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After 10 days, 20 days and 30 days for grass carp (Ctenpharyngoden idellus Cuvier et Valencinnes) immunized by being injected Aeromonas hydrophilia, the ANAE (acid α-naphthyl acetate esterase) positive cells ratioes increased, and the same time it was found that the ACP activity increased up too, it showed that the fish bodies were being at immune responding situation and their immnue activities enhanced.

通过对草鱼免疫器官的六种同工酶的分析发现：LDH在血浆、血液细胞、脾脏和胸腺中都表达为5条区带，前肾还额外显示出X酶带，血浆只有3条酶带；ADH除了胸腺为一条带以外，其它的均为3条酶带；MDH和3-GPDH在这些组织中均表达为3条酶带；EST在前肾中为7条酶带，其余的都为6条酶带；POX只在前肾和脾脏中表达为2条酶带。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数，结果表明：①酶-底物二元、三元复合物的失活速度明显慢于游离酶，说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用；②在脲作用下，酶的失活和构象变化均为单指数项过程，而且酶的活力丧失和三级结构变化是同时发生的，说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程；③在盐酸胍作用下，酶的构象变化为两相过程，而失活则是单指数项过程，酶分子构象变化的快相速度常数与失活速度常数基本一致，因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

But pancreatin is a mixtrure of various enzymes, each enzyme has a low purity and many enzymes have similar molecule structure and physicochemical properties, when extracting one enzyme, it is easy to abandon the otherone as impurity. This is why there are few technoloies of stimutaneous extraction of more than three enzyme from pancreatin and all the technoloies have shortages.

但是由于胰酶为多种酶的混合物，每种酶的纯度很低，许多酶又具有相似的分子结构和理化性质，因此往往在提取一种酶的同时，其它酶被当作杂质处理掉了，这就导致了胰酶中三种以上酶联产的工艺极为少见，而且已有工艺都有各自的不足。

Microphylla plantations are obvious, such effects and the contents of soil organic matter, N, P and K are gradually increased with the growth of C. microphylla plantations. Moreover, the activities of phosphomonoesterase, saccharase, protease, urease, dehydrogenase, polyphenol oxidase and nitrate reductase and the contents of edaphon C, N and P are significantly increased at soil depth of 0~30 cm, especially at soil depth of 0~10 cm.

随着小叶锦鸡儿固沙群落发育时间的增长，其综合防风性能提高，土壤有机质、N，P，K含量和生物活性逐渐得到改善。0~30 cm土层中磷酸单酯酶、蛋白酶、脲酶、蔗糖酶、硝酸还原酶、多酚氧化酶、脱氢酶的活性和土壤微生物生物量C，N和P含量均大幅度提高，其中0~10 cm土层增幅最大。

Similar to the histological changes, the shape of the enzym lesion focus was also global, it contained a center showed no enzym activities and a low enzym activity peripheral zone of 5～7 cell layers well-demarcated from the normal activity tissues.

结果：1。射频消融能明显破坏心肌组织多种代谢酶的活性，使乳酸脱氢酶、琥珀酸脱氢酶、细胞色素氧化酶和钙ATP酶活性丧失或减低，而且各酶的损伤范围相同。

749Mg/ml and 0.778u/ml, respectively. 5. Study on the viscosity changes of reaction solution and analysis of enzymatic hydrolysis products by TLC, HPLC showed that ChiB was an endo-type cleavage enzyme. It decreased viscosity of reaction solution, hydrolyzed β-(1, 4) glucosidic bond of chitosan, acted on GlcN-GlcN, GlcN-GlcNAc and/or GlcNAc-GlcN glucosidic bond. It did not act on GlcNAc-GlcNAc glucosidic bond. Hydrolysis products of chitosan were composed of chitotriose, chitotetraose and chitopentose.

