酶的

To study the function of chymase,the protease inhibitors including Lisinopril,Aprotinin and EDTA,and radioimmunuoassy were used to measure the activities of angiotensin converting enzyme and chymase in the heart tissues of transgenic mice harboring the human heart chymase gene and the controls.

为研究糜酶的功能，本文利用蛋白酶抑制剂Lisinopril、Aprotinin及EDTA结合放射免疫分析的方法同时测定了糜酶转基因小鼠心脏组织中血管紧张素转换酶（angiotensin converting enzyme，ACE）及糜酶样活性。

By n-butanol extraction, ammonium sulfate fractionation and sodium chloride dialyzation, the partially purified enzyme was gained by means of gel filtration on sephadex G-150 column, and some properties of the enzyme were determined.

方法］以DFRKN-1为提酶材料，经正丁醇提取，硫酸按分级沉淀分离，透析获得酶的粗提取液，用SephadexG-150凝胶过滤柱层析纯化，获得纯化碱性磷酸酶，并测定纯化碱性磷酸酶的理化性质。

In this review, the biosynthetic pathway of ginsenosides was introduced and the research progress on enzymes related to ginsenoside biosynthesis , such as 3-hydroxy-3-methyl-glutaryl-CoA reductase, geranyl pyrophosphate synthase, farnesyl pyrophosphate synthase, was summarized in detail.

本文综述了人参皂苷的生物合成途径，并介绍了3-羟基-3-甲基戊二酸CoA还原酶、牻牛儿基二磷酸合成酶、法尼基二磷酸合成酶等人参皂苷合成途径相关酶的研究进展。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

Phytase mainly comes from plant and organism, phytase of plant belongs to 6-phytase and it s optimum pH ranges from 5.0 to 7. 5. Plant phytase can not function effectively in monogastric animals and it is not rich in plant. So research direction about phytase is turned to Aspergillus niger which is rich in acid phytase.

植酸酶主要来自于植物和微生物，来源于植物的植酸酶均属于6—植酸酶，最适pH范围在5.0—7.5，不适合在单胃动物中起作用的，而且在植物中含量极低，因此植酸酶的研究方向转向最适pH值为酸性、酶含量较高的丝状真菌。

A Lower concentrationof sodium sulfide associatedwith the complex enzyme was studied for reducing the amount of sulfide in the paint unhairing of yak hide .

采用碱酶(一种碱性蛋白酶、碱性脂肪酶和糖化酶的复合酶)结合脱毛法，目的是降低牦牛皮脱毛工序中硫化钠的用量。

LOX activity in barley was also studied during germination of barley and kilning, including the pattern of steeping, steeping additives and kilning processes.

本文研究了大麦中脂肪酸氧化酶的基本酶学性质，结果表明：大麦脂肪酸氧化酶的最适pH为6.0，最适作用温度范围在30~40℃，在70℃时酶活损失较大。

The preparation of immobilized enzyme: The inulinase, used in the fructose production from Jerusalem artichokes, and the glucoamylase, used in the glucose production from dextrine were chosen as samples. The comparative tests were carried out on specially designed setups for the immobilization of enzymes, and a synthetic evaluation was made of the quality of the immobilized enzymes.

固定化酶制备方法的选择：选定了降解洋姜液生产果糖工艺的菊糖酶及降解糊精生产葡萄糖工艺的糖化酶分别进行了不同方法的酶的固定化；设置了相应的实验装置，进行对比实验，研究了各因素对酶的固定化的影响；并对所制备的固定化酶的效果进行了综合评价。

In this thesis, the submerged fermentation and solid-state fermentation of Coriolus versicolor laccase, the enzymatic characterization, and the application of laccase in indigo and gold orange Ⅱ decolorization was investigated. The major results were as follows:The laccase production by Coriolus versicolor was performed in submerged fermentation, using corn husk as carbon source and carbamide, ammonium sulfate as nitrogen source.

本文对Coriolus versicolor漆酶的液体发酵和固态发酵进行了研究，并且对Coriolusversicolor漆酶的酶学性质及其在靛蓝、金橙Ⅱ脱色降解方面的应用进行了探讨，其研究成果如下：对Coriolus versicolor漆酶的液体发酵工艺参数进行了优化，结果表明，以玉米皮为碳源，尿素和硫酸铵为氮源，利用Coriolus versicolor液体发酵产漆酶时，C／N以20为宜。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。