酶促作用

Aqueous extract of cotton has allelopathic effect on Raphanus sativus, Brassica campestris, Lactuca indica, Brassica campestris, Echinochloa crusgall, Setaria viridis and Abutilon theophrasti and its inhibition effect gradually strengthens with increasing concentration. Aqueous extracts from stems, leaves and roots of cotton inhibit or accelerate seed germination and seedling growth, and activity of enzyme of seedling root of cotton. While aqueous extracts from stems and leaves of cotton are 0.02 gmL^(-1), root length, shoot length, lateral root number and dry weight of cotton respectively increase by 10.3%, 3.1%, 22.9% and 2.4% over the control. The above mentioned indexes are lower than the control by 34.4%, 39.2%, 40.6% and 19.0% respectively as the aqueous extracts are 0.1 gmL^(-1). At the same time, root activity and SOD activity decrease by 38.2% and 36.7%, while POD activity and MDA content increase by 52.3% and 36.5%. Growth indexes of plant receptors increase at low concentrations but decrease at high concentrations of aqueous extracts. The allelopathic effect of aqueous extract of stems and leaves is stronger than that of root.

结果表明：棉花植株水浸提液对萝卜、白菜、莴苣、油菜、稗草、狗尾草、苘麻均具有化感效应，随着作用浓度增加，抑制效应逐渐加强；棉花茎叶和根系水浸提液对棉花种子萌发、幼苗生长、幼苗根系活力、根系酶活性有抑制或促进作用，当茎叶浸提液浓度为0.02 gmL^(-1)时，棉花根长、苗高、侧根数、干重分别比对照增加10.3%、3.1%、22.9%和2.4%，当浓度为0.1 gmL^(-1)时，上述指标与对照相比分别降低34.4%、39.2%、40.6%和19.0%，而根系活力、SOD活性比对照降低38.2%、36.7%，POD活性、MDA含量较对照增加52.3%和36.5%，不同浓度棉花植株水浸提液对棉花生长呈现"低促高抑"的规律，且茎叶浸提液作用效应大于根系浸提液。

Purpose To study the mechanism of effects of aldose reductase inhibitors on rat mesangial cells in vitro proliferation induced by PDGF-BB.

目的 探讨醛糖还原酶抑制剂影响血小板源性生长因子－BB促体外培养大鼠系膜细胞增殖作用的机制。

The effects of FPL andits three ingredients on growth of ovary,on micro-and ultra-structure andAlkaline phosphatase activity of endometrium,on structure andkeratinization rate of exfoliated cells in vaginal epithelium in mice,onmicrocirculation in rat and on uterine kinesis in rabbit were respectivelyobserved by the techniques of light and electron microscopes,histochemistry,microcirculation and organ cinetography.The results have confirmed themechanism of FPL for the sterility caused by ovary standstill and persistentcorpus luteum to be as follows:(1) a weak estrogen-like role which canprompt development of ovary,proliferation and secretion of endometrium andvaginal epithelium and cornification of exfoliated cells in vaginal epithelium;(2)enhancing AKP's activity of endometrium;(3)improving microcirculation;(4) exciting uterus.

分别采用光学显微镜和电子显微镜技术、组织化学技术、微循环技术和器官运动描记等现代医学实验技术，观察了促孕液对小白鼠的卵巢发育、子宫内膜的显微和超微结构和碱性磷酸酶活性、阴道上皮结构和脱落细胞的角化率、对大白鼠的微循环和对家兔的子宫运动的影响，结果证实了促孕液治疗卵巢静止、持久黄体的机理：(1)微弱的雌激素样作用，能刺激卵泡发育，促进子宫内膜和阴道上皮增生、分泌和阴道上皮脱落细胞角化；(2)提高子宫内膜碱性磷酸酶的活性；(3)改善微循环；(4)促进子宫运动。

Croton oil induced enhancement of ornithine decarboxylase activities of dorsal epidermis cells in mouse and mouse ear edema model were used to investigate the anti promotion effect of resveratrol.

巴豆油诱发的小鼠耳肿胀和小鼠表皮组织中鸟氨酸脱羧酶的活性来判断其抗促癌作用。

In order to explore the mechanism of the resistance to cucumber damping-off induced by plant growth-promoting rhizobacteria CH1, the physiological and biochemical method was employed to study the changes in activities of Phenylalanine ammonia-lyase, Polyphenol oxidase and contents of ferulic acid, chlorogenic acid, lignin, phenolic compound and flavonoid of phenolic metabolism in cucumber roots pretreated with plant growth-promoting rhizobacteria CH1 and infected by Pythium aphanidermatum.

