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Through four steps of column chromatography and electrophoresis purification, one kind of nuclease was obtained.

样品依次通过四步柱层析,PAGE-DNA功能胶纯化得到电泳纯的双胸蚓核酸酶样蛋白质纯品,命名为DNase E。2。

And cell differentiation was assayed by measuring the cellular alkaline phosphatase activity with dexamethasone as positive control. The influence of ethanol and dimethyl sulfoxide on cell proliferation and differentiation assay was also investigated.

成骨样细胞的分化采用测定细胞内碱性磷酸酶活性的方法,地塞米松为阳性对照药,同时考察了溶剂乙醇和二甲基亚砜对细胞增殖和分化的影响。

The results suggested that the chymase could convert Ang I to Angin vivo,and the radioimmunoassay method could detect the activities of ACE and chymase simultaneously with high sensitivity and credibility.

表明糜酶转基因小鼠心脏中可表达糜酶且糜酶能催化Ang II 的生成,而蛋白酶抑制剂结合放免分析是一种快速、灵敏的检测ACE及糜酶样活性的方法。

To study the function of chymase,the protease inhibitors including Lisinopril,Aprotinin and EDTA,and radioimmunuoassy were used to measure the activities of angiotensin converting enzyme and chymase in the heart tissues of transgenic mice harboring the human heart chymase gene and the controls.

为研究糜酶的功能,本文利用蛋白酶抑制剂Lisinopril、Aprotinin及EDTA结合放射免疫分析的方法同时测定了糜酶转基因小鼠心脏组织中血管紧张素转换酶(angiotensin converting enzyme,ACE)及糜酶样活性。

Based on the sources, molecular weights, pI, characters of chromatography and electrophoresis, substrate specificities of these enzymes, it can be concluded that many kinds of nuclease-like enzymes have been found in the tissues of Lumbricus Bimastus.

根据这些酶的来源、分子量、等电点、层析和电泳等行为以及底物特异性等,初步证明,我们新近发现了双胸蚓组织中存在着多种核酸酶样酶。

The Heterotrophic bacteria, nitrosomonas, epiphyte and rhizosphere in non-rhizosphere zones are more than the contrast in the sample with the inoculants and enzyme, contrary to the nitrobacter and denitrifying bacteria.

菌剂和酶制剂的加入使得非根际泥样异养细菌、真菌、放线菌和亚硝酸细菌比对照组稍高,硝酸细菌和反硝化细菌则较少;而根际泥样中加菌剂和酶制剂组微生物数量比空白组高出1—2个数量级,在投加菌和酶制剂后酶活性无论在根际还是非根际都得到提高,高于对照组约5—6个酶活单位。

Objective: To confirm the nucleolar localization of telomerase-regulation associated protein-human N-acetyltransferase-like protein and its associated functions.

目的:探讨端粒酶调节相关蛋白-乙酰转移酶样蛋白核仁定位的分子基础及相关功能。

The calf intestinal epithelial cells in vitro were selected to evaluate the trophic actions of insulin, IGF-J and peptic and tryptic hydrolysates of bovine colostrum. The cell proliferation, glucose absorption and specific activity of cell membrane enzymes were investigated.

为研究乳源性生长因子对动物胃肠道生长发育的作用,本试验以离体犊牛小肠上皮细胞为试验对象,研究了胰岛素、胰岛素样生长因子Ⅰ和酶解牛初乳对细胞增殖、葡萄糖吸收和细胞膜酶活性的影响。

Kelch-like ECH-associated protein-1 (Keap1) normally sequesters Nrf2 in the cytoplasm in association with the actin cytoskeleton, but upon oxidation of cysteine residues Nrf2 dissociates from Keap1, translocates to the nucleus and binds to ARE sequences leading to transcriptional activation of antioxidant and phase Ⅱ detoxifying genes.

在正常情况下,Kelch样环氧氯丙烷相关蛋白-1(Kelch-like ECH-associated protein-1, Keap1)与Nrf2耦联,并与肌动蛋白细胞骨架结合被锚定于胞浆,但是在半胱氨酸残基发生氧化的情况下,Nrf2和Keap1解耦联,进入细胞核并与ARE结合,从而激活多种抗氧化基因和Ⅱ相解毒酶基因的转录。

The effects of gastrodine on the expression of glial fibrillary acidic protein, the transuding amount of lactic dehydrogenase, and the activity of nitric oxide synthase in cultured astrocytes of young rats with lesions caused by simulated cerebral ischemia and reperfusion, were determined in this experiment.

观察天麻素对脑缺血再灌注损伤星形胶质细胞胶原纤维酸性蛋白表达、乳酸脱氢酶漏出量以及一氧化氮合成酶活性的影响。结果:天麻素大、小剂量组GFAP纤维样改变减轻,强阳性反应细胞数减少;LDH漏出量下降;NOS活性减弱。

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