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The cell growth and the releasing rate of urease from bacteria and actinomycete in the presence of different nBPT concentrations were examined.

研究了脲酶抑制剂nBPT对土壤中脲酶活性和细菌、放线菌生长及发酵产酶的影响。

The different factors involved in the process of the folding and activation of the microbial lipase are reviewed in this article,including lipase-specific foldase,lipase activation factor,prosequence,calcium ion and disulphide bond.

对微生物脂肪酶活性构像的形成和激活机制进行深入研究,有助于我们更好地了解脂肪酶的结构和功能,为脂肪酶基因的大量表达和体外定向进化奠定基础。本文主要综述了作为脂肪酶结构成分的多种因子在脂肪酶活性构像形成、激活及稳定中的作用。具体催化反应体

Therewas a significant characteristic of the vertical distribution of soil enzyme activity,the relationship analysis indicated that there was a significant positive relationshipbetween the catalase activity and OM,and total N,and effective P、K,and ex-changible Mg,there was a negative relationship between the catalase activity andBase Situatiou percentage,and Total Contents of Base;the activity of the polyphe-nol oxidase was positively related with OM,and negatively related with total N andexchangible Ca clearly;The urease activity decreased with CPR increasing,and thechange of the former two kinds of soil enzyme actirity was not significantly withCPR increasing,It is possible to be related with maned disturbance frequently.

随着代次增加,有机质和交换性Ca下降明显;土壤的CEC和交换性酸分别下降了8.8—16.1%和6.8—18.5%,因此,其养分总量亦逐代降低,其中有机质下降幅度最大;土壤酶的活性具有明显的垂直分布特征,相关分析表明,过氧化氢酶活性与土壤OM、全N、有效P、K和交换性Mg有极显著的正相关,而与盐基总量、盐基饱和度呈明显的负相关。多酚氧化酶活性与OM呈显著负相关,与全N、交换性Ca有显著相关。脲酶活性则有随连栽代次增加而有下降趋势,前两种酶在代次之间变化不明显,可能与频繁的土壤干扰有关。

U 2+, Co 2+ and Mn 2+ whose concentrations were 40, 40, 20μg·L -1 respectively could increase the AKP activity significantly in mysis, but higher con...

o2 +在 2 0μg·L-1时明显提高两种酶的活性。而Ni2 +只使两种酶活性降低。鉴于碱性磷酸酶和ATPase与生长发育直接相关,金属离子对对虾幼体生长的影响与对两种酶活性的影响基本一致,因此体内这两种酶活性的高低可作为判别环境因素是否适合对虾幼体生存的指标。

Results indicate that effect of a dip and pile method is better than that of paint and pile method and activities of protease JW-2 and effect of enzymatic unhairing are accelerated by sodium silicates. Further more, complete dehairing effect is obtained by 1% sodium silicate and 4% enzyme JW-2 and grain shows smooth and clear.

结果表明:浸酶堆置脱毛方式的效果优于涂酶堆置脱毛;硅酸钠对JW-2蛋白酶的活力及浸酶堆置酶脱毛的效果有一定的促进作用;当硅酸钠和酶的用量分别为1%和4%时,可产生完全脱毛效果,且脱毛后裸皮的粒面光滑、白净。

Times higher than that of initial strain. Polysaccharides, such as barley flour, dextrin and soluble starch, were better carbon sources than monosaccharides and disaccharides, such as glucose and maltose, for cell growth of B subtilis ZJF-1A5 and β-glucanase production. Yeast extract was the best nitrogen source, the following was soybean flour.

糊精、大麦粉等多糖有利于B.subtilisZJF-1A5 β-葡聚糖酶的产生,葡萄糖、蔗糖等易利用性碳源不利于菌体生物量的积累和β-葡聚糖酶的产生,β-葡聚糖酶受分解代谢产物阻遏调节。B.subtilis ZJF-1A5 β-1,3-1,4-葡聚糖酶的产生与菌体生物量和菌体生长状态密切相关,提高发酵产酶水平的关键是提高菌体生物量和解除葡萄糖效应。

I developed bee protein feed high in protein, fats, minerals, fat soluble vitamins, the proportion of suitable water-soluble vitamins, feed ratio of computational science, in line with bee nutritional requirements; feed is also appropriate to add grape oxidase, amylase, invertase, protein hydrolytic enzymes, fat bees enzymes necessary enzymes, raising bees on nutrient absorption rate, while reducing excretion, so that more fully reflects the use of nutrition.

我开发蜂蛋白饲料高蛋白质,脂肪,矿物质,脂肪和水的比例适当的水溶性维生素,饲料比例的计算科学,与蜜蜂营养要求,饲料也应加重葡萄氧化酶,淀粉酶脂溶性维生素,蔗糖酶,蛋白水解酶,脂肪酶的必要蜜蜂酶,提高对养分的吸收率蜜蜂,同时减少排泄,因此更充分地反映了营养的使用。

Characterization of senescence-associated endopeptidase iso -zymes in wheat leaves The characterization of senescence-associated endopeptidase isozymes in wheat leaves during dark-induced senescence was performed.

而采用将明胶为底物的SDS-PAGE方法只能检测到一种新内肽酶同工酶的出现,因此衰老期间表达的内肽酶同工酶大都是对SDS敏感的或多亚基的蛋白水解酶。

Compared the activities of 5 sorts of lipases from different source in synthesis of fatty acid esters and hydrolysis of olive oil, 3 sorts of lipases are better. Compared the stability and the activities of 3 sorts of Upases in different media, organic solvents and CAL lipase are best.

通过对不同来源的5种脂肪酶分解橄榄油和合成脂肪酸酯的比较,选出了合成脂肪酸酯活力较高的3种脂肪酶,通过比较不同介质中这3种脂肪酶的合成活力后,得出这三种酶在有机相中活力最高,从操作稳定性角度出发,选出催化酯合成效果最好的脂肪酶CAL。

Six senescence-associated endopeptidase isoenzymes were identified by using natural gradient-polyacrylamide gel electrophoresis co-polymerized with gelatin in the gel, five of which (EP1、EP2, EP4、EP5 and EP6) were only detected in senescing leaves.

另外,采用不同的内肽酶同工酶电泳方法研究中均发现自然衰老和暗诱导衰老过程中的内肽酶同工酶谱变化基本相似,因此暗诱导衰老可以作为一种研究叶片自然衰老过程中出现的蛋白水解酶的特性的模式。

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