酶的

They were reversible un-competitive inhibitors, and their inhibition constants of free enzymeK(subscript I were 254.00 and 136.78 mmolL^(-1), respectively. Urea was reversible mixed-type inhibitor, and its inhibition constants of free enzymeK(subscript I and enzyme-substrate complexK(subscript IS were determined to be 379.02 and 1182.00 mmolL^(-1), respectively.

Na2SO3和H2O2对酶的抑制均表现为可逆反竞争性类型，它们对游离酶的抑制常数K(下标 I分别为254.00和136.78 mmolL^(-1)；尿素对酶的抑制表现为可逆的混合型抑制，它对游离酶的K和对酶－底物复合物的抑制常数K(下标 IS分别为379.02和1182.00 mmolL^(-1)。

Isolation, purification and characteristics of D-hydantoinase were carried out. The experiment results showed that the optimal pH and temperature of pure D-hydantoinase were pH 8. 5 and 35℃, respectively. D-hydantoinase activity could be increased significantly by Mn〓 and Fe〓. Substrate specificity of D-hydantoinase on various hydantoin derivatives showed the optimal substrate of D-hydantoinase was 5-phenylhydantoin, but hydantoin, 5-methylthioehylhydantoin, 5-hydroxyphenylhydantoin, 5-methylhydantoin, 5-benzylhydantoin could also be hydrolyzed by D-hydantoinase.

本文还进行了海因酶的分离纯化及性质研究，发现酶的最适反应温度为35℃，最适pH为8.5，金属离子Mn〓和Fe〓能显著提高D-海因酶活力。D-海因酶的特异性实验表明，D-海因酶的最适底物为D，L-苯海因，其次为海因，D，L-对羟基苯海因和D，L-甲硫乙基海因，对D，L-甲基海因和D，L-苯甲基海因也有一定的作用。

The results indicate seawater concentration has no effect on absorbency, pH, relative density, dry matter and water contents in Aloe vera leaves. While, seawater stress causes increased MDA and membrane penetration, decreased phytochroms, prolines and NO_3~--N. It is beneficial to plant growth when irrigation was done with 10% seawater, because of higher ratio of K~+/Na~+ and lower ratio of Na~+/Ca~(2+). In addition, the polysaccharide contents in Aloe vera leaves increase twice and triplication under 25% and 50% seawater irrigation respectively, the barbaloin contents increase over triplication in var. 75% seawater and over twice in var. 50% seawater and var. 100% seawater. However, when seawater concentration exceeds 25% the growth of plant was inhibited, biomass decreased, flowering time was deferred, Ca~(2+) content decreased caused by Na~+ accumulation in leaves.

结果表明：在海水胁迫下，库拉索芦荟全叶汁的吸光度、酸度、相对密度、总固形物和水分含量总体上受海水浓度的影响不大；但芦荟叶的丙二醛含量和质膜透性增加，叶绿体色素、脯氨酸和硝态氮含量减少；在10％海水浇灌下，芦荟叶细胞具有较高的K~+／Na~+比和较低的Na~+／Ca~(2+)比，对库拉索芦荟的生长有一定的促进作用；在25％海水和50％海水浇灌下，芦荟多糖含量提高了2-3倍；在75％海水浇灌下芦荟甙含量增加了3倍多，在50％海水和100％海水灌溉下，其含量也增加了2倍多；当海水浇灌浓度超过25％后，库拉索芦荟的生长明显受到抑制，生物量降低，抽苔期推迟，同时，海水浓度的增加也导致芦荟叶中累积大量Na~+，从而使得芦荟叶中Ca~(2+)含量降低，但Mg~(2+)并没有受到海水浓度的影响；用浓度低于75％的海水浇灌库拉索芦荟可以降低剖面深度在0-100cm之间的土壤盐度；其抗氧化保护酶中超氧化歧化酶的酶活力相对保持稳定，过氧化物酶的酶活力在10％海水浇灌下明显提高，随后则下降，而过氧化氢酶的酶活力则随海水浓度的升高而下降。

