酶的

Erysimi CarE activity both of resistant populations and of susceptive population were trend to normal school, and the resistant L. erysimi populations have significant higher CarE activity than susceptive L. erysimi population. Thereinto, the activity level of CartE in Hongtang L. erysimi population is the highest among the four field populations, which is tally to the result of drug-fast test on each population.

对各地菜缢管蚜种群个体羧酸酯酶研究结果表明，无论是抗性种群还是敏感种群，其个体羧酸酯酶活性分布频率均趋于正态分布，即两头低，中间高；各抗性种群的羧酸酯酶的活性水平明显高于敏感种群，其中以洪塘菜缢管蚜种群的羧酸酯酶活性水平最高，这跟各种群的抗药性监测结果亦相吻合。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

Through the study on passivation condition of polyphenol oxidase of vegetable juice,orthogonal design experiment that dissociative pectase degradates the pectinase of vegetable juice and immobilized pectase dealing,draw the conclusion:(1) Polyphenol oxidase will be passivated by heating at 100℃ for 3 min,which prevents the concentrated juice from browning.

本试验通过对蔬菜汁多酚氧化酶的钝化条件的研究，以及游离果胶酶对蔬菜汁果胶降解的正交设计试验和固定化果胶酶处理，得出结论：(1)在100℃下加热3min可使多酚氧化酶钝化，达到防止褐变的要求。

Furthermore, treatment of the cultured tobacco cells with ABA also resulted in obvious increase in plasma membrane NADPH oxidase activity, and these enhanced effects of H2O2 production and NADPH oxidase activity induced by ABA were pronouncedly inhibited by two widely used mammalian neutrophil NADPH oxidase inhibitors, diphenylene iodonium and imidazole.

进一步研究发现，ABA能够显著升高质膜NADPH氧化酶的活性，而且ABA诱导H_(来源：A79BC论文网www.abclunwen.com)2O_2和NADPH氧化酶活性增加的效应能够被两个常用的NADPH氧化酶抑制剂碘二苯和咪唑显著抑制，这些结果说明，质膜NADPH氧化酶在悬浮细胞ABA诱导的H_2O_2快速产生中起重要作用。

The dissertation focuses on the breeding of Rhizopus chinensis 12# producing a fibrinolytic enzyme, optimization of solid-...

本论文对产纤溶酶的菌株根霉12#进行了诱变育种，优化了根霉12#产纤溶酶的固态发酵和液态发酵条件，建立了根霉纤溶酶制备分离纯化的方法并进行了优化，研究了根霉纤溶酶的性质。

For most viruses,there is aneed for antimicrobials that target unique viral molecular properties.Acycloviris one such drug.It is activated into ahuman herpesvirusDNA polymerase inhibitor exclusively by HHV kinases and,thus,does not suppress other viruses.Here,we show that ACV suppresses HIV-1in HHV-coinfected human tissues,but not in HHV-free tissue or cell cultures.However,addition of HHV-6-infected cells renders these cultures sensitive to anti-HIV ACV activity.We hypothesized that such HIV suppression requires ACV phosphorylation by HHV kinases.Indeed,an ACV monophosphorylated prodrug bypasses the HHV requirement for HIV suppression.Furthermore,phosphorylated ACV directly inhibits HIV-1reverse transcriptase,terminating DNA chain elongation,and can trap RT at the termination site.These data suggest that ACV anti-HIV-1activity may contribute to the response of HIV/HHV-coinfected patients to ACV treatment and could guide strategies for the development of new HIV-1RT inhibitors.

对大多数病毒而言，都需要有针对其分子特性的靶向杀毒剂阿昔洛韦就是这样一种靶向药物在人疱疹病毒酶的特定作用下，阿昔洛韦被激活成为人疱疹病毒DNA聚合酶抑制剂，因此不能再抑制其它的病毒我们的研究发现阿昔洛韦在共感染人疱疹病毒的组织中可以抑制HIV-1，但在无人疱疹病毒感染的组织或细胞中无此作用然而，加入人疱疹病毒-6感染的细胞却使得其对抗HIV的阿昔洛韦变得敏感我们推测这种抑制作用依赖于人疱疹病毒酶导致的阿昔洛韦磷酸化实际上，单磷酸化的阿昔洛韦前体药物无需人疱疹病毒的参与即可抑制HIV此外，磷酸化的阿昔洛韦能直接抑制HIV-1逆转录酶，将其阻止在终止位点，从而终止DNA链的延长这些结果提示阿昔洛韦的抗HIV-1活性决定了艾滋病病毒/人疱疹病毒共感染的患者对阿昔洛韦的治疗反应，也有助于开发新的HIV-1逆转录酶抑制剂

The last part of this article emphasis on the mechanism of cichory extract N2. Hepatic lipase, NO and xanthine oxidase are observed in this aspect, and gene expression of hepatic lipase is also detected.

本部分研究观察了菊苣提取物 N2 对影响脂质代谢的肝脂酶、尿酸代谢的关键酶黄嘌呤氧化酶、一氧化氮的活性和含量，还从分子生物学角度观察了肝脂酶的基因表达。

In this study, a strain of bacteria capable of producing Phenylalanine dehydrogenase was isolated and was identified as Achromobacter sp. The condition for the enzymatic fermentation, purification and characteristics of the enzyme were carried out in this work.

本研究从土壤中分离筛选到一株产苯丙氨酸脱氢酶的菌株X5-3，对其进行鉴定，并进一步研究了该菌株的产酶发酵条件、酶的初步纯化及该酶的性质。

Compared with the previous method, the improved route can provide directly a spheroid form of immobilized lipase particles of which the size can be controlled by adding non-ionic surfactants during the preparation process so as to increase the recovery rate of enzyme as well as decrease the external diffusional restriction which can diminish the enzyme activity. The improved method can also alter the hydrophobicity and microstructure of the immobilized enzyme matrix by changing the molar ratios of different alkoxyl silanes so as to enhance the enzyme activity by a"surface active effect"and eliminating the internal diffusional restriction.

与前人的制备方法相比，改进的方法可直接制得球形的固定化酶颗粒，而且颗粒的大小可以通过在制备过程中加入非离子型表面活性剂加以控制，从而可以提高固定化过程的酶活回收率和减小外扩散效应对酶的表观催化活力的影响；改进的方法还可以通过改变原料硅氧烷的种类及配比来改变最终制得的固定化载体的疏水性质及微观结构，从而通过对脂肪酶的&界面活化&作用和减小内扩散效应提高固定化脂肪酶的表观酯化活力。

The distribution, properties,structure and regulation of plant allantoinase were reviewed in detail.

文摘：全面综述植物酰脲降解代谢酶的研究现况，概述植物尿囊素酶的分布、性质、结构，以及调节控制，并简介植物尿囊酸酰胺水解酶和脲基乙醇酸酰胺水解酶的研究近况，同时指出待研究的方向。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。