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The protease secreted from cellcould be more stable by adding the Ca2+ to fermentation broth. The cold-adapted protease, stable at 15℃, could be secreted in large quantity by the strainof SYP-A3-2. When the culture temperature was changed from 15℃ to 20℃, the strain grewfaster while the enzyme yield was decreased.

SYP-A3-2菌株在15℃下能大量分泌低温蛋白酶,且能有效保持酶的稳定性,在20℃时能以较快速率生长,但是酶量很少且酶活力不高,可以设计温度控制方案进行分阶段培养以缩短发酵周期。

Suitable substrates were the key factors for xylanases production. XylanaseB was produced mainly when corn starch or beechwood xylan was used as the inducing substrate.

发酵底物是产生木聚糖酶的重要影响因素,在以玉米淀粉或山毛榉木聚糖为底物时,产生的木聚糖酶主要是木聚糖酶B,而以廉价的麦麸为底物则主要产生木聚糖酶G和B。

The highest enzyme producer alkalophilic Bacillus sp SHY8-1 among the inductive strains was mutagenized several times, by adding enzyme production repression aegents into screen medium, combined with natural seperation and pressure selection, finally a CMCase-hyperproductive mutant SHY8-1-25 (partitally resistant to catabolite) was obtained, with the yield of CMCase raised to 13-16u/ml, maximally reached 18-20u/ml, while its parent strain 0.654u/ml.

对诱导型产酶菌-嗜碱芽孢杆菌SHY8-1进行了多次诱变改良,采用添加产酶阻遏剂的平板作为筛子,结合反复的自然分离、压力选择,最终成功地获得了高产变异株SHY8-1-25(已部分解除分解代谢阻遏),产酶量由0.654u/ml提高到13-16u/ml,最高达到18-20u/ml,讨论了诱变机理策略,考察了碳、氮源等对产酶的影响,对培养基和摇瓶培养条件进行了优化。

Methods: We divide the rats into 5 groups in random, with Morris method, detect the change of some active oxygen radicals such as super-oxide demitasse, Nitric Oxide Synthase, malondialdehyde etc. Also, we detect the change of cholinesterase, Monoamine Oxidize; using HE,PAS staining, immuno-histochemical technique, detect hippocampus CA1 cerebral pathology slices optical density value of NOS, CHE and lipofuscin of neural cell; detecting apoptosis rate and the expression of P53 , which are neuronal apoptosis related genes.

大鼠随机分为5组,采用"Morris水迷宫"法观察脑缺血性模型大鼠学习记忆功能,检测超氧化物歧化酶、一氧化氮合酶、丙二醛等自由基代谢异常的改变,检测胆碱酯酶、单胺氧化酶等老化相关酶的变化、脑组织神经细胞免疫组织化学技术、检查海马CA1区脑组织的病理切片,检测NOS、CHE光密度值;流式细胞技术:检测细胞凋亡率,及促细胞凋亡基因、P_(53)蛋白表达;病理切片采用HE染色、PAS染色及免疫组化法检查,检测对大鼠海马神经元细胞一般病理变化及脑细胞内脂褐含量。

Coffee plants are tansformed with vectors containing ACC synthase and/or with ACC oxidate DNA sequences that code on expression for the respective mRNA that is antisense to the mRNA for ACC synthase and/or ACC oxidase.

用含有编码表达相应mRNA的ACC合成酶和/或ACC氧化酶DNA序列的载体转化咖啡植物,所述mRNA与编码表达ACC合成酶和/或ACC氧化酶的mRNA反义。

The prosequence had no obvious effects on the characteristics of RCL, which was different from Rhizopus oryzae lipase.

与米根霉脂肪酶不同,前导序列对华根霉脂肪酶的主要酶学特性没有显著影响。

The purpose of this study is to reveal the change characteristics of biological properties of sandy soil and its development trend under the establishment of C. microphylla plantations in flowing sand dune. With semi-moving sand dune and natural community as controls, the activities of phosphomonoesterase, saccharase, protease, urease, dehydrogenase and the Contents of microbial biomass C, N, P of the soil in an age sequence (5-, 10-, 22-year) of C.

为探讨采用小叶锦鸡儿固定流沙后,土壤生物活性的变化特点和发展趋势,选取5、10、22年生小叶锦鸡儿人工固沙群落为对象,以半流动沙丘和天然群落为对照,研究了人工固沙群落发育过程中土壤磷酸单酯酶、蔗糖酶、蛋白酶、脲酶、脱氢酶的活性和微生物生物量C、N、P质量分数的变化特征。

Study on the optimal conditions of cellulase from termites The optimal pH when Cx from Reticulitermes flaviceps reacted with substrate sodium carboxymethylcellulose was between 5.0-7.4 and 5.0-6.2 when BG enzyme was reacted with substrate salicin, the relation between activities of enzymes and relevant pH values accorded with Quadratic models.

一、白蚁纤维素酶最适反应条件研究黄胸散白蚁 Reticulitermes flaviceps C_N酶与羧甲基纤维素钠(Carboxymethylcellulose sodium,CMC-Na)反应的最适pH值范围在5.0-7.4之间,BG酶与Salicin反应的最适pH值为5.0-6.2,2种酶的活性均与pH值符合二次项曲线模型。

The O-2 production rate, H2O2 content, superoxide dismutase, peroxidase, ascorbate peroxidase, catalase and polyphenol oxidase and MDA content of petal of two tree peony cultivars 'Luoyanghong' and 'Huhong' during florescence and flower senescence were detected by the methods of conuentional biochemistry. The relationships among MDA content and others index were analyzed by the simple correlation coefficient, stepwise regression and the path-coefficient.

测定"洛阳红"和"胡红"2个牡丹品种开花和衰老过程中花瓣膜脂过氧化产物丙二醛、超氧自由基O(上标–下标 2、过氧化氢(H2O2)的含量及超氧化物歧化酶、过氧化物酶、抗坏血酸过氧化物酶、过氧化氢酶、多酚氧化酶的活性,并采用简单相关分析、逐步回归分析和通径分析等方法研究MDA含量与其他指标的关系。

The results show that the plant communities naturally colonizing on the wastelands markedly increased enzyme activities (urase, arylsulfatase, alkaline phosphatase and dehydrogenase) in the upper layer of the tailings (0~5 cm).

结果表明,自然定居在尾矿废弃地上的植物群落类型能够显著影响表层尾矿中(0~5cm)的脲酶、芳基硫酸酯酶、碱性磷酸酶和脱氢酶的活性。

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