酶的

The activities of carboxylesterase、glutathion-S-transferase and acetylcholinesterase per insect increase greatly and the properities of esterase and acetylcholinesterase change also throughout larval development from 1-day-old to 4th instar. The affinity for Asch and the sensitivity to insecticide of acetylcholinesterase in 3rd instar larvae are significantly lower than that in 4-day-old larvae.

幼虫从1日龄生长到4龄时，单头幼虫的羧酸酯酶、谷胱甘肽转移酶、乙酰胆碱酯酶活力急剧增长，4龄幼虫相对于1日龄幼虫，HJ-R、HD-S上述3种酶系的活力分别增长了148、172.6、65.6倍和203、60.9、77.4倍；生长过程中，全酯酶、乙酰胆碱酯酶的性质也发生变异，特别是从4日龄生长到3龄时，乙酰胆碱酯酶的米氏常数、双分子速率常数均发生明显的改变，3龄幼虫乙酰胆碱酯酶对底物的亲和力以及对杀虫剂的敏感性显著低于4日龄幼虫。

It includes determination of properties of the acid protease and lipase, application of acid enzymes to processes of bating pickling pelt and wet blue of sheepskins, analysis of activities of collagenase in acid protease and trypsin. Comparison was conducted to acid bating and common bating. Orthogonal experiments were used to determine influence of some factors in acid bating on qualities of leather. Scanning electronic microscope was used to observe separating of collage fibers.

对酸性蛋白酶、酸性脂肪酶的性能进行了测定；将其用于绵羊服装革生产的酸性软化及蓝湿皮软化中，利用正交试验法研究了酸性酶软化各因素对成革质量的影响，并与常规酶软化进行了对比；用扫描电镜观察了酸性酶软化后胶原纤维的分散情况；对酸性蛋白酶的分子量进行了测定，对比了酸性蛋白酶与胰酶中的胶原酶活力，以说明酸性酶软化与胰酶软化对胶原性质的影响。

Glucosidase and 3-dehydrogenase of HCCB 20049-2 are induced by sugar. After studying the relationship within inducing factors、enzyme activities and transformation rate, it was found that when cellobiose was used as inducer, enzyme activities and transformation rate were both higher than other sugars.

HCCB 20049 菌中β-葡萄糖苷酶及3-脱氢酶均为糖类诱导酶，对这两种酶的诱导因子与酶活力和有效霉素的转化率的关系进行研究，发现纤维二糖诱导的β-葡萄糖苷酶和3-脱氢酶活力都较高，其valienamine 的生成率最高。

METHODS: A limb ischemia-reperfusion injury model in rats was established. The animals were randomly divided into three groups: control group, IR group and IPC group. The contents of diamide oxidase, nitric oxide, endothelin-1 (ET-1) and ratio of nitric oxide/endothelin-1 (NO/ET-1) in the plasma and the gut were measured. The leavels of myeloperoxidase, ratio of DNA chain, total nitric oxide synthase, inducible nitric oxide synthase and constitutive nitric oxide synthase in the gut were determined.

雄性Wistar大鼠18只，随机分为对照组，缺血再灌注组和缺血预适应组，每组6只，分别测定血浆和小肠组织二氨氧化酶、一氧化氮、内皮素-1(ET-1)、NO/ET-1比值的含量变化及小肠组织的髓过氧化物酶、DNA双链百分率(ratio of DNA chain %)、总一氧化氮合酶、诱导型一氧化氮合酶、结构型一氧化氮合酶的水平；免疫组化法检测小肠组织的诱导型一氧化氮合酶、内皮型一氧化氮合酶的表达。

This article focus on enzyme gene clone and heterogenesis expression,site directed modify and orthogenesis,fu-sion protein and fusion enzyme,enzyme mimics(abzyme and molecular imprinting)and telomere .

本文着重介绍了酶基因克隆与异源表达、酶分子的定向改造和进化、融合蛋白与融合酶、酶的人工模拟和端粒酶，综述了分子酶工程的研究进展、趋势及其应用。

The activities of catalase, polyhenol oxidase, urease, protease, saccharase of grafted eggplants were higher than those of the control, but the activities of peroxidase and phosphatase were obverse.