通过测定酶解液体系粘度和对酶解产物的TLC、HPLC分析，在国内首次深入地研究并报道了内切、外切壳聚糖酶的酶解作用模式，结果表明：酶ChiB是一种内切酶，以内切方式水解壳聚糖的β-（1，4）糖苷键，即作用于GlcN-GlcN、GlcN-GlcNAc和／或GlcNAc-GlcN糖苷键，不能作用于GlcNAc-GlcNAc糖苷键；酶解终产物以壳三糖、壳四糖、壳五糖为主。

The result of immobilization showed that hydrophile membranes were more advantageous than hydrophobe ones to act as immobilization carrier. Crosslinking organophosphorus hydrolase by glutaraldehyde on polyethersulfone membrane is a simple and practical immobilization method. An appropriate amount of bovine serum albumin and crosslinker is necessary to get a good result of immobilization. In the biodegradation of methyl-parathion through enzyme membrane reactor, the effect of immobilized enzyme amount, flow rate of peristaltic pump, pH of the feed, and the methyl-parathion concentration on biodegradation rate was studied. It was shown that the biodegradation rate increased with immobilized enzyme amount and substrate concentration. Biodegradation rate didn't increase when the folw rate of the peristaltic pump was larger than 7 ml/min.

然后，研究固定化酶降解甲基对硫磷，从不同材料的膜载体固定有机磷水解酶的比较中，发现亲水膜比疏水膜适合作固定化酶载体；在此基础上，将有机磷水解酶固定在聚醚砜微滤膜上，并制成酶膜生物反应器，用于降解甲基对硫磷；戊二醛化学交联法固定时，酶液与10%牛血清白蛋白溶液的比例在3：1~2：1，与交联剂的比例在7：2~7：4范围内，固定化效果较好，并发现牛血清白蛋白和膜载体有降低酶活损失的作用；将固定化的酶膜装于酶膜反应器降解甲基对硫磷，实验结果表明：反应器的降解速率与固定化酶量呈正比，也随底物浓度的增加而加快，当流速低时，降解速率随流速增大而加快，到7ml/min以上时，降解速率不随流速脑黾佣洌到獾淖畹团ǘ任？

HTERT is expressed in cancer cell lines despite promoter DNA methylation by preservation of unmethylated DNA and active chromatin around the transcription start site.

人端粒酶逆转录酶是一种核糖核蛋白酶，由端粒RNA和端粒酶蛋白组成的一种特殊逆转录酶，在激活端粒酶的过程中起限速作用，能以自身RNA为模板合成端粒序列以稳定端粒长度使细胞获得永生，也是人类端粒酶的活性中心，其表达是端粒酶活化的必须前题［2～4］。

This article made a systematic and deep research on the property of soybean polyphenol oxidase and soybean peroxidase,including the function of the activity of polyphenol oxidase,heat stability,pH value as well as the inhibiting effect of soybean lecithin and dimercaprol.

对大豆氧化还原酶系中的多酚氧化酶和过氧化物酶的酶学特性进行了较深入系统地研究，其中包括酶活力、酶热稳定性、pH值的影响，以及大豆卵磷脂、二巯基丙醇对酶活力的抑制作用。

The effect of the flight on the activities of energy metabolism related enzymes in the flight muscles of the Oriental armyworm moth, Mythimna separata females was studied. These enzymes measured were glyceraldehydes-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase, lactate dehydrogenase, and 3-hydroxyacyl-CoA dehydrogenase. In the first 5 min of the tethered-flight of 3 day-old moth, activities of all the enzymes related to carbohydrate and lipid metabulistu increased rapidly, enzymes related to lipid metabolism were completely activated, and the activities of HOAD were significantly strengthened. But in flight duration from 5th to 60th min, the activities of all energy metabolism related enzymes decreased, suggesting that the flight was going to be stabilized.

对3日龄粘虫雌蛾吊飞过程中4种相关酶3-羟酰辅酶A脱氢酶、3-磷酸甘油醛脱氢酶、3-磷酸甘油脱氢酶和乳酸脱氢酶的研究结果表明，在室内条件下，粘虫在吊飞过程中其能量代谢有以下特点：在吊飞的初始5min，所有与糖代谢和脂肪代谢相关的酶活性都快速升高，这段时期脂肪代谢的酶活性也完全被活化，HOAD活性明显增强；但在随后的5~60min持续吊飞期间与能量代谢有关的酶活性都有所下降，表明此时飞行活性趋于平稳。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。