为研究植物生长促生细菌CH1诱导黄瓜对猝倒病的抗性的作用机制，采用生理生化的方法，测定了黄瓜根部酚类物质代谢中苯丙氨酸解氨酶、多酚氧化酶活性动态及阿魏酸、绿原酸、木质素、总酚、类黄酮含量变化。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Carboxypeptidase inhibitor from potato , a special inhibitor of TAFIa, was used to test effect of heparin and hirudin on activation of TAFI.

对尿激酶诱导的纤溶，水蛭素比肝素有更好的间接促纤溶作用，其原因主要是水蛭素能有效抑制再次血凝的发生，减少了尿激酶和纤溶酶原的短暂性耗竭。

Aqueous extract of cotton has allelopathic effect on Raphanus sativus, Brassica campestris, Lactuca indica, Brassica campestris, Echinochloa crusgall, Setaria viridis and Abutilon theophrasti and its inhibition effect gradually strengthens with increasing concentration. Aqueous extracts from stems, leaves and roots of cotton inhibit or accelerate seed germination and seedling growth, and activity of enzyme of seedling root of cotton. While aqueous extracts from stems and leaves of cotton are 0.02 gmL^(-1), root length, shoot length, lateral root number and dry weight of cotton respectively increase by 10.3%, 3.1%, 22.9% and 2.4% over the control. The above mentioned indexes are lower than the control by 34.4%, 39.2%, 40.6% and 19.0% respectively as the aqueous extracts are 0.1 gmL^(-1). At the same time, root activity and SOD activity decrease by 38.2% and 36.7%, while POD activity and MDA content increase by 52.3% and 36.5%. Growth indexes of plant receptors increase at low concentrations but decrease at high concentrations of aqueous extracts. The allelopathic effect of aqueous extract of stems and leaves is stronger than that of root.

结果表明：棉花植株水浸提液对萝卜、白菜、莴苣、油菜、稗草、狗尾草、苘麻均具有化感效应，随着作用浓度增加，抑制效应逐渐加强；棉花茎叶和根系水浸提液对棉花种子萌发、幼苗生长、幼苗根系活力、根系酶活性有抑制或促进作用，当茎叶浸提液浓度为0.02 gmL^(-1)时，棉花根长、苗高、侧根数、干重分别比对照增加10.3%、3.1%、22.9%和2.4%，当浓度为0.1 gmL^(-1)时，上述指标与对照相比分别降低34.4%、39.2%、40.6%和19.0%，而根系活力、SOD活性比对照降低38.2%、36.7%，POD活性、MDA含量较对照增加52.3%和36.5%，不同浓度棉花植株水浸提液对棉花生长呈现&低促高抑&的规律，且茎叶浸提液作用效应大于根系浸提液。

These results suggested that LMP1-CTAR3 is the important activating domain of inducing cellular proliferation and the domain probably plays the role of mediating to regulate the expression of G-protein, isocitric enzyme, and so on.

以上结果说明，LMP1-CTAR3是其发挥促细胞增殖的重要活性部位，可能通过参与调节G蛋白和异柠檬酸脱氢酶等蛋白质的表达而起作用。

During this years, TCM has a prominent effect on Dysfunctional Dyspepsia,so we study the effect of SiNiSan of soup on the Dysfunctional Dyspepsia sharply from the ability of stomach emptying and the ability of intestines pussing on the normal Kunming rats by the coloring matter,and the chang of the nerve fiber, nerve qualitative, on the stomach organize of normal SD ratsMethods:In the experiment, the thirty Kunming rats, whose avoirdupois are about twenty grammes,and thirty S. D rats, whose avoirdupois are about two hundred grammes,were randomly divided into three groups, 0. 9yS group, SiNiSan of soup group and Mosapride Citrate group.

本课题通过对正常小鼠胃内葡聚糖蓝-2000残留量、小肠内葡聚糖蓝-2000推进率，来研究四逆散对小鼠胃排空、小肠推进的影响；通过采用正常大鼠肠道气囊法，记录正常大鼠用药后肠收缩幅度和频率的变化；采用医学彩色图象全自动分析系统，分析用药后正常大鼠胃窦组织中NOS阳性神经元分布面积和酶活性的变化；采用硝酸酶还原法和比色法，检测用药后正常大鼠胃窦组织中NO和TchE的含量变化，通过以上几个方面，研究四逆散的促胃肠动力作用及其作用机制。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。