酶的

The optimal pH of xylanases was calculated based on the model and the results was shown as below: there were only two xylanses with the deviation between the calculated pHs the optimum pHs larger than 0.6, while there 6 xylanases with the deviation less than 0.1 the mean absolute percent error was 5.91%, the mean absolute error were 0.26 pH unit, respectively. It was superior when compared with the reported stepwise regression model based on dipeptide composition.

利用该方程，计算各木聚糖酶的pH值，结果表明，仅有2个木聚糖酶的最适合pH计算值与实测值相差大于0.6，而有6个木聚糖酶最适pH计算值与测定值相差小于0.1，平均绝对百分比误差为5.91%，平均绝对误差为0.26个pH单位，所得结果优于用二肽进行逐步回归所得模型的结果。

The results showed that the best carbon and nitrogen sources for xylanase production were lactose combined with beef extract.

结果表明，里氏木霉产两种酶的最佳碳源和氮源各异，其中木霉产木聚糖酶的最佳碳源为乳糖，氮源为牛肉膏；而产β-葡聚糖酶的最佳碳源为麸皮，氮源为硫酸铵。

The xylanase displayed about 50% retained acitivity after incubating at 80℃ for 30 min.

在pH 2.4～5.4范围内，酶的相对活性都在75%以上，重组酶在80℃保温30 min还有近50%的残余酶活。

As hollow fiber membranes thickness are different, yet urea clearance rate in simulation solution is relatively high, which can reach more than 64% and little difference among them; the BSA have a relatively high rejection, which can be achieved over 98%. There is a certain difference in the Lysozyme clearance rate, the smaller thickness, the higher the rate of removal, and the removal rate of 10μm thickness of the membrane is 29%. The clearance rates of urea were very high using different fiber diameter dialyzers, at around 80%. Urea clearance ratecan reach to the highest 88.6% using 0.26mm fiber diameter dialyzer. The clearance rates of Lysozyme vary greatly, the smaller the diameter, the higher clearance rate, and the highest removal rate of lysozyme is 62.79% using 0.26mm fiber diameter dialyzer. However, there is little impact on the rejection of BSA, which can reach 98%.

不同壁厚中空纤维膜透析器对模拟液中尿素的清除率均可达到64％以上，且差异不大，对牛血清白蛋白的截留率均可达到98％以上，对溶菌酶的清除率有一定的差异，壁厚越小清除率越高，壁厚为10μm的膜对溶菌酶的清除率为29％；不同纤维内径透析器对尿素的清除率均在80％左右，纤维内径为0.26mm的透析器对尿素的清除率最高，达到88.6％，对溶菌酶的清除率差别很大，内径越小，清除率越高，内径为0.26mm的透析器对溶菌酶的清除率达到62.8％，对牛血清白蛋白的截留影响很小，截留率都能达到98％以上。

Huang-hua' pear fruit was a typical climacteric fruit after harvest, and the fruit senescence-related factors, such as LOX activity, 02" content, AOS activity, ACC synthase activity, ACC content, ACC oxidase activity and ethylene production, changed with peaks at 20 "C. The activities of LOX, AOS, ACC synthase, ACC oxidase and the accumulation of O2 ", ACC content and ethylene synthesis were inhibited when stored at 1癈. And the severity of flesh floss and fruit decay was lightened. Low temperature combined with polyethylene film bag packing (PEF1 and PEF2) could further decrease the rate of weight loss, flesh floss, and fruit decay and keep preferable freshness. When PEF1 and PEF2 packing fruits were transferred to shelf life at 20 "C after storage at 1癈. The activities of LOX, AOS, ACC synthase, ACC oxidase could be suppressed and the accumulation of 02 , ACC content and ethylene production were slowed down to a certain extent, especially during the shelf life after 60 d storage. PEF1 and PEF2 packing fruit could maintain higher firmness as well, and the effects became more distinct along with the storage period, there existed no significant difference between PEF1 and PEF2 treatments.

试验结果表明，在20℃下，LOX活性、O2-水平、AOS活性、ACC合成酶活性、ACC含量、ACC氧化酶活性及乙烯的生成，均呈峰形变化；1℃处理可抑制LOX、AOS、ACC合成酶、ACC氧化酶的活性和O2-、ACC的积累，抑制贮藏果实的乙烯合成，减轻果肉发绵和果实腐烂。1℃下贮藏的果实，若结合不同聚乙烯薄膜袋(PEF1和PEF2)小包装，可以进一步降低果肉发绵率和果实腐烂率，保持果实新鲜度；当果实经过一段时间贮藏后，转移到20℃下，在货架期间，PEF1和PEF2对果实成熟衰老各个相关因子均有不同程度的抑制效应，主要表现在贮藏60d后的货架期间，同时维持较高的果实硬度，对硬度的影响随着贮藏时间的延长愈加明显，PEF1和PEF2处理效果之间无显著差异。

The genes of hammerhead ribozymes targeting against two and three sites on negative strand of potato spindle tuber viroid were designed, synthesized and cloned according to the action manner of hammerhead ribozyme.

根据锤头型核酶的作用模式，设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒负链RNA不同区域位点的双价和三价锤头型核酶基因。通过体外转录，将PSTVd负链RNA分别与双价和三价核酶混合，37℃温育 2h。

There were a large numberof ppts located in the pollen wall during pollendevelopment (Plate II-6), suggesting that ATPaseis necessary for the construction of pollen wall.

在真核细胞中，除了线粒体和叶绿体ATP酶的功能是合成ATP外，其余部位ATP酶是水解ATP以获取能量的代谢酶(Pederson和Carafoli1987)，在生物体细胞内广泛存在。

Within pressure treatments at 40MPa to 80MPa, the activities of plasmin and lipase were increasing.

研究结果表明，在40MPa-80MPa处理条件下，乳中纤溶酶和脂肪酶的活性都有所提高，但施加压力对酶活性的提高影响不显著(P＞0.05)。

Peroxidase in the spring leaf of orange cv.Jincheng was extracted. The optimum pH, optimum temperature, Km, Vmax and common inhibitors for POD were analyzed.

以锦橙成熟春梢叶片提取过氧化物酶，分别测定了该酶的最适pH、最适温度及酶促动力学参数Km值和Vmax值，比较分析了常见几种POD抑制剂的作用程度。

Fyn null mice display reduced adiposity associated with increased fatty acid oxidation, energy expenditure, and activation of the AMP-dependent protein kinase in skeletal muscle and adipose tissue.

这种新方法聚焦于一种间接控制&能量主开关&活动性的酶，这种酶叫Fyn激酶，如果给实验室老鼠注射抑制这种酶的化学物质，它们几乎马上开始燃烧更多脂肪。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。