酶的

This paper elaborates on the concept of directed molecular evolution, procedure, basic strategies, and its core technology, then presents emphatically its application and achievements in improving enzyme activities, stability, substrate specificity, and antipode selectivity and so on.

本文详细综述了酶的分子定向进化的概念、过程、基本策略及其核心技术，并着重介绍了酶的分子定向进化技术在提高酶的活力、稳定性、底物特异性和对映体选择性等几方面的应用及取得的相关成果。

Phytase was a kind of enzyme for hydrolyzing phytate to release myoinositol and inorganic phosphate, which would promote the utilization of phosphate, reduce the drainage of phosphate in the dejecta and reduce the environmental pollution.

植酸酶是催化植酸及植酸盐水解成肌醇与磷酸的一类酶的总称。植酸酶添加在饲料中，会将其中的植酸水解成肌醇和磷酸，从而提高了磷的利用率，降低了粪便中磷的排放量，减少了植酸磷对环境的污染。

Purified GO could oxidize both glycolate and glyoxylate, and both activities highly depended on FMN. The activity of glyoxylate oxidation was weaker than that of glycolate. During purification or storage of purified GO, the ratio of glycolate to glyoxylate oxidation was progressively enhanced.

纯化的菜心叶片乙醇酸氧化酶对乙醇酸和乙醛酸均有氧化活性，且两者都强烈依赖于FMN，但对乙醛酸的氧化能力相对较弱；在酶的纯化及纯化酶的贮存过程中其两催化活性的比值均呈逐渐升高的趋势。

Thenard(1811), the discoverer of hydrogen peroxide, expected that its degradation in living tissue is performed by a special substance, which Schonbein (1863) identified as a certain kind of 'ferment'. Loew (1901) was the first who named this H2O2-degrading enzyme catalase, but it took further 22 years until Warburg (1923) demonstrated that the active center of catalase contains iron, as concluded from the characteristic inhibition with cyanide.

过氧化氢的发现者Thenard ，希望活体组织中过氧化氢的降解是由一种特殊物质引起的，Schonbein定义这种特殊物质为某种酵种，Loew是第一个定义为过氧化氢降解酶，22年后Warburg 证明过氧化氢酶的活性中心含有Fe，正如从已知氰化物的特性得到结论。1927年，WIeland提出了一个简单的细胞过中氧化氢酶的解释。

Through screening lipases, lipase Novozym 435 and lipase Alcaligenes sp were found to show higher activity and better enantioselectivity for the resolution of 2-(2-chloro- 1-hydroxyethyl)thiophene. The optimal reaction conditions were finally achieved by further investigating the effects of solvents, temperature, rotation speed and enzyme concentrations.

通过对不同来源酶的筛选，找到了Novozym 435和Alcaligenes sp两种选择性较好的酶，它们均对该反应具有较高的选择性和较快的反应速度，在此基础上进一步通过对溶剂、温度、摇床转速以及酶用量的筛选，确定了能够有效拆分2-氯-1-(2-噻吩)-乙醇的较佳反应条件。

The catalytic action of various kinds of functional coordination compound and metallomicelles which are made from the complex and micelle, as mimic hydrolase or peroxidase, have been studied respectively in catalyzing the carboxylic ester and phosphatic diester hydrolysis, and the phenolic derivant oxidation in this paper. Some novel results of academic significance have been obtained.

本文以不同类型的功能配合物及其与表面活性剂形成的金属胶束作为水解酶和过氧化物酶的模拟酶模型，分别研究它们对羧酸酯、磷酸二酯水解反应和酚类氧化反应的催化作用，取得了新的有学术意义的成果。

In the same time, using Yang Mai 5 as experiment materials, the effects of ABA and Zeatin on membrane physiology, important photosynthetic parameters, key enzyme of photosynthetic C〓 cycle and proteas, nuclease were also studied.

本文以杨麦5号，杨麦158，麦优4号为材料研究了根系功能和叶片光合功能的变化，对伤流液和根、叶中的ABA、ZRs做了系统的分析测定；以杨麦5号为材料对两种激素在膜生理，重要光合作用参数，光合C〓循环关键酶及蛋白质水解酶，核酸酶的作用进行了研究。

The seedling height and the leaf area increased after treatments.3 Rape seeds were cultured in the solution containing diosgenin of different concentrations. The different concentrations of diosgenin affected metabolism of rape seedlings, particularly at a concentration of 0.5mg/L. The treatments of 0.5mg/L diosgenin increased prominently the contents of soluble sugar, soluble protein and the activity of peroxidase, catalase, Ca2+- ATPase in rape cotyledons.4 Rape seedlings were sprayed with 0.5mg/L diosgenin solution at the two-leaf and one-bud stage.

油菜种子经不同浓度的薯蓣皂素浸种处理后，0.5 mg／L的薯蓣皂素明显影响油菜幼苗生理代谢，子叶中可溶性蛋白质和可溶性总糖含量提高，过氧化物酶和过氧化氢酶的活性，Ca~(2+)-ATP酶的活性上升。

The morphological changes in the steam-treated straw were characterized by scanning electron microscopy. It is proposed that the use of the ball mill shaker both reduces the degree of inhibition of the enzyme by reaction products and increases the rate of adsorption and desorption of the enzyme on the substrate.

用环境扫描电镜观察三种不同方式酶解后的汽爆麦草的形态，经分析发现，钢球振荡还有助于减少产物对酶的抑制和提高底物对纤维酶的吸附和解吸频率。

The effect of Tween 80 addition, enzyme loading and substrate concentration on the re-adsorption of cellulases, which hydrolyzed the corncob after the pretreatment by soaking in aqueous ammonia, was studied in this paper.

论文通过实验方法，考察了重吸附法回收纤维素酶中的一些因素（表面活性剂浓度、加酶量和底物浓度）对回收利用效果的影响规律，得到了较优的工艺参数范围，对于解决纤维素酶的回收利用有一定参考价值。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。