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The toxical effects of nonylphenol ethoxylates and nonyiphenol were studied with EROD in liver of carp as biomarker under external condition. These two tested substances all had activation on EROD enzyme in liver of carp. Under definite range of concentrations, with the affecting rule of inhibiting at high concentration and inducing at low concentration.

在离体条件下,以鲤鱼肝脏EROD酶为生物标志物研究了壬基酚聚氧乙烯醚及壬基酚的毒性效应结果表明,在一定的浓度范围内,2种受试物对鲤鱼肝脏EROD酶均有激活作用受试物浓度对EROD酶的活性的影响规律为高浓度时抑制,低浓度时诱导。

It is caused by mutations in dyskerin-encoding genes, telomerase-encoding RNA component genes and reverse transcriptase genes, as well as other uncharacterized genes. There are three inherited forms, including X-linked, autosomal dominant and autosomal recessive inheritance.

先天性角化不良是与编码角化不良蛋白基因、编码端粒酶的RNA组份基因、编码端粒酶的逆转录酶基因突变及其他未确认的致病基因突变引起的基因病,其遗传方式有:X-性联隐性遗传、常染色体显性遗传及常染色体隐性遗传。

Two molecular forms of Xenopus hatching enzyme, 60kD and 40kD molecules, was obtained during preparation and purification. Both of them were verified as the hatching enzyme molecules, using anti GST UVS.2 antibody as the probe. 60kD molecule was digested or autodigested easily into 40kD molecule during purification. It was indicated that 40kD molecule was probably derived from 60kD molecule with its two CUB repeats lost, and the two CUB repeats may play an important role in recognizing and/or modifying of the molecular structure of vitelline envelope.

在分离纯化非洲爪蟾孵化酶时,得到了60kD和40kD两种分子,用孵化酶的特异性抗GST-UVS.2抗体进行Western杂交的结果证明二者均为孵化酶分子。60kD分子很不稳定,在纯化过程中极易降解,40kD分子可能是由60kD分子经过降解或自身降解丢失了两个CUB重复区而形成的,而CUB重复区很可能在对受精膜分子结构的修饰或改造中具有重要作用。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group).The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group).The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组(K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组),采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量,流式细胞术检测细胞内阿霉素的浓度,基因、酶学、蛋白水平检测实验分3组(K562组、K562/A02组和K562/A02+TTD组),采用RT-PCR法检测mdr1 mRNA的表达,免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平,Western-blotting法检测P-糖蛋白和bcl-2表达。

However,it has its original catalyzing functions with high efficiency,specificity and moderate reaction condition.

酶的固定化是用人工方法把从生物体内提取出来的酶固定在特定的载体上,酶被限定在一定区域内,但仍保持其原有高效、专一、条件温和的催化功能。

Long period illumination could lower the activity of cytochrome oxidase in tissues, so that this enzyme activity in green leaves was much lower than that in etiolated leaves. The activity of isolated cytochrome oxidase, however, was not affected with light of varied intensities.

虽然光的长期作用能降低细胞色素氧化酶活性,使绿叶中该酶的活性大大低于黄化苗,但各种强度的光对提取的细胞色素氧化酶活性没有影响。

The results of the crystal structure indicated that the deletion of 8 amino-acid residues from the C-terminal end of SNaseR perturbs the conformation of residue Tyr113 and blocks the binding between enzyme and substrate, which causes the biological activity of SNR141 down to 80%of wild type nuclease.

晶体结构的结果表明,从SNaseR C端切除8个残基后,扰动了残基Tyr113的构象,妨碍了酶与底物的结合,使N端片段SNR141的生物酶活力下降为天然酶的80%。

This paper summarized the property, kinds and mechanism of the rejuvenation and advances of its research on tenderizing enzyme. The newest applications advances tenderizing enzyme of and open questions in process of animal product and trends of vary tenderizing enzy...

对肉类嫩化酶的种类、性质、嫩化机理以及国内外的研究现状进行综述,探讨了不同肉类嫩化酶在畜产品加工中的最新应用进展及存在的问题,并对肉类嫩化酶的发展趋势作一展望。

The major classes of detergent enzymes-proteases,lipases,amylases,and cellulases-each provide specific benefits for application in laundry and automatic dishwashing.

洗涤剂酶的主要类别(蛋白酶、脂肪酶、淀粉酶和纤维素酶)中的每一类为洗衣和自动洗碗机的应用提供了专门的好处。

Apoenzyme An ENZYME whose cofactor or prosthetic group has been removed (e.g. via dialysis) rendering it catalytically inactive. It is the protein part of a conjugate enzyme.

脱辅基酶蛋白:酶的辅助因子或辅基去除后剩下的没有催化活性的部分,是共轭酶的蛋白质部分。

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