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Then, the specific activities of protease and amylase were relatively lower than pancreatin separating with acetone, but the specific activity of lipase was higher. The ratio of activities in filtrate were very low, showing the precipitation was effective.

其中蛋白酶和淀粉酶的活力较低,与丙酮胰酶(3159U/g和58.7kU/g)相比有较大的差距,但是脂肪酶活力较高,高于丙酮胰酶的50.10kU/g。

2 A kind of enzyme and mediator modified electrode is devised by immobilyzing both N-methyl phenazine sulfate and glucose oxidase on the surface of carbon glassy electrode (GOD-NMP-GC electrode), which successfully realizes the electron transfer process between glucose oxidase and carbon glassy electrode.

将NMP-GC电极与葡萄糖氧化酶结合,制作了GOD-NMP-GC电极,该电极可快速实现电极=NMP介体=GOD活性中心=葡萄糖之间的电子长程转移,并且用循环伏安法对酶催化反应进行了研究;用含丰富乳酸脱氢酶的乳酸杆菌与NMP-GC电极相结合,制作了介体微生物电极,并检测到GC电极=NMP=LDH活性中心=乳酸之间的非均相电子转移。

This may be because that the metal ions in calf intestine AKP is more close to the surface of enzyme molecule than in Ulva pertusa kjellm , EDTA can exerted inhibitory effect on the AKP from calf intestine more easily and quickly.

这可能是由于小牛肠碱性磷酸酶活性位点的金属离子相对于孔石菇碱性磷酸酶的活性伍点来说突出在酶大分子的表面,EDTA容易与之发生络合作用,从而快速对小牛肠AKP产生抑制作用。

In aqueous solution no binding observed in crystal structure. In cyclohexane, one amylamine molecule is observed in the active site of trypsin and more sulfates are also found on enzyme surface, which are further proofs that electrostatic interaction are stronger in organic solvent.

在水溶液中,晶体结构难以观察到戊胺与牛胰酶的结合,在环己烷中,一个戊胺分子结合在胰酶的活性中心,同样酶表面观察到了更多的硫酸根,这些都进一步验证了有机溶剂中静电相互作用加强。

The method takes microcrystalline cellulose as affinity column chromatographic medium of Beta-glucosaccharase; under low temperature and anacid condition, the sample of Beta-glucosaccharase is loaded and specifically adsorbed to the microcrystalline column, and then the concentration of NaCl is increased to elute the enzyme, so as to achieve high-efficient and economic separation and purification.

本方法以微晶纤维素作为β-葡萄糖苷酶的亲和柱层析介质,在低温和微酸性条件下,将β-葡萄糖苷酶上样并特异性吸附于微晶纤维素柱,然后再通过增加NaCl浓度的方法将该酶洗脱,从而达到其高效而经济分离纯化的目的。

AF297709 , which has specific interaction with the promoter region of hTERT, was first screened out of the HeLa cells cDNA library with the method of yeast one-hybrid assary in 2000.It was found that TRAP has positive activation effect on the transcription starting of hTERT.

人端粒酶催化亚单位hTERT组分的水平决定了细胞中端粒酶的活性;hTERT表达是端粒酶激活的关键步骤,一些转录因子、肿瘤抑制因子、细胞周期抑制因子、细胞寿命决定因子、激素受体和病毒蛋白参与了hTERT表达调控。

Two area of autolytic enzyme system appeared in SDS-PAGE electrophoresis map,it was suggested that at least two kinds of enzymes with different mole...

在温度与自溶酶活性的研究中发现,分子量较小自溶酶的耐热性明显优于分子量较大的自溶酶。

Autolytic speed of lactic acid bacteria which is determined by the autolytic activity not only affect age velocity of cheese,but also influence the bacteria count of high density culture starter.

通过聚丙烯酰胺凝胶-细胞电泳法对乳酸菌杆LB-3细胞培养液中自溶酶的活性、细胞死亡与自溶酶的关系及温度对自溶酶活性的影响进行了研究。

Metal ions of Mn2+ stimulate the ChoA activity and Ca2+ significantly stimulate the ChoB activity, while Cu2+, Fe3+, and Ag+ inhibited them.

酶A的活性能够被Mn~(2+)激活,酶B的活性则能够被Ca~(2+)激活,Cu~(2+),Fe~(3+)和Ag~+则对两种酶的活性都有抑制作用。

Taking all the results mentioned above into consideration it is reasonable to conclude that the β-turn in the second hairpin of chymosin play a crucial role in determining the stability of prochymosin and pseudochymosin.

根据以上一系列结果,有理由认为凝乳酶第二个β-发夹结构的β-转角在决定凝乳酶原和假凝乳酶的稳定性中起至关重要的作用。

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