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Chymosin was expressed in Pichia pastoris successfully, and a strong band at about 37 kD was shown by SDS-PAGE. Activity tests showed that the chymosin activity of the culture supernatant was 12.2 SU/mL.

在甲醇诱导下进行凝乳酶的表达, SDS-PAGE分析证明重组凝乳酶的分子量约为37 kD,培养基上清液中凝乳酶的活性为12.2 SU/mL。

The effects of situ hybirdization study show that the expression of NO-i increases evidently in the cytolymph of bone. It proves that GSL can obviously increase the content of NO-i and promote the synthesis of bone and secretion of iNOS.

组化及原位杂交研究结果显示,在软骨细胞的胞浆中,NO合成酶的表达显著增加,表明骨疏灵能明显增加NO合成酶的含量,并能促进成骨细胞合成和分泌NO合成酶。

Results The in vitro coupled bacterial luciferase:FMN-NADH oxidoreductase bioluminescent system was: 1mL crude extract, 27 mmol/L Dodecane 100 μL, 10 mmol/L FMN-Na 0.5 μL and 0.14 mmol/L NADH 300 μL.

利用从鳆发光杆菌提取并经部分纯化的荧光素酶和FMN-NADH氧化还原酶,通过优化体系中各底物的添加量,实现荧光素酶的体外发光。

The six stages involved euthermic state (25℃), prehibernation (10-12℃), after hibernating for a week and a month (2-4℃), and arousing from hibernation for a week and two weeks (10-12℃). From euthermic state to long-term hibernation, lipid peroxidation was showed to increase in brain except the stage of prehibernation.

分别测试了上述各阶段荒漠沙蜥1%脑组织匀浆、0.5%心脏组织匀浆及1%肝脏组织匀浆中:丙二醛及过氧化氢(H_2O_2)的含量,超氧化物岐化酶、过氧化氢酶、谷胱甘肽过氧化物酶的活性,及总抗氧化能力的水平。

Results1. Preparation of rude extracts of Lumbricus Bimastus nucleases and its purification Two groups of nucleases with different characters lied in the DNA-casting SDS-PAGE.

结果1。双胸蚓组织中核酸酶粗提物的制备及核酸酶的纯化应用本室建立的SDS-PAGE-DNA功能胶测活方法对核酸酶提取过程进行监测。

The HE activity was almost completely inhibited by SBTI, p-APMSF, bestatin and NEM, greatly inhibited by ovomucoid, TLCK, IAM, chymostatin and PMSF, and slightly inhibited by pepstatin A, TPCK, LBTI and leupeptin. And these results imply that HE is most probably a trypsin-type serine protease.

抑制剂对其酶活性的影响结果显示,该孵化酶的活性几乎可被SBTI、p-APMSF、Bestatin和NEM所完全抑制,被ovomucoid、TLCK、IAM、chymostatin和PMSF所强烈抑制,而对pepstatin A、TPCK、LBTI和leupeptin不敏感,表明该酶极可能是一种属于胰蛋白酶类型的丝氨酸蛋白酶。

Compared with the control, majority treatments could promote the activity of hemicellulase, and the effect on the activity of pectinase was less than that of other two enzymes.

与对照相比,多数药剂处理后能促进半纤维素酶的活性,同时药剂对果胶酶活性的影响不如对其它两种酶活性的影响明显。

The usage of mechanism exposition the pectinase and cellulase for textile was especially discussed in detail.

针对亚麻纤维织物的这一缺陷概括介绍了生物酶的种类、特性及作用机理,特别对纺织用果胶酶和纤维素酶的使用情况进行了论述。

The cellulose and the pectinase were proved better by comparing the results of different enzymes in degradation of coconut shell polysaccharides.

经比较不同的酶对椰壳多糖的降解,证实纤维素酶和果胶酶的降解效果较好。

Pectinase including many enzymes was wildly adopted in food industry.

果胶酶是分解果胶质的多种酶的总称,是一种在食品工业上广泛使用的酶。

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