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As to 1,3-propanediol oxidoreductase, the Km value for NAD and 1,3-propanediol are 0.15 ±10-3 M and 0.97±10-3 M respectively.

最后,本文考察了甘油脱氢酶和1,3—丙二醇氧化酶的动力学性质。

The specific activity of pectate lyase was 15 U/mg crude protein.

细胞经超声破碎后,测得果胶酶的比酶活为15U/mg粗蛋白。

XN also did not have any influence on mRNA expression of the enzymes CYP1A2 and quinone reductase.

同时黄腐酚对酶CYP1A2和醌还原酶的基因表达也没有任何影响。

Reciprocal equation.

Wilson改良双倒数方程数据处理法进行数据处理,测定天然酶与重组酶的Km、Vmax及kcat。

Effects of mannitol and 6 BA on the rice cell redifferentiation,activities of peroxidase and IAA oxidase were investigated.

研究了甘露醇和 6- BA处理对水稻悬浮细胞再分化、过氧化物酶及 IAA氧化酶的影响。

And the enzymatic characteristics of 3-mannanase was determined by using single factor analysis method.

方法]运用单因子分析对β-甘露聚糖酶的酶学特性进行研究。

ASQS is 70%, 77%, 44% and 39% identical to squalene synthases from arabidopsis thaliana, tobacco, human and yeast, respectively.

青蒿鲨烯合酶氨基酸序列与拟南芥、烟草、人类、酵母鲨烯合酶的一致性分别为70%、77%、44%、39%。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识,会用显微镜检测观察微生物,培养基的制备、消毒与灭菌,分离与纯化微生物,细菌的计数法具备分析化学的理论知识,能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理,了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识,PAGE凝胶电泳技术,高速离心掌握基因工程的基本理论知识,基因的提取和纯化,PCR技术原理,基因重组技术掌握酶的基本理论知识,酶的固定化方法,酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器:天平(台天平,电子天平,分析天平)、酸度计、分光光度计、液气相色谱仪,酸碱滴定仪器,显微镜,离心机,超声波清洗器、恒温振荡培养箱、超净工作台,蒸气灭菌锅、恒温水浴锅,PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

ABSTRACT Bacillus subtilis tryptophanyl-tRNA synthetase was expressed and purified by the aid of the high-level expr...

利用枯草杆菌色氨酰tRNA合成酶的高表达质粒pKSW1表达和纯化了枯草杆菌色氨酰tRNA合成酶。

On Day 5, the spinal cords were removed and prepared for Western blot analysis of glutamate dehydrogenase and glutamate synthetase.

第五天,取髓作谷氨酸脱氢酶和合成酶的 Western 印记分析。

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