酶的活性特别重要。

It was investigated that the immobilized catalase was affected by temperature and pH and inhibited by hydrogen peroxide.

研究了温度和pH值对酶活性的影响及过氧化氢和尿素对固定化酶的抑制作用。

Improving the activity of cellulase by genetic engineeringmethod is a efficient way to cut down the cost price.

通过基因工程手段,提高纤维素酶的活性是降低纤维素酶成本的一个有效途径。

In this thesis, we studied the effects of nuclear actin on gene transcription regulation, with a special focus on the relations between the nuclear actin and RNA polymerase II in concanavalin A-stimulated lymphocytes and HeLa cells.

本文以ConA激活的淋巴细胞和HeLa细胞为实验材料,从不同角度研究了细胞核肌动蛋白与RNA聚合酶Ⅱ相互作用,并对细胞核肌动蛋白参与RNA聚合酶Ⅱ的作用机制进行了探讨。

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