嫁接茄子的过氧化氢酶、多酚氧化酶、脉酶、蛋白酶、蔗糖酶的活性均高于自根茄子，但过氧化物酶和磷酸酶的活性自根茄子高于嫁接茄子。

The most appropriate catalyzed temprature and pH for it is 60 ℃ and 5.5 respectively. It has the extreme stability at the temperatures no more than 50 ℃.The CMC-Na has the protective effect on the cellulase. The pH stability range of the purified cellulase is 4 ~ 8. Zn2+, Ca2+, Mg2+, K+, Li+ can ativate the cellulase. And cellulase can mainly decompose CMC and salicin, but can decompose other substrates feebly.

对这个组分的催化性质进行了研究，该酶的最适催化温度60 ℃，pH值为5.5，在50 ℃以下酶的稳定性较好，70 ℃条件下底物对酶有较强的保护作用，该酶的pH稳定性范围为4～8， Zn2+，Ca2+，Mg2+，K+，Li+对酶活有促进作用，Mn2+、Co2+、、Fe2+、Fe3+对酶活起抑制作用，酶对CMC-Na和水杨素有分解作用，而不分解脱脂棉和滤纸。

Based on the absorbance change of indicators with the concentration of hydrogen ion released from the enzyme-catalyzed reaction, a convenient colorimetry method is established for the assay of acidic phospholipase 〓 and glycogen phosphorylase b Brilliant yellow and bromothymol blue are chosen as indicators for assays of acidic phospholipase 〓 and glycogen phosphorylase b by following the absorbance changes at 495 nm and 615nm respectively The method is simple, sample-saving, sensitive and valid for a wide range of enzyme concentrations.

为了研究糖原磷酸化酶的激活动力学和酸性磷脂酶〓的复性过程，我们根据酶催化反应中释放氢离子浓度的变化引起相应的指示剂的光吸收发生变化的原理，建立了一种简捷的比色法，用于测定酸性磷脂酶〓和糖原磷酸化酶b的活性。选用亮黄和溴麝香草酚兰分别作为酸性磷脂酶〓和糖原磷酸化酶b测活的指示剂，在495 nm和615 nm处检测二者的光吸收值的变化，可以测定酶活。本方法的优点是，可在比较宽的酶浓度范围内进行活力测定，而且操作简单、节省样品、灵敏度高。

ASES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi-cedrol synthase from A. annua, 48% identical to amorpha-4, 11-diene synthase from A. Annua, 39% identical to the 5-epi-aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. Muticus, 41% identical to the δ-cadinene synthase from cotton. The coding region of cDNA was cloned into procaryotic expression vector pET30a and overexpressed in E. coli BL21 (DE3). The cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction. The tissue specific expression patterns were analyzed by RT/PCR.

克隆的倍半萜合酶氨基酸序列与烟草马兜铃烯合酶、莨菪岩兰螺旋二烯合酶、棉花杜松烯合酶的一致性分别为39％，38％和41％；与青蒿柏木脑合酶、紫穗槐二烯合酶和一个推测的倍半萜合酶克隆cASC125的一致性为50％，48％和59％。cDNA编码区序列被克隆进原核表达载体pET-30a，并在大肠杆菌BL21（DE3）中诱导表达，但过量表达的蛋白主要是以不溶性蛋白形式存在。

Progress of Research and Development of Chymosin and Its Substitutes in China;2. The research developments of substitute s of chymase,such as animal chymase,plant chymase,microorganism chymase and genetic engineering chymase,are also discussed.

为了发展我国的干酪产业，阐述了干酪生产的现状，凝乳酶的结构和凝乳机理，影响凝乳酶活性的因素为pH值、温度、Ca2+及其它因素，并介绍了国内凝乳酶代替品动物性凝乳酶、植物性凝乳酶、微生物性凝乳酶和基因工程凝乳酶的研发进展。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。