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The effects of pH value on production of cellulase from Trichoderma viride were investigated.

以绿色木霉为产酶菌,研究了不同pH值控制方法对绿色木霉合成纤维素酶的影响。

Objective: To investigate the inhibitory effect of a new highly se lective inducible nitric oxide synthase inhibitorNGnitroargininelysine tripeptide on cNOS and iNOS indu ction .

目的:研究硝基精氨酸赖氨酸三肽对组成型一氧化氮合酶和诱导型一氧化氮合酶的抑制程度。

The optimal chitinase fermentation conditions of strain SWCH-6 were conformed by single-factor experiments and orthogonal experiments, they were colloidal chitin 25.0 g/L, tryptone 10.0 g/L, seawater 1 L, pH 8.5, 32℃, 150 r/min for 72 h.

采用单因素优化方法结合正交实验,得到菌株SWCH-6产几丁质酶的最佳发酵条件:胶体几丁质25.0 g/L,胰蛋白胨10.0 g/L,陈海水1.0 L, pH 8.5, 32℃, 150 r/min培养72 h;在该条件下酶活力达0.39 U/mL。

The production and characterization of endoglucanase from Streptomyces xylophagus KX6 was studied. Maximum endoglucanase yield of 0.538 IU/ml was achieved with medium pH8.0, containing CMC2Na 1.0% as carbon resource, soybean meal 1% as nitrogen resource, 2% inoculating volume, 30% 250 ml triangle flask bulk for medium volume at 40℃ 200r/min shaker for 48h.

实验中对其产酶的液态发酵条件进行了研究,碳源为1%羧甲基纤维素钠,氮源为1%豆粕粉,250ml三角瓶30 %装液量,接种量为2%,培养基初始pH为8.0,培养温度为40℃,200r/min培养48h后,发酵液中内切葡聚糖酶活达到0.538IU/ml。

Under treatment condition from 500MPa to 900MPa, activities of both enzymes were completely inact...

在室温350-850MPa施压处理条件下,纤溶酶和脂肪酶的最低钝化压力均在450-500Mpa间,最高钝化施加压力分别为850MPa和750MPa。

In the reasonable drug molecular design,analyzing the conformations of the substrate without three-dimension structure and confirming its active conformation are very important to illustrate the spatial shape and active site of target enzyme. The active conformation of protoporphyrinogen IX substrate of protoporphyrinogen oxidase has been established using the Distance Comparisons DISCO method. In this paper, four different conformational molecules of Protogen IX and crystal molecule of diphenylethersAF are analyzed by comparing their geometry parameters. The results show the lower energy active conformation from the DISCO model is more reasonable.

在合理药物设计方法中,当靶标酶的三维结构未知时,对其底物进行构象分析,特别是确定其活性构象,对阐明靶标酶活性中心的空间形状和作用位点具有十分重要的意义我们曾利用距离比较法确定了原卟啉原氧化酶的底物—原卟啉原IX的活性构象,本文从构象分析的角度对4种不同构象的原卟啉原IX分子与二苯醚类分子的晶体学构象进行了几何参数的比较和分析,结果进一步证实了距离比较法所确定的活性构象更为可靠

Ca2+-ATPase and acid phosphatase of fiber in Phyllostachys edulis culms during secondary wall formation were studied. Early, intact double karyotheca remained in fiber nucleus, while nuclear chromatin agglutinated.

利用显微和细胞化学方法,对毛竹茎秆纤维次生壁形成过程中超微结构变化以及ATP酶、Ca2+-ATPase和酸性磷酸酶的超微细胞化学定位进行了研究。

An enzyme that catalyzes the addition or removal of water from a substrate.

外酶酶的一种,如消化酶,在细胞外发生作用

Inactivation of the enzyme folioed an elhance of GndHClconcentlation. Half of the ovelall reaction activity was lost within l.5 Ininuthe in 0.54Inol/LGndHO, and 5% achvity remained at the end of inachvation.

在低浓度盐酸胍溶液中该酶的全反应活性和其中两个还原部位单独活性被同步可逆抑制,随着胍浓度增高,出现不可逆失活且程度和速度均迅速提高,在0.54mol/L胍中该酶全反应活性在1.5分钟内已有一半不可逆丧失,终态只余约5%活性。

An enzyme that hydrolyzes peptides into amino acids.

和辅酶结合构成活性酶的蛋白质。

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