Spraying with chitosan of different concentration significantly increased the content of chlorophyll ,anthoxanthin ,chlorogentic acid, polyphenal ,soluble protein and total soluble sugar of Broccoli flowers. 40 mg L-l chitosan showed the most remarkable effet,compared to control, the content of chlorophyll ,anthoxanthin ,chlorogentic acid, total soluble sugar and soluble protein of treated Broccoli flowers increased by 64.4%,282.5%,35.6%,16.2% 22.7% respectively.PAL and PPO are key enzymes in synthesize of phenolics.No relation was found between phenolics synthesize and PAL activity in Broccoli flowers,but it showed positive relation between phenolics synthesize and PPO activity.

研究表明，本研究中，不同浓度几丁聚糖喷施对青花菜花球的叶绿素、类黄酮、绿原酸、多酚总量、可溶性蛋白及可溶性总糖的含浙江大学硕卜学位论文(2004)量有显著增加，其中以浓度为40mgL一'影响最为显著，花球中的叶绿素、类黄酮、绿原酸、可溶性总糖、可溶性蛋白分别较对照提高64.4%、282.5%、35.6%、 16.2%、22.7%，苯丙氨酸解氨酶、多酚氧化酶是酚类物质合成中关键酶，花球内酚类物质合成与苯丙氨酸解氨酶的活性没有一定的相关性，而与多酚氧化酶的活性呈正相关。

Methods Glutamate decarboxylase was embedded-immobilized with sodium alginate and gelatin as the blending supports. Factors which affected the activity of IGAD were studied and optimum conditions were investigated. Afterwards, IGAD was applied for GABA transformation.

以海藻酸钠与明胶为协同作用载体包埋固定化酶，考察影响固定化酶活性的因素，获得固定化酶的优化条件，并将酶应用于制备GABA。

In addition, isozymes changes of SOD and POD were observed in groups treated with laser after seeds being soaked for 24 h and when embryonic buds emerged, while no change was observed in the group treated at the one-leaf stage.

SOD、POD同工酶的酶谱在浸种24小时和胚芽露头期经激光处理后改变，在一叶期处理后没有改变；CAT同工酶谱没有变化；在一叶期处理的三种同工酶谱都没有改变。

In this review, the molecular structure, substrate specificity, catalytic mechanism and other physicochemical parameters of laccase are summarized. The role of laccase in plant cell wall formation and pathogen virulence are discussed.

本文叙述漆酶的分子结构、底物特异性及其物理化学特性，并讨论漆酶的酶促反应机理和生物学功能，包括植物漆酶参与细胞壁的形成以及漆酶与病原菌毒力的关系。

In this review, the molecular structure, substrate specificity, catalytic mechanism and other physicochemical parameters of laccase are summarized. The role of laccase in plant cell wall formation and pathogen virulence are discussed.

本文敍述漆酶的分子结构、底物特异性及其物理化学特性，并讨论漆酶的酶促反应机理和生物学功能，包括植物漆酶参与细胞壁的形成以及漆酶与病原菌毒力的关系。

The changed plocess of MDA, SOD, POD and CAT in the body of persimmon, which was harmed by Japanese wax scale, was researched on the scales of lightly and moderate and heavily damaged degrees in order to show the relations between the protected enzymes in persimmon and resistance-insect.

柿树受日本龟蜡蚧危害后，分别以轻微受害、中等受害、严重受害三个等级研究了柿树体内丙二醛及超氧化物蚊化酶、过氧化物酶、过氧化氢酶的动态变化过程，旨在揭示树体保护酶与树体抗虫作用的关系。

The total activity of EPs increased at first and then decreased during both senescences. The changes of endopeptidase isozymes in wheat leaf senescence were studied by using natural gradient-polyacrylamide gel electrophoresis co-polymerized with hemoglobin or gelatin in the gel.

主要研究内容如下： 1、小麦叶片衰老过程中内肽酶的变化蛋白质的降解需要蛋白水解酶，包括内肽酶和外肽酶的参与，叶衰老过程中发挥主要作用的是内肽酶。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。