酶的

Methods Ampicillin inhibitionof E. coli Top10 pcs+ was tested at first, and then b-lactamase activity in periplasm was examined.

然后再使用抗生素抗性分析、b-内酰胺酶的酶活测定以及Western blot杂交技术，分析质粒编码的b-内酰胺酶从细胞质到细胞间质的分泌情况。

The phytotoxin can restrain the activities of peroxidase , polyphenol oxidase and phenylalanine ammonia lyase that existed in the tree, and so reduce the resistance of the host tree and benefit the fungus to intrude into the tree successfully.

该毒素能抑制寄主组织中过氧化物酶、多酚氧化酶和苯丙氨酸解氨酶的活性，降低寄主树木的抗御能力，从而有利于菌突破寄主的生理防御屏障而成功地侵染生长。

In situ hybridization and immunocytochemical studies indicated that treatment with 17beta -estradiol increased the number of aromatase-immunoreactive cells and their immunoreactivities, especially in the preoptic area of the hypothalamus, where the density of the aromatase-immunoreactive cells was 2.6 folds higher than the control group. Results indicated that brain aromatase may be involved in the feminization induced by 17beta -estradiol of the grey mullet.

原位杂交和免疫细胞化学定位表明：雌二醇处理组鲻鱼脑内芳香化酶阳性细胞数量和反应强度均提高，尤其在下丘脑视前区，芳香化酶阳性细胞的分布密度为对照组的2.6倍，说明17beta-雌二醇诱导幼鲻雌性化可能与脑芳香化酶的表达有关。

Results showed that the specific activity of nitrile hydratase per dry cell weight in engineered Rhodococcus strains driven by Ptac, Pami-1, Pami-2 and PlacZ was 7.5, 6.3, 5.3 and 1.8 times of that in the wild, respectively. It indicated that these promoters could be well recognized by RNA polymerase of Rhodococcus.

结果表明，启动子Ptac、Pami-1(7 bp SD-ATG间隔)、Pami-2(13 bp SD-ATG间隔)和PlacZ在紫红红球菌ATCC 33278中启动表达腈水合酶的比酶活分别是野生菌株的7.5、6.3、5.3和1.8倍，表明这些启动子都可以被红球菌的RNA聚合酶所成功识别。

5 Compared stability of tannase in RM with that of free tannase in aqueous, tannase is more stable in RM than in aqueous.

2.5 比较反胶束体系中单宁酶的稳定性和水相游离酶的稳定性试验结果说明：反胶束体系有利于单宁酶的稳定。

We detected the distribution of lipases in 35 Aeromonas isolates by PCR andphenotypic test using tributyrin as substrate. The positive rates were 100% and 83%,respectively. The lipase activity or associated genes could be detected in the two avirulentstrains, A. hydrophila W1 and A. hydrophila 4332. It suggested that lipase might not be animportant factor in pathogenic mechanism of A.

对本实验室保存的35株气单胞菌分离株进行了脂酶的表型检测和PCR检测，结果分别为83％和100％，特别是嗜水气单胞菌无毒株W1和Ah4332也能检测到脂酶活性或基因，表明脂酶在该菌的致病机制中不是决定性因子。

By now, there is seldom report on the Heilongjiangwood frog which hibernates at low temperature condition. Our study mainly analyze theadaptation changes of liver proteins, SOD, LDH, G6PDH and EST activities of Heilongjiangwood frog under low temperature.

本文旨在研究低温条件下黑龙江林蛙肝脏蛋白质、超氧化物歧化酶、乳酸脱氢酶、葡萄糖6-磷酸脱氢酶(G6PDH)及酯酶的适应性变化，以探讨黑龙江林蛙冬眠期低温耐受的机制，为两栖类低温生物学和比较生理学的深入研究提供理论参考和实验依据。

To master the isolation of enzyme in cell .the concept ,mechanism ,the significance of Allosteric regulation of key enzyme .

掌握：细胞内酶的隔离分布；关键酶的变构调节—概念、机制、生理意义；酶的化学修饰调节—概念、特点。

AtDFA is dihydrofolate synthase localized in mitochondria, it catalyzes one glutamate added to the dihydropteroate and produces dihydrofolat. AtDFB, AtDFC and AtDFD are folylpolyglutamate synthase localized in plastid, mitochondria and cytosol respectively. A short chain ofγ-linked glutamates can be added by FPGS.Folate molecules exist in vivo mainly as polyglutamates and these are preferred by folate-dependent enzymes.

AtDFA为二氢叶酸合成酶(dihydrofolate synthase，DHFS)，定位于线粒体，在它的催化作用下，一个谷氨酸分子与二氢蝶酸(dihydropteroate ， DHP)相结合，形成二氢叶酸；AtDFB、AtDFC、AtDFD为多聚谷氨酸合成酶(folylpolyglutamate synthase，FPGS)，分别定位于质体、线粒体和细胞质，在多聚谷氨酸合成酶的作用下，谷氨酸分子可以逐一连接到四氢叶酸的谷氨酸γ位。

We can find that the conformational change of active site ofRubisco is faster than that of holoenzyme.

比较酶的失活与构象变化，发现酶的活性中心构象变化先于整个酶分子的构象变化